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Introductory Bacterial Conjugation

Total questions: 5

Worksheet time: 3mins

Name
Class
Date
1.

Which material is shared between two groups in the experiment?

a)

3 sterile inoculating loops

b)

LB plate

c)

E. coli Strain I liquid culture

d)

Permanent marker

2.

In Table 1, what symbol is used to indicate bacterial growth?

a)

(+)

b)

(-)

c)

(x)

d)

(*)

3.

What is the first step in using a new loop and the liquid culture of Strain II?

a)

Place a drop of Strain II 1 inch from the drop of Strain I

b)

Place a drop of Strain II 1/2 inch from the drop of Strain I

c)

Mix Strain I and Strain II directly

d)

Dispose of the loop immediately.

4.

What should be done with the loops after use?

a)

Wash and reuse them

b)

Dispose of them in a disposal container

c)

Leave them on the table

d)

Use them for another experiment.

5.

Fill in the blank: Incubate the plates overnight at ___, or for 24–48 hours at room temperature.

a)

25°C

b)

37°C

c)

50°C

d)

100°C