WorksheetsBiochemistry: Metabolism Part 2 Glycolysis
Total questions: 171
Worksheet time: 1hrs 23mins
Which phase of glycolysis is referred to as the "investment phase"?
Pyruvate production
Steps 1–3, where ATP is consumed
Steps 4–6, where energy is harvested
Anaerobic respiration
What enzyme phosphorylates glucose in the first step of glycolysis?
PFK-1
Hexokinase
Enolase
Aldolase
What is the role of PFK-1 in glycolysis?
Reversible step in energy harvesting
Rate-limiting enzyme and major regulatory step
Forms NADH
Breaks down pyruvate
What is the net ATP yield from glycolysis per glucose molecule?
4
0
2
3
Which metabolite is produced at the end of glycolysis?
Lactate
Pyruvate
Acetyl-CoA
Glycerol
What process recycles NADH back to NAD+ during anaerobic glycolysis?
Gluconeogenesis
Conversion of pyruvate to lactate
β-oxidation
TCA cycle
Which enzyme converts DHAP to G3P?
Aldolase
GAPDH
Triose phosphate isomerase
Pyruvate kinase
Which step in glycolysis directly generates NADH?
Hexokinase
Enolase
Glyceraldehyde-3-phosphate dehydrogenase
PFK-1
What molecule donates phosphate during substrate-level phosphorylation in glycolysis?
ATP
High-energy intermediates like 1,3-BPG and PEP
GTP
Water
What is the role of enolase in glycolysis?
Phosphorylation of glucose
Dehydration of 2-phosphoglycerate to PEP
ATP formation
Oxidation of pyruvate
Which reaction is catalysed by pyruvate kinase?
3PG → 2PG
PEP → Pyruvate with ATP formation
F6P → F1,6BP
G6P → F6P
What is the purpose of glucose phosphorylation in the first glycolytic step?
Increase glucose concentration
Trap glucose inside the cell
Export glucose
Store NADH
What regulates hexokinase?
Pyruvate
Glucose-6-phosphate (product inhibition)
ADP
Citrate
Why is glycolysis considered irreversible?
Enzymes cannot function in reverse
Three steps release large free energy changes
NADH accumulates
ATP is required
Which enzyme links glycolysis and the TCA cycle?
Enolase
GAPDH
Pyruvate dehydrogenase
Citrate synthase
What cofactor does pyruvate dehydrogenase require?
NADPH
Thiamine pyrophosphate (TPP)
Biotin
FADH₂
Which molecule is a key regulator of glycolysis and gluconeogenesis?
(a)
In gluconeogenesis, which enzyme bypasses pyruvate kinase?
Hexokinase
Pyruvate carboxylase + PEP carboxykinase
Glucokinase
GAPDH
What is the committed step of gluconeogenesis?
G6P → Glucose
Pyruvate → Lactate
OAA → PEP
F6P → F1,6BP
Where does gluconeogenesis primarily occur?
Muscle
Liver
Heart
Brain
What is the function of glycogenin?
Glucose transport
Core protein for glycogen synthesis
Energy sensor
Phosphate donor
How is glycogen phosphorylase regulated?
ATP activation
Activated by AMP, inhibited by ATP and G6P
Inhibited by insulin
Activated by NADPH
What triggers glycogen breakdown in muscle during exercise?
Glucagon
Adrenaline (epinephrine)
Insulin
AMP
Which enzyme removes branches in glycogen?
Glycogenin
Glycogen phosphorylase
Debranching enzyme
Phosphoglucomutase
What molecule is used to elongate glycogen?
Glucose-1-phosphate
G6P
UDP-glucose
Fructose
What enzyme catalyses the conversion of glucose-1-phosphate to glucose-6-phosphate in glycogenolysis?
Glycogen synthase
Phosphoglucomutase
Hexokinase
Glucokinase
Which hormone primarily stimulates glycogenolysis in the liver?
Insulin
Glucagon
Adrenaline
Cortisol
What is the major fate of glucose-6-phosphate in the liver during fasting?
Glycogen synthesis
Gluconeogenesis and blood glucose export
Fatty acid synthesis
Lactate formation
What distinguishes glucokinase from hexokinase?
Inhibited by glucose
Found in muscle
Higher Km and only active at high glucose concentrations
Works during fasting
What is the first committed step of the pentose phosphate pathway?
G6P → F6P
G6P → 6-phosphogluconolactone (via G6PDH)
Pyruvate → lactate
GAP → DHAP
What are the products of the oxidative phase of the pentose phosphate pathway?
ATP and NADH
NADPH and ribulose-5-phosphate
CO2 and acetyl-CoA
G6P and pyruvate
Which tissue heavily relies on the pentose phosphate pathway for NADPH?
Skeletal muscle
Brain
Red blood cells
Kidney
What is the primary function of NADPH in cells?
Glycolysis
Reductive biosynthesis and antioxidant defence
Protein degradation
DNA transcription
What pathway contributes to nucleotide biosynthesis?
Glycolysis
Pentose phosphate pathway
Citric acid cycle
Fatty acid oxidation
What is the primary input for the pentose phosphate pathway?
Pyruvate
Glucose-6-phosphate
Ribulose
Acetyl-CoA
What is the purpose of the non-oxidative phase of the pentose phosphate pathway?
Produce NADH
Interconvert sugars for glycolysis or nucleotide biosynthesis
Form lactate
Generate ATP
What enzyme links the pentose phosphate pathway to glycolysis?
Aldolase
Enolase
Transketolase
Pyruvate kinase
Which vitamin-derived cofactor is needed for transketolase?
FAD
Thiamine pyrophosphate (TPP)
NADH
Biotin
What happens when G6PDH is deficient in RBCs?
Excess ATP
Increased oxidative stress and haemolysis
Elevated pyruvate
Overactive TCA
What does a high NADPH/NADP+ ratio indicate?
High oxidative stress
Abundant reducing power for biosynthesis
Enzyme inhibition
DNA damage
What are the two main stages of photosynthesis?
Carbon fixation and glycolysis
Light-dependent and light-independent (Calvin cycle)
Fermentation and respiration
NADPH formation and glycolysis
Where does the light-dependent reaction occur in plants?
Cytosol
Thylakoid membrane
Matrix
Outer membrane
What is the primary output of the light-dependent reactions?
Oxygen only
ATP, NADPH, and O₂
Glucose
Lactate
What pigment absorbs light in photosynthesis?
NAD⁺
Chlorophyll
FAD
Pyruvate
What is the role of RuBisCO?
Forms ATP
Photolysis of water
Fixes CO₂ in Calvin cycle
Regenerates NADPH
What is the product of one full turn of the Calvin cycle?
ATP
Fructose
One G3P molecule
Two glucose molecules
Which process generates O₂ in photosynthesis?
CO₂ fixation
Photolysis of water
Glucose oxidation
G3P isomerisation
What powers the Calvin cycle?
Glycolysis
ATP and NADPH from light reactions
Electron transport chain
Glucose hydrolysis
What is the key function of fatty acid synthase?
Breaks down triglycerides
Elongates fatty acid chains from acetyl-CoA
Transports acetyl-CoA
Oxidises glycerol
What is the primary substrate for biosynthesis of palmitate?
Glucose
Acetyl-CoA + NADPH
Pyruvate
Ketone bodies
What enzyme initiates fatty acid synthesis by forming malonyl-CoA?
Fatty acid synthase
Acetyl-CoA carboxylase (ACC)
What is the cofactor required by acetyl-CoA carboxylase?
NAD+
Biotin
FAD
CoA
Where in the cell does fatty acid synthesis occur?
Mitochondrial matrix
Intermembrane space
Cytosol
ER lumen
What inhibits acetyl-CoA carboxylase?
Insulin
Palmitoyl-CoA (product inhibition)
Citrate
NADPH
Which molecule must be transported out of mitochondria to supply acetyl-CoA to the cytoplasm?
Glucose
Pyruvate
Citrate
Lactate
What is the fate of cytosolic citrate in biosynthesis?
Converted to pyruvate
Cleaved to acetyl-CoA and oxaloacetate
Enters glycolysis
Forms NADPH directly
What enzyme regenerates NADPH during fatty acid biosynthesis?
Pyruvate kinase
Malic enzyme (malate → pyruvate + NADPH)
PEPCK
Transaldolase
What enzyme complex builds the fatty acid chain during biosynthesis?
Carnitine acyltransferase
Fatty acid synthase (FAS)
Lipase
Transaminase
How many carbons are added per cycle by fatty acid synthase?
1
2
3
4
What is the final product of mammalian fatty acid synthesis?
Stearate
Oleate
Palmitate (16:0)
Linoleate
What activates acetyl-CoA carboxylase?
Glucagon
Citrate
FADH₂
NAD⁺
What is the function of desaturase enzymes in lipid metabolism?
Oxidise NADH
Introduce double bonds into fatty acid chains
Elongate glucose
Add hydroxyl groups
What are essential fatty acids?
Ones produced during fasting
Must be obtained from the diet
Stored in muscle
Synthesised from glucose
What is the effect of insulin on fatty acid biosynthesis?
Inhibits acetyl-CoA production
Activates acetyl-CoA carboxylase and promotes biosynthesis
Stimulates β-oxidation
Forms ketones
What determines the rate of fatty acid synthesis?
FADH₂ levels
Activity of acetyl-CoA carboxylase
TCA speed
Glycolysis rate
Which amino acid is the precursor to neurotransmitter dopamine?
Tryptophan
Tyrosine
Glycine
Glutamine
What is the precursor for serotonin synthesis?
Phenylalanine
Tryptophan
Tyrosine
Alanine
What enzyme is used in the first step of amino acid biosynthesis?
PFK
Transaminase (aminotransferase)
What cofactor is required by transaminases?
NAD+
Pyridoxal phosphate (PLP, derived from vitamin B6)
FAD
Coenzyme A
What is the main nitrogen donor in transamination?
Glycine
Aspartate
Glutamate
Serine
What is the role of glutamine synthetase?
Makes NADH
Converts glutamate + NH₃ → glutamine for nitrogen transport
Forms succinate
Inhibits TCA
Where is glutaminase most active?
Liver
Kidney and intestinal cells
Muscle
Brain
What is the fate of α-ketoglutarate in amino acid metabolism?
Fat synthesis
Reenters the TCA cycle after transamination
Forms glucose
Blocks ETC
Which pathway disposes of excess nitrogen from amino acids?
Gluconeogenesis
Glycolysis
Urea cycle
Lipogenesis
Which molecule enters the urea cycle carrying nitrogen?
Aspartate
Carbamoyl phosphate
Glucose
GTP
What is the committed step of the urea cycle?
Aspartate → argininosuccinate
Carbamoyl phosphate synthesis by CPS I
Ornithine → citrulline
Urea → ammonia
What is the source of the second nitrogen in urea?
NH₃
Aspartate
Alanine
Fumarate
Which organelle does the urea cycle begin in?
Cytosol
Mitochondria
Nucleus
Golgi
What enzyme converts citrulline to argininosuccinate?
CPS I
Argininosuccinate synthetase
Arginase
Glutaminase
What is the final step of the urea cycle?
Aspartate production
Arginine synthesis
Arginase cleaves arginine → urea + ornithine
Pyruvate formation
Which molecule links nitrogen metabolism to gluconeogenesis?
Citrulline
Alanine
Glycine
Histidine
In the alanine cycle, what organ receives alanine from muscle?
Brain
Kidney
Liver
Heart
In the glucose-alanine cycle, alanine is converted to what in the liver?
Glucose
Pyruvate → glucose via gluconeogenesis
Citrate
Lactate
What is the purpose of the glucose-alanine cycle?
Protein breakdown
Transport nitrogen to liver and regenerate glucose
Make ATP
Detox ketones
What is the purpose of Km in enzyme kinetics?
Denotes active site shape
Substrate concentration at half-maximal velocity
What does a low Km indicate?
Poor affinity
High enzyme-substrate affinity
Slow catalysis
Weak inhibition
What is the Michaelis-Menten equation?
v = [S]/Km
v = Vmax + [S]
v = [Vmax [S]] / (Km + [S])
v = Km/Vmax
What does Vmax depend on?
Substrate type
Km
Enzyme concentration
pKa
What is kcat?
Substrate turnover
Turnover number = number of reactions per enzyme per second
Inhibitor constant
Michaelis constant
What is the formula for catalytic efficiency?
Km × kcat
kcat / Km
Km / kcat
Vmax / Km
What happens to Vmax in competitive inhibition?
Increases
Remains unchanged
Decreases
Doubles
What happens to Km in competitive inhibition?
Decreases
Increases
No change
Vmax falls
What inhibition type binds only to the enzyme-substrate complex?
Competitive
Non-competitive
Uncompetitive
Reversible
Which plot linearises the Michaelis-Menten equation?
Bar chart
Lineweaver-Burk plot (double reciprocal)
Absorbance curve
Sigmoidal
In Lineweaver-Burk, what does the y-intercept represent?
Km
1/Vmax
-1/Km
Vmax
What is the x-intercept on a Lineweaver-Burk plot?
Vmax
-1/Km
Km
-Vmax
What is a limitation of Lineweaver-Burk plots?
Uses linear scale
Exaggerates error at low substrate concentrations
No intercept
No use in inhibition
What does a sigmoidal curve in an enzyme velocity plot suggest?
Michaelis-Menten enzyme
Allosteric regulation and cooperativity
Competitive inhibition
pH change
What is feedback inhibition?
Cofactor inactivation
End product inhibits an earlier enzyme
Denaturation
ATP increase
What is the key assumption in steady-state enzyme kinetics?
Product concentration is constant
ES complex formation and breakdown is constant over time
Substrate increases
Enzyme degrades
Oxidation is the _________ of electrons.
loss
gain
sharing
transfer
Reduction is the ___________ of electrons.
loss
gain
sharing
transfer
Which reaction removes a molecule of phosphate?
Oxidation
Phosphorylation
Dephosphorylation
Reduction
Which reaction adds a molecule of phosphate?
Oxidation
Phosphorylation
Dephosphorylation
Reduction
NADH is in its what form?
Oxidized form
Reduced form
Glycolysis occurs during aerobic or anaerobic conditions.
aerobic
anaerobic
both
neither
How many high-energy electrons are passed to the carrier NAD+ to produce NADH in glycolysis?
2
8
5
4
During glycolysis, glucose is broken down into how many molecules of pyruvate?
2
3
4
1
How many ATP molecules are required to start glycolysis?
Four
Three
Two
One
Which is a product of glycolysis?
2 molecules of pyruvic acid
1 molecule of glucose
NAD+
oxygen
How many ATP molecules are synthesized from 4 ADP molecules?
2
4
6
1
Where does the pyruvic acid produced by glycolysis go next?
the Calvin Cycle
the electron transport chain
the Krebs Cycle
back through the glycolysis process
Glycolysis is the second stage of cellular respiration.
True
False
Glycolysis requires oxygen
True
False
Which of these is broken down into pyruvic acid?
glucose
oxygen
water
After the NAD+ receives electrons, what does it change to?
ATP
NADH
ADP
During glycolysis, glucose is broken down into how many molecules of pyruvic acid?
2
3
4
1
Glycolysis has a net gain of how many ATP molecules?
2
4
6
How many ATP molecules are required to start glycolysis?
Four
Three
Two
One
Finish the phrase: “As the bonds in glucose are _______ and rearranged, energy is __________.”
broken, released
built, trapped
broken, trapped
built, released
Which of these is a reactant of glycolysis?
pyruvic acid
oxygen
glucose
Glycolysis DOES NOT need ATP to occur.
True
False
Glycolysis has the potential to produce lots of energy very quickly.
True
False
The end product of glycolysis are two molecules of pyruvate. How many carbons are in a pyruvate molecule?
(a)
Glycolysis is a metabolic pathways that
I. Produces ATP
II. Produces NAD+
III. Produces pyruvate
IV. Occurs in cytoplasm
I, II, III
I, II, IV
I, III, IV
I, II, III, IV
Phosphorylation on glucose will activate the glucose molecule.
True
False
________ molecules of ATPs are invested and total ________ molecules of ATPs are formed in the end of glycolysis.
One; two
Two; four
Two; two
Four; four
Which reaction adds a molecule of phosphate?
Oxidation
Phosphorylation
Dephosphorylation
Reduction
NADH is in its what form?
Oxidized form
Reduced form
