WorksheetsPCR fundamentals
Total questions: 10
Worksheet time: 5mins
PCR is an acronym for?
Polymerase Chain Reaction
Protein Complex Reaction
Polymerase Chain Reading
In endpoint PCR when is the amplification of DNA analysed?
Before the first cycle
After the first cycle
After the final cycle
What reporter dye is commonly used in real-time PCR to monitor amplification?
Bromophenol Blue
Crystal Violet
SYBR Green
In gene expression analysis, what is the purpose of using a reference gene?
To serve as internal controls
To inhibit gene expression
To amplify target genes
What is the function of a probe in a real-time PCR assay?
To block amplification
To bind to target sequences and generate fluorescence
To degrade target DNA
Which type of PCR technique allows you to quantitate mRNA levels levels in a sample?
End-point PCR
Real-time PCR
Reverse transcription PCR
In real-time PCR what does a low Cq value mean?
High initial quantity of target
Low initial quantity of target
No DNA present
In real-time PCR what is the main purpose of the melt curve analysis?
To determine the size of the PCR product
To check for the specificity of amplification
To increase the amplification rate
What is the role of reverse transcriptase in RT-qPCR?
To amplify RNA
To convert cDNA into RNA
To convert RNA to cDNA
What is the main principle behind SYBR Green detection in real-time PCR?
It binds to single-stranded DNA
It inhibits DNA polymerase activity
It fluoresces when bound to double-stranded DNA
