WorksheetsApps weeks 6 & 7 review
Total questions: 20
Worksheet time: 11mins
When DNA from two different organisms is combined it is called...
Transgenic DNA
Transformed DNA
Recombinant DNA
Spliced DNA
DNA from one organism can be inserted into another and used to produce a protein because...
DNA is a triplet code
The triplet code is universal
The triplet code is degenerate
The triplet code is non-overlapping
Which enzyme creates a single strand of DNA from mRNA?
Reverse transcriptase
Restriction endonuclease
DNA ligase
DNA polymerase
RNA polymerase
Which enzyme cuts double stranded DNA at specific recognition sites?
Reverse transcriptase
Restriction endonuclease
DNA ligase
DNA polymerase
RNA polymerase
Which enzyme joins a target gene of interest into the DNA of a vector?
Reverse transcriptase
Restriction endonuclease
DNA ligase
DNA polymerase
RNA polymerase
Which enzyme produces double stranded DNA from single stranded DNA?
Reverse transcriptase
Restriction endonuclease
DNA ligase
DNA polymerase
RNA polymerase
Which of the following sequences of DNA are palindromic?
GAATTC
GTATAC
GCCGGC
ACGCGT
Which of the following is NOT a way of obtaining a gene of interest?
Using a gene machine
Using reverse transcriptase
Using restriction enzymes
Using electrophoresis
ECOR I is a restriction enzyme that cutes DNA at the following base sequence: GAATTC. How many fragments of DNA would be produced if the restriction enzyme was used on this piece of DNA?
1
2
3
4
Why are restriction enzymes usually found in bacteria?
When a gene of interest is inserted into a plasmid, which of the following would NOT be used as a way of identifying the useful gene?
Autoradiography
Antibiotic resistance genes
A gene probe that produces a fluorescent glow
A primer
If blunt ends are produced as a result of cleaving DNA, what enzyme is used to add chains of nucleotides to the blunt ends?
DNA polymerase
Terminal transferase
DNA ligase
RNA polymerase
The process of putting recombinant plasmids into bacterial cells is called...
Polymerisation
Hybridisation
Ligation
Transformation
Only approximately 1% of bacteria take up recombinant vector during genetic engineering. Why is this the case?
Some plasmids will have re-joined without taking up the useful gene.
Some DNA fragments re-join before they can be joined into a plasmid.
Some bacteria do not take up plasmids despite cooling & heating.
Some bacteria cannot transcribe the proteins into mRNA.
To identify whether bacteria have taken up recombinant plasmids, we can use...
Replica plating
Autoradiography
UV light
Detergent
Which of the following is NOT correct with regards to genetic engineering of insulin?
mRNA from B cells from the islets of Langerhans is used.
Reverse transcriptase is used to make DNA from RNA.
DNA polymerase is used to make double stranded DNA from single stranded DNA.
DNA ligase is used to form hydrogen bonds between complementary base pairs.
Plants can be genetically engineered to be resistant to Basta. Why is this done?
To provide pesticide resistance.
To enable the crop to grow in areas of drought.
To provide herbicide resistance.
To produce resistance to viral disease.
Which of the following statements regarding the genetic engineering of plants to provide Basta resistance is incorrect?
Agrobacterium tumefaciens contain a Ti plasmid.
The T gene on the Ti plasmid results in tumour formation in plants.
Basta prevents ammonia produced during reactions such as photosynthesis from being converted into glutamate.
Phosphinothricine in Basta acts as a competitive inhibitor of glutamine synthetase.
Which of the following regarding the genetic engineering of plants to be resistant to Basta is INCORRECT?
Normally, plants use an enzyme called glutamate synthetase to convert ammonia into glutamine.
The active ingredient in Basta is phosphinothricine
The gene that codes for phosphinothricine acetyl transferase is introduced to bacteria to give plants resistance to Basta
Phosphinothricine acetyl transferase inactivates glutamine synthetase
Which of the following is not an argument for the use of genetic engineering in plants?
It could reduce world hunger by reducing the need to apply pesticides, which lowers costs and protects insect biodiversity.
The nutritional content of food can be increased.
There is the potential to transfer antibiotic resistance to bacteria within the human gut.
Crops could be grown in drier climates, saving water.
