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Microbiology 2117L- Labs 9-11

Total questions: 50

Worksheet time: 38mins

Name
Class
Date
1.
Replicate the following strand of DNA: 
AATCATGGA
a)
AATCATGGA
b)
TTAGTACCT
c)
UUAGUACCU
d)
GGATAUCUA
2.

What are the three components of a nucleotide?

a)

sugar, hydrogen, nitrogen base

b)

sugar, oxygen, nitrogen base

c)

sugar, phosphate, nitrogen base

d)

sugar, phosphate, protein

3.
If a DNA molecule is 18% Guanine which of the following is correct
a)
18% cytosine, 32% adenine, 32% thymine
b)
32% cytosine, 18% adenine, 32% thymine
c)
18% cytosine, 64% adenine, 64% thymine
d)
32% cytosine, 32% adenine, 18% thymine
4.

What are the 4 nitrogen bases in DNA?

a)

adenine, thymine, uracil, and guanine

b)

adenine, thymine cytosine, and guanine

c)

adenine, thymine, cytosine, and uracil

d)

adenine, thymine, cytosine, and glucose

5.

Guanine bonds with ______________.

a)

Adenine

b)

Guanine

c)

Cytosine

d)

Thymine

6.
This picture is illustrating the ____ nature of DNA.
a)
anti-parallel
b)
deoxyribosic
c)
helical
d)
radioactive
7.

Which sequence of DNA bases would pair with this partial strand

CAT TCA CTG

a)

ATG TGA CAG

b)

TAC ACT GTC

c)

GTA AGT GAC

d)

CAT TCA CTG

8.
DNA replication results in two DNA molecules,
a)
each one with two original strands
b)
each one with two new strands
c)
each one with one new strand and one original strand
d)
one with two new strands and the other with two original strands
9.

DNA is built in this direction

a)

5' to 3' (new nucleotides added to the 3' end)

b)

3' to 5' (new nucleotides added to the 5' end)

c)

3' to 5' (new nucleotides added to the 3' end)

d)

5' to 3' (new nucleotides added to the 5' end)

10.

Adenine pairs with

a)

uracil

b)

thymine

c)

cytosine

d)

adenine

11.

The DNA strands are held together

a)

with covalent bonds

b)

with sugar molecules

c)

with hydrogen bonds between bases

12.

Given the template strand, what is the mRNA strand?

5'- GATGAAC -3'

a)

5'- GUUCAUC -3'

b)

5'- CUACUUG -3'

c)

5'- GAACUAC -3'

d)

5'- GTTCATC -3'

13.

Transcription occurs in the _______ of prokaryotes, and in the _________ of eukaryotes.

a)

Cytoplasm ; cytoplasm

b)

Nucleus ; ribosome

c)

Nucleolus ; nucleus

d)

Cytoplasm ; nucleus

e)

Nucleoid ; cytoplasm

14.

What is the central dogma?

a)

DNA --> mRNA --> tRNA --> rRNA

b)

Template strand --> coding strand --> mRNA

c)

Nucleus --> cytoplasm --> ribosome

d)

DNA --> mRNA --> protein

15.

Given the coding strand, what is the template strand?

5'- AGCTTA -3'

a)

5'- ATTCGA -3'

b)

5'- UCGAAU -3'

c)

5'- TAAGCT -3'

d)

5'- TCGAAT -3'

16.

Transcription converts a gene into a double stranded molecule.

a)

True

b)

False

17.

RNA contains which sugar?

a)

deoxyribose

b)

Ribose

c)

glucose

d)

fructose

18.

Which RNA forms part of the ribosomes?

a)

tRNA

b)

mRNA

c)

rRNA

19.

Which RNA carries the message?

a)

mRNA

b)

rRNA

c)

tRNA

20.

Translation occurs in the _______ and requires a(n) _________.

a)

Nucleus; mRNA

b)

Cytoplasm; ribosome

c)

Cytoplasm; RNA polymerase enzyme

d)

Nucleoid; ribosome

21.

The start codon is ______ and it codes for the amino acid _______.

a)

AUG; Methionine

b)

UAG; Cysteine

c)

UGA; ribosomal binding site

d)

AUG; ribosomal binding site

e)

CUG; Leucine

22.

Codons are located on....

a)

DNA

b)

mRNA

c)

tRNA

d)

rRNA

23.

Anti-codons are located on...

a)

DNA

b)

mRNA

c)

tRNA

d)

rRNA

24.

What cellular structure uses RNA as the blueprint to build proteins?

a)

Cytoplasm

b)

Nucleus

c)

Ribosome

d)

Golgi

25.

A codon consists of ____ RNA base(s)

a)

one

b)

three

c)

six

d)

two

26.

What does tRNA do during translation?

a)

it brings a specific segment of DNA to the ribosome for transcription

b)

it brings any amino acid to the ribosome

c)

it brings the ribosomes to the rRNA to initiate translation

d)

it brings a specific amino acid (which depends upon the codon) to the ribosome for translation

27.

How many amino acids are coded for by this sequence of nucleotides in mRNA: AUG GGA ACU CCA

a)

4

b)

2

c)

6

d)

12

28.
What would be the tRNA anticodon for the mRNA codon CAG?
a)
GTC
b)
GUC
c)
CUG
d)
CAG
29.

What signals where translation will end?

a)

introns

b)

termination sequence

c)

stop codon

d)

operons

30.

TRANSCRIBE this DNA sequence: TACGTTACT

a)

AUGCAAUGA

b)

ATGCAATGA

c)

AUGGATUGA

d)

TACGTTACT

31.

TRANSLATE this RNA sequence: AUGCAAUGA

a)

Met-Glu-Stop

b)

Met-His-Stop

c)

Thr-Glu-Stop

d)

Thr-Pro-Stop

32.
The DNA sequence ATCAGCGCTGGC is part of a gene. how many amino acids are coded for by this message?
a)
4
b)
8
c)
12
d)
20
33.
Choose the nucleotide sequence of the RNA strand that would be complementary to the following strand: 
GTAGTCA
a)
UATUAGA
b)
ACGACTG
c)
CAUCAGU
d)
CATCAGT
34.
What amino acid is represented by the codon UUA?
a)
Phenylalanine
b)
Tyrosine
c)
Leucine
d)
Stop codon
35.
What is the correct amino acid sequence for the mRNA code AUGCCAGUAUGA
a)
Met-Pro-Ala-Val
b)
Met-Pro-Val
c)
Tyr-Gly-His
d)
Tyr-Gly-Arg-His
36.
Which is the mRNA molecule that would be transcribed from this DNA template:
TGGCAAGTACGT
a)
ACCGTTCATGCA
b)
UGGCAAGUACGU
c)
UCCGUUCUUGCU
d)
ACCGUUCAUGCA
37.

Select all that apply to these bacterial colonies.

a)

Shape: round

b)

Margin: entire (smooth)

c)

Shape: Irregular

d)

Margin: Undulate

38.

Identify the form (overall shape) of the colony.

a)

round

b)

irregular

c)

filamentous

d)

punctiform

39.

The margins (edges) of this colony are wavy or:

a)

undulate (wavy)

b)

entire

c)

smooth

d)

filamentous

40.

Identify the form (overall shape) of these bacterial colonies.

a)

filamentous

b)

round

c)

irregular

d)

rhizoid

41.

Most of the bacterial colonies we have looked at are dome-shaped in elevation or:

a)

convex

b)

flat

c)

crateriform

d)

umbonate

42.

Bacteria colonies that are greater than 1 mm in diameter are considered ____.

a)

small

b)

large

c)

variable

d)

None of the other options.

43.

Colony morphology involves all of the following traits/characteristics except ...

a)

form

b)

elevation

c)

margins

d)

cell shape

44.

The image shows an example of a(n):

a)

Streak plate

b)

Spread plate

c)

Ordinary inoculation

d)

Stroke inoculation

45.

Round colonies less than 1.5mm in diameter are called:

a)

Circular

b)

Irregular

c)

Rhizoid

d)

Punctiform

46.

Broth characteristic where bacteria can be seen throughout the culture - the culture appears "cloudy".

a)

Sediment

b)

Pellicle

c)

Turbid

d)

Ring

47.

Growth pattern in which bacteria that "love" oxygen float at the top of a broth.

a)

Turbid

b)

Pellicle

c)

Ring

d)

Sediment

48.

What are obligate aerobes?

a)

Organisms in which oxygen is toxic

b)

Organisms that must have oxygen

c)

Organisms that prefer oxygen, but don't necessarily have to have it

d)

Organisms that "breathe" anaerobically (without oxygen), but they are able to survive when oxygen is present

49.

What are aerotolerant anaerobes?

a)

Organisms that must have oxygen

b)

Organisms in which oxygen is toxic

c)

Organisms that prefer oxygen, but don't necessarily have to have it.

d)

Organisms that "breathe" anaerobically (without oxygen), but they are able to survive when oxygen is present

50.

Organisms that prefer oxygen, but don't necessarily have to have it to survive are called:

a)

Obligate aerobes

b)

Obligate anaerobes

c)

Facultative anaerobes

d)

Aerotolerant anaerobes

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