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Molecular Biology

Total questions: 20

Worksheet time: 11mins

Name
Class
Date
1.

Which of the following processes involves the duplication of a cell's DNA, resulting in two identical DNA molecules?

a)

Transcription

b)

Translation

c)

DNA Replication

d)

Cell Division

2.

Which strand is synthesized continuously in the 5' to 3' direction during DNA replication?

a)

Lagging Strand

b)

Okazaki Strand

c)

Template Strand

d)

Leading Strand

3.

Which enzyme is responsible for unwinding the DNA helix during the process of DNA replication?

a)

Ligase

b)

Polymerase

c)

Helicase

d)

Primase

4.

What is the result of UV light absorption by pyrimidine bases in DNA?

a)

Formation of purine dimers

b)

Breakage of the DNA strand

c)

Formation of pyrimidine dimers

d)

Conversion of pyrimidines to purines

5.

Which of the following secondary structures can form within a single strand of RNA due to complementary base pairing?

a)

Double helix

b)

Hairpin-loop structure

c)

Z-DNA

d)

Triple helix

6.

Which type of protein binds to specific DNA sequences and enhances transcription by stimulating the assembly of the basal transcription apparatus?

a)

Transcriptional repressor

b)

Helicase

c)

DNA polymerase

d)

Transcriptional activator

7.

What is the name of the complex that includes RNA polymerase, general transcription factors, and the mediator, and is essential for the initiation of transcription?

a)

Basal transcription apparatus

b)

Ribosome

c)

Replication fork

d)

Enhancer complex

8.

In bacterial cells, what sequence near the 5′ end of mRNA allows ribosomes to attach and initiate translation while transcription is still occurring?

a)

Shine-Dalgarno sequence

b)

Promoter sequence

c)

TATA Box

d)

Kozak sequence

9.

What is the full form of CPSF, a key protein involved in the cleavage and polyadenylation of pre-mRNA?

a)

Cytoplasmic Polyadenylation Stimulating Factor

b)

Cleavage and Polyadenylation Specificity Factor

c)

Chromatin Packaging and Signal Factor

d)

Cytosine Phosphorylation Synthase Factor

10.

A molecule that transports amino acids to the ribosome and interacts with codons in mRNA, placing amino acids in their proper order in protein synthesis is:

a)

mRNA

b)

miRNA

c)

SiRNA

d)

tRNA

11.

Which of the following best describes a codon?

a)

A sequence of amino acids that determines a protein's structure.

b)

A group of nucleotides in DNA that specifies a single amino acid in a protein.

c)

A segment of RNA that carries the genetic code from the nucleus to the ribosome.

d)

A sequence of proteins that acts as a signal for cellular transport.

12.

What term describes the phenomenon where more than one codon can specify the same amino acid?

a)

Genetic variation

b)

Degeneracy of the code

c)

Amino acid diversity

13.

What is the process called in which tRNAs bind to their respective amino acids?

a)

tRNA synthesis

b)

Codon recognition

c)

tRNA charging

d)

Amino acid activation

14.

What enzymes are responsible for ensuring the specificity between an amino acid and its tRNA?

a)

Ribozymes

b)

DNA polymerases

c)

Aminoacyl-tRNA synthetases

d)

RNA helicases

15.

What term describes a group of bacterial structural genes that are transcribed together, along with their promoter and additional sequences that control transcription?

a)

Operon

b)

Gene cluster

c)

Transcription unit

d)

Expression system

16.

What term describes operons in which transcription is normally active and must be turned off by a repressor mechanism?

a)

Inducible operons

b)

Repressible operons

c)

Constitutive operons

d)

Transcriptional operons

17.

Which protein is responsible for the active transport of lactose into E. coli cells?

a)

Lactase

b)

Repressor

c)

Permease

d)

β-galactosidase

18.

Which operon in E. coli, responsible for the biosynthesis of the amino acid tryptophan, is an example of a negative repressible operon?

a)

Lac operon

b)

Ara operon

c)

Tryptophan (trp) operon

d)

His operon

19.

What term describes DNA sequences that block or insulate the effect of enhancers in a position-dependent manner?

a)

Insulator

b)

Silencer

c)

Promoter

d)

Operator

20.

What term describes the epigenetic phenomenon where a heritable change affects gene expression without altering the DNA sequence?

a)

Gene activation

b)

DNA methylation

c)

Histone modification

d)

Gene silencing