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WorksheetsTechniques in Histology
Total questions: 10
Worksheet time: 5mins
What is the purpose of embedding tissue samples in paraffin wax during histological processing?
To fix the tissue
To cut thinner sections
To preserve the tissue structure
To stain the tissue
What is the primary function of fixation in histology?
To stain the tissue
To preserve tissue structure
To embed the tissue in wax
To wash the tissue
What role does a microtome play in the preparation of histological slides?
To stain tissue sections
To fix tissue sections
To cut tissue sections into thin slices
To preserve tissue sections
What is the significance of clearing tissue samples during histological preparation?
To enhance the staining process
To remove excess water from the tissue
To make the tissue transparent for embedding
To fix the tissue structure
Describe the steps involved in Gram staining.
Crystal violet, iodine, decolorizer, and safranin
Safranin, iodine, decolorizer, and crystal violet
Hematoxylin, eosin, decolorizer, and crystal violet
PAS, iodine, decolorizer, and safranin
What is the significance of the iodine step in the Gram staining process?
To act as a counterstain
To enhance the crystal violet stain
To wash away the stain
To fix the safranin stain
Why do Gram-positive bacteria retain the crystal violet stain while Gram-negative bacteria do not?
Gram-positive bacteria have thicker cell walls
Gram-positive bacteria have thinner cell walls
Gram-negative bacteria lack cell walls
Gram-negative bacteria retain the crystal violet
How can you differentiate between Gram-positive and Gram-negative bacteria based on their color after staining?
Gram-positive bacteria appear red; Gram-negative bacteria appear purple
Gram-positive bacteria appear purple; Gram-negative bacteria appear red
Gram-positive bacteria appear blue; Gram-negative bacteria appear green
Gram-positive bacteria appear green; Gram-negative bacteria appear yellow
What is the most common reason a Gram-positive bacterium appears as Gram-negative after staining?
The bacterium lacks a cell wall
Over-decolorization during the Gram staining process
The use of incorrect staining reagents
The bacterium's cell wall has become thinner over time
What is the purpose of using xylene in histological processing?
To fix the tissue
To dehydrate the tissue
To clear the tissue and remove embedding medium
To stain the tissue
