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Antibiogramme

Total questions: 16

Worksheet time: 15mins

Name
Class
Date
1.

What does the disk diffusion method measure?

a)

The pH level of the culture broth.

b)

The temperature of the agar medium.

c)

The zone of inhibition.

d)

The size of bacterial colonies.

2.

What does MIC stand for?

a)

Maximum Inhibitory Concentration

b)

Minimum Infective Concentration

c)

Minimum Inhibitory Concentration

d)

Maximum Infective Concentration

3.

Which of the following is used to determine the exact MIC value on an agar plate?

a)

Disk diffusion

b)

E-Test

c)

Broth dilution

d)

Automated systems

4.

What is the primary role of antibiotic susceptibility testing?

a)

To develop new antibiotics

b)

To determine bacterial sensitivity to antibiotics

c)

To measure antibiotic concentration in plasma

d)

To measure the body's immune response

5.

Which organization provide breakpoints for interpreting AST results?

a)

WHO

b)

CLSI

c)

FDA

d)

EUCAST

6.

What is the main advantage of automated systems like VITEK 2?

a)

Manual data recording

b)

High cost-effectiveness

c)

Reduced need for laboratory equipment

d)

Rapid and standardized results

7.

Which medium is commonly used in the disk diffusion method?

a)

Blood agar

b)

MacConkey agar

c)

Mueller-Hinton agar

d)

Sabouraud agar

8.

The disk diffusion method is also known as the (a)   test.

9.

The lowest antibiotic concentration that inhibits visible bacterial growth is called (a)   .

10.

A (a)   zone of inhibition in a disk diffusion test corresponds to increased bacterial resistance to the antibiotic.

11.

If the MIC of an antibiotic is higher than the established breakpoint, or there is no inhibition zone on the Mulelr-Hinton plate, the bacteria are considered (a)   to that antibiotic.

12.

Explain the importance of antibiotic susceptibility testing in clinical practice and its role in combating antimicrobial resistance.

4 lines
13.

Compare and contrast the disk diffusion method and the broth dilution method for AST.

4 lines
14.

What is the purpose of the E-Test in antibiotic susceptibility testing?

a)

To assess the pH of the medium

b)

To identify bacterial species

c)

To measure the growth rate of bacteria

d)

To determine the minimum inhibitory concentration

15.

Which of the following factors can influence the results of antibiotic susceptibility testing?

a)

All of the above

b)

Inoculum size

c)

Temperature of the agar

d)

Incubation time

16.

What is the significance of the zone of inhibition in the disk diffusion method?

a)

It shows the effectiveness of the antibiotic

b)

It determines the size of bacterial colonies

c)

It indicates the bacterial growth rate

d)

It measures the pH of the agar