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Trouble CT

Total questions: 10

Worksheet time: 13mins

Name
Class
Date
1.

Match the following

a)

non-specific silver staining

1.

improper cleaned glassware

b)

MT- grey/unstained muscle fibers

2.

No Bouin's was used

c)

MT-collagen was not seen

3.

rinse the slide for too long

d)

VVG- background is stained blue-grey and the elastic fibers do not contrast with the background.

4.

section was under-differentiated

e)

water droplet seen while looking down microscope

5.

did not dehydrate properly

2.

Place the chemicals in the correct order to prepare the Verhoeff's Hx.

a)

Alc. Hx

b)

10% ferric chloride

c)

iodine

1)
2)
3)
3.

Select the region that indicate the presence of muscle fibers in this Elastic VVG stain.

4.

Identify the tissue element represented by this trichrome stain.

5.

Acceptable or not acceptable?

a)

Acceptable

b)

not acceptable

6.

If you use alum Hx for a Trichrome stain, you would see ​ (a)   nuclear staining.

Choose from the below words
pale
dark
7.

What solution stains muscle, rbcs and cytoplasm red in the trichrome stain?

a)

Bouin's

b)

BS-AF

c)

alc. hx

d)

phosphosmolybdic/phosphotungstic acid

e)

aniline blue

8.

VVG stain- acceptable/not acceptable?

a)

acceptable

b)

not acceptable

9.

Trichrome- acceptable/not acceptable?

a)

acceptable

b)

not acceptable

10.

What could be potential causes for not seeing reticulin fibers in the finished slide?

2 lines