WorksheetsMass Spectrometry
Total questions: 25
Worksheet time: 13mins
Mass spectrometry is not a spectroscopy technique because
It absorbs light
It emits light
Does not absorb light
Uses electromagnetic radiation
In Mass spectrometry molecules are bombarded with high energy
Protons
Neutrons
X-ray beams
Electrons
In electron impact ionisation method in MS it produces _____ in the first step
Cation
Anion
Radical
Cation radical
Highest mass to charge peak in Mass spectra is called
Base peak
Molecular ion peak
Isotopic peak
Fragment peak
High abundant fragment peak in Mass spectra is called as
Molecular ion peak
Isotopic peak
Base peak
Fragment peak
Relative abundance of all fragment ions are measured with reference to
Molecular ion peak
Base peak
Isotopic peak
None of the above
Isotopic peak ratio of chlorine containing fragments in Mass spectra will be in
1:1
2:1
3:1
4:1
Isotopic peak ratio of bromine containing fragments in Mass spectra will be in
1:1
2:1
3:1
4:1
Molecular ion peak of ethylamine in Mass spectra will be observed at
Even mass
Odd mass
Fraction mass
Not definite
Identify the base peak in the given Mass spectrum
100
99
82
57
Identify the molecular ion peak and base peak in the Mass spectrum
94
66
77
55
Which statement is true for the following Mass spectrum
Indicates presence of Bromine
Indicates presence of chlorine
Indicates presence of nitrogen
Base peak and molecular ion peak are same
The following spectra indicates presence of
Nitrogen
Chlorine
Bromine
Above elements are absent
Following Mass spectra shows the presence of
Nitrogen
Bromine
Chlorine
None of the above
Mass spectra is mainly useful for finding
Conjugation
Functional groups
Molecular weight
Atomic mass
In mass spectrometer, analyte must be ............................. before analyzed based on mass per charge
decomposed
ionized
fragmented
vaporized
Molecular ions produced by electron impact (EI) will be .......
positively charged with m/z equal to the molecular weight
positively charged with m/z equal to the molecular weight + 1
negatively charged with m/z equal to the molecular weight
negatively charged with m/z equal to the molecular weight - 1
The y-axis (ordinate) in the mass spectrum represents .........
mass per charge of the ions
abundance of the ions
quantity of the analyte
number of fragment ions
The following is the disadvantage of electron impact (EI) ionization.......
No fragmentation occurs
Molecular ion may not be found
Analyte can dissociate into several fragments
Molecular ions are abundant
MS spectrum often indicates ions
TRUE
FALSE
Lighter ions bend easily compared to the heavy ions in MS instrumentation
TRUE
FALSE
What information can you gather from an MS spectrum?
Accurate molecular weight (MW) of sample
Molecular formula (from HRMS)
Structure (from fragmentation pattern)
Isotopic abundance
What is the mechanism of action for MS measurement?
Absorption of radiation
Emission of radiation
Diffraction of radiation
Mass to charge ratio
