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WorksheetsMSc Sem2 Class 4
Total questions: 12
Worksheet time: 20mins
Select all correct facts about Zinc Finger Nuclease
30 amino acids, Zn+1 ion is present at the base, coordinated with either 4 Cys residues or 2 Met and 2 Arg, often multiple domains occur in a single DNA binding protein
20 amino acids, Zn+2 ion is present at the base, coordinated with either 4 Cys residues or 2 Cys and 2 His, often multiple domains occur in a single DNA binding protein
30 amino acids, Zn+2 ion is present at the base, coordinated with either 4 Cys residues or 2 Cys and 2 His, often multiple domains occur in a single DNA binding protein
30 amino acids, Zn+2 ion is present at the base, coordinated with either 4 Cys residues or 2 Arg and 2 His, often multiple domains occur in a single DNA binding protein
Explain histone acetylation and de-acetylation
HAT - Histone Acetyl Transferase, transfers acetyl group from Acetyl-coA to arginine residues in the core histones like H3 and H4
HDAC - Histone deacetylase, removes acetyl group
HAT - Histone Acetyl Transferase, removes acetyl group from Acetyl-coA to lysine residues in the core histones like H3 and H4
HDAC - Histone deacetylase, removes acetyl group
HAT - Histone Acetyl Transferase, transfers acetyl group from Acetyl-coA to lysine residues in the core histones like H2A and H4
HDAC - Histone deacetylase, removes acetyl group
HAT - Histone Acetyl Transferase, transfers acetyl group from Acetyl-coA to lysine residues in the core histones like H3 and H4
HDAC - Histone deacetylase, removes acetyl group
Explain Histone acetylation and de-acetylation
Acetylation - Makes DNA more accessible, allows transcription to take place
De-acetylation - Makes DNA accessible, stops transcription from taking place
Acetylation - Makes DNA more inaccessible, allows transcription to take place
De-acetylation - Makes DNA inaccessible, stops transcription from taking place
Acetylation - Makes DNA more accessible, allows transcription to take place
De-acetylation - Makes DNA inaccessible, stops transcription from taking place
Acetylation - Makes RNA more accessible, allows transcription to take place
De-acetylation - Makes RNA inaccessible, stops transcription from taking place
Select the correct fact about HATs
There are two types A and B, type A HATs are present in the cytoplasm and have a bromodomain, they acetylate core histones
type B HATs lack bromodomain and acetylate newly synthesized histones in the cytoplasm
There are two types A and B, type A HATs are present in the nucleus and have a bromodomain, they acetylate core histones
type B HATs lack bromodomain and acetylate newly synthesized histones in the cytoplasm
There are two types A and B, type A HATs are present in the nucleus and lack a bromodomain, they acetylate core histones
type B HATs are with bromodomain and acetylate newly synthesized histones in the cytoplasm
There are two types A and B, type A HATs are present in the nucleus and have a bromodomain, they deacetylate core histones
type B HATs lack bromodomain and acetylate newly synthesized histones in the cytoplasm
What do you know about nucleosome sliding?
Is a mechanism of chromatin remodeling, SWI/SNF is a type of protein found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from GTP hydrolyzing.
Is a mechanism of chromatin remodeling, SWI/SNF is a type of protein found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from ATP hydrolyzing.
Is a mechanism of chromatin condensation, SWI/SNF is a type of protein found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from ATP hydrolyzing.
Is a mechanism of chromatin remodeling, proteasomes are found to be involved, loops are created by the DNA translocase activity of the ATPases and thus remodelers are able to move along DNA using the energy from ATP hydrolyzing.
SWI/SNF ________________________
Was first discovered in C.elegans and was found to regulate function of HO gene involved in mating type switching and also in regulation of SUC2 gene that encodes invertase
Was first discovered in yeast and was found to regulate function of SUC2 gene involved in mating type switching and also in regulation of SUC2 gene that encodes invertase
Was first discovered in yeast and was found to regulate function of HO gene involved in mating type switching and also in regulation of SUC2 gene that encodes invertase
Was first discovered in yeast and was found to regulate function of HO gene involved in mating type switching and also in regulation of pik53 gene that encodes invertase
Explain euchromatin and heterochromatin
Euchromatin - Transcriptionally inactive, more condensed.
Heterochromatin - Transcriptionally active region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes
Euchromatin - Transcriptionally active, more condensed.
Heterochromatin - Transcriptionally active region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes
Euchromatin - Transcriptionally inactive, more condensed.
Heterochromatin - Transcriptionally inactive region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes
Euchromatin - Transcriptionally active region, loosely bound by histones, to make it easily accessible to RNA polymerase and other enzymes
Heterochromatin - Transcriptionally inactive, more condensed.
Select all correct correct facts about nucleosome eviction
All members of the SWI/SNF family and only a subset of ISWI remodelers are unable to eject histone dimers.
All members of the SWI/SNF family and all ISWI remodelers are able to eject histone dimers.
All members of the SWI/SNF family and only a subset of ISWI remodelers are able to eject histone dimers.
All members of the SWI/SNF family and only a subset of ISWI remodelers are able to slide on histone dimer
Select all correct facts about histone exchange
The SWR1 complex catalyzes the exchange of nucleosomal H2A for the H2B variant.
Similarly, H3 can be exchanged by H3.3.
The SWR1 complex catalyzes the exchange of nucleosomal H2A for the H2AZ variant.
Similarly, H3 can be exchanged by H3.3.
The ISWI complex catalyzes the exchange of nucleosomal H2A for the H2AZ variant.
Similarly, H3 can be exchanged by H3.3.
The SWR1 complex catalyzes the eviction of nucleosomal H2A for the H2AZ variant.
Similarly, H3 can be exchanged by H3.3.
Pick the correct role of transferrin and ferritin
Ferritin is responsible for metabolizing iron, while transferrin binds to iron and transports it in the blood
Ferritin is responsible for storing iron in a non-toxic form, while transferrin binds to iron and transports it in the blood
Ferritin binds to iron and transports it in the blood while transferrin is responsible for storing iron in a non-toxic form
Ferritin is responsible for storing iron in a non-toxic form, while transferrin binds to iron and transports it outside the cell
Select the correct statement regarding regulation of ferritin expression
When cellular iron levels are low IRPs dissociate from the IREs because iron binds to their allosteric site, allowing translation of ferritin mRNA and leading to increased ferritin synthesis. When cellular iron levels are high,IRPs bind to the IREs in the 5' UTR of ferritin mRNA, inhibiting translation initiation.
When cellular iron levels are low, IRPs bind to the IREs in the 3' UTR of ferritin mRNA, inhibiting translation initiation. When cellular iron levels are high, IRPs dissociate from the IREs because iron binds to their allosteric site, allowing translation of ferritin mRNA and leading to increased ferritin synthesis
When cellular iron levels are low, IRPs bind to the IREs in the 5' UTR of ferritin mRNA, inhibiting translation initiation. When cellular iron levels are high, IRPs dissociate from the IREs because iron binds to their allosteric site, allowing translation of ferritin mRNA and leading to increased ferritin synthesis
When cellular iron levels are low, IRPs bind to the IREs in the 3' UTR of ferritin mRNA, inhibiting translation initiation. When cellular iron levels are high, IRPs dissociate from the IREs because iron binds to their allosteric site, allowing translation of transferritin mRNA and leading to increased ferritin synthesis
Select correct options regarding transferrin expression regulation
When cellular iron levels are low, iron binds to the IRE and disrupts its association with the 5' stem loop thus rendering the mRNA prone to early degradation. When cellular iron levels become high, the 3' UTR of Transferrin mRNA contains another stem loop to which IRE’s bind, protecting transferrin mRNA from early decay ad nuclease action.
When cellular iron levels are low, iron binds to the IRE and disrupts its association with the 3' stem loop thus rendering the mRNA prone to early degradation. When cellular iron levels become high, the 3' UTR of Transferrin mRNA contains another stem loop to which IRE’s bind, protecting transferrin mRNA from early decay ad nuclease action.
When cellular iron levels are low, the 3' UTR of Transferrin mRNA contains another stem loop to which IRE’s bind, protecting transferrin mRNA from early decay ad nuclease action. When cellular iron levels become high, iron binds to the IRE and disrupts its association with the 3' stem loop thus rendering the mRNA prone to early degradation.
loop to which IRE’s bind, protecting ferritinn mRNA from early decay ad nuclease action. When cellular iron levels become high, iron binds to the IRE and disrupts its association with the 3' stem loop thus rendering the mRNA prone to early degradation
