NEW
Font size
WorksheetsBiotechnology Review
Total questions: 56
Worksheet time: 51mins
This is the practice of producing a genetically identical cell or organism
Cloning
Gel Electrophoresis
Genetic Engineering
DNA Fingerprinting
Why is Dolly the sheep considered a clone?
the source of Dolly's DNA is a single cell taken from an adult donor
the DNA molecules in each of Dolly's cells are different
Dolly has a mix of genes from her foster mother and the sheep that donated a nucleus of one of its cells
Dolly is genetically identical to her offspring
Which suspect left the blood stain?
Bob
Sue
John
Lisa
The process that has been used for centruries to create purebred animals such as black labs is called
recombinant DNA
cloning
selective breeding
Problems with cloning are
creating too many embryos that are unneeded
premature aging and a high failure rate
they have a tendency to overpopulate
The insertion of a gene of interest into a bacterial DNA loop is called
recombinant DNA
insertion technique
mutation propagation
Which of the following is an example of a GMO
Bt Corn
Black Lab Puppies
Dolly the sheep
The graphic shown here is called a
PCR
DNA fingerprint
electrophoresis gel
This is the practice of cutting DNA and separating it by the size of the pieces
Cloning
Gel Electrophoresis
Genetic Engineering
DNA Fingerprinting
Which kitten is most likely the offspring of this cat?
1
2
3
4
This is the practice of choosing individuals of a species to mate based on their desirable characteristics
Bacterial recombination
Natural selection
Genetic modification
Selective breeding
Which of these is the correct definition of genetic modification/genetic engineering?
Choosing individuals to mate based on desired characteristics
Observing genetic changes in populations over time based on adaptive traits
Extracting stem cells to differentiate into specialized tissues
Altering the DNA of an organism at the molecular level
During gel electrophoresis, the _______ pieces of DNA move farthest down the gel
lightest
heaviest
ugliest
deadliest
________ are used to ________ DNA pieces by ________
Restriction enzymes, connect, fission
Restriction enzymes, isolate, "snipping"
Plasmids, connect, fission
Plasmids, isolate, "snipping"
Which is a concern of scientists when genetically modifying plants?
Plants will pass on diseases to animals.
There will be a decrease in biodiversity.
Plants will have a longer growing season.
There will be a decrease in revenue for pesticide manufacturers.
Which best describes a controversial issue associated with the use of genetically modified crops?
the use of genetically modified crops to increase potential yield
the short term use of genetically modified crops in famine stricken countries
the development of genetically modified crops which are resistant to herbicides
the long term effects which may arise from the use of genetically modified crops
Bacteria cells can be used to produce insulin for the treatment of diabetes in humans. Which best explains how the bacteria cells are able to produce insulin for humans?
The bacteria have been grown on a petri dish containing insulin.
The bacteria have been genetically engineered to contain the DNA needed to produce insulin.
The bacteria have been exposed to radioactivity to create mutant strains capable of producing insulin.
The bacteria have been grown on a petri dish containing sugar; the bacteria produce insulin in response to the sugar.
Which area of biotechnology would most likely create ethical issues within human society?
insulin production by bacteria
organ cloning for use in transplants
genetic engineering to improve agricultural yields
DNA and forensic testing of crime scene evidence
Which was most likely the earliest way biotechnology was studied by humans?
finding evidence of crimes
making new medicines to cure sicknesses
discovering the cause of hereditary diseases
breeding better varieties of crops and livestock
Which would be reduced as a result of the development of pest-resistant crops?
use of organic fertilizers
use of chemical insecticides
practice of crop rotation techniques
practice of hydroponic farming techniques
Restriction enzymes are analogous to what common school supply?
glue
scissors
paper
highlighter
What is a plasmid?
a section of the human genome
a material that cuts DNA
viral DNA
an accessory piece of DNA found in bacteria
If a linear piece of DNA contains 3 recognition sites for a restriction enzyme. How many pieces will be produced when the restriction enzyme cuts the DNA?
2
3
4
5
6
Sticky ends are produced when the restriction enzyme cuts in a:
staggered manner
straight through the DNA
A plasmid is larger than a bacterial genome
True
False
How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?
1 - through the gene
2 - on either side of the gene
3 - through the gene and on either side of the gene
4 - twice on each side of the gene
What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate backbone?
DNA polymerase
RNA polymerase
ligase
helicase
Clones have the exact same:
Personalities
Physical appearances
Intelligence levels
Genetic material
What was the significance of Dolly the Sheep?
She was the first organism ever cloned
She was the first animals clone made using SCNT.
She was the first animal cloned as an embryo
She was the first animal to be cloned multiple times
How many sheep were required to create Dolly?
3
2
1
0
What did the scientist prove when cloning Dolly?
That anything could be cloned
That adult cell carry workable versions of all genes necessary to produce an entire new organism
You cant really clone anything, its just a lie
You only can clone animals
What is the first cloned mammal?
goat
cat
sheep
cow
There are now companies that will clone your pet cat for a fee.
true
false
It is currently illegal to use SCNT to create a human being in the United States.
true
false
What is the role of the guide RNA?
It helps the cell repair the DNA
ensures that the Cas9 enzyme cuts at the right point in the genome
Recognizes PAM sequences and opens up the DNA
makes a cut across both strands of the DNA
When two organisms that have very similar or the same characteristics are mated resulting in the prevalence of genetic disorders.
Hybridization
Cloning
Inbreeding
Gene Splicing
Adding or deleting segments of genes to correct or get rid of genetic disorders.
Gene Splicing
Genetic Screening
Gene Therapy
Stem Cell Therapy
Engineering food to make crops that can grow in poor soil conditions, resist disease, tolerate drought, repel insects, etc.
Cloning
Genetically Modified Food
Selective Breeding
Transgenic Organisms
True or False-There could be possible benefits to GMOs.
True
False
