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LIFE 102 ERS #4

Total questions: 26

Worksheet time: 19mins

Name
Class
Date
1.

Which enzyme is responsible for “uncoiling” the DNA to prevent supercoiling?

a)

Helicase

b)

DNA Polymerase I

c)

Topoisomerase

d)

Ligase

2.

If a mutation was to render DNA ligase nonfunctional, which of the following effects would you expect?

a)

Inability to turn Okazaki fragments into a functional DNA strand

b)

Inability to synthesize new DNA strands

c)

Supercoiling of DNA helix leading to strand damage

d)

Inability to break hydrogen bonds to begin replication

3.

The promoter region and termination sequence are used in translation, NOT transcription.

a)

True

b)

False

4.

Chargaff’s rules state that:

a)

%A = % T

%C = %G

b)

%A = %C

%G = %T

c)

%A = %G

%C = %T

5.

The central dogma describes the process of going from _______ to _________ via ________

a)

mRNA, DNA, translation

b)

DNA, mRNA, transcription

c)

Amino acids, mRNA, translation

d)

Template strand, coding strand, replication

6.

Which of the following most correctly describes translation?

a)

Translation occurs ONLY in the nucleus

b)

Translation is facilitated by ribosomes and takes place in the cytoplasm

c)

Translation results in mRNA

d)

Translation is facilitated by the ER and occurs in the nucleus

7.

T or F: A virus is an infectious cell that can replicate within a host cell

a)

True

b)

False

8.

What is a bacteriophage?

a)

A type of white blood cell that destroys bacteria

b)

A virus that infects and replicates within bacteria

c)

A protein produced by bacteria to defend against viruses

d)

A specialized organelle found in bacterial cells

9.

The _____ step in Transcription reads unwound DNA to build mRNA

a)
Elongation
b)

Termination

c)

Initiation

d)

Prolongation

10.

Which strand is used as a base for making an RNA sequence?

a)

Coding strand

b)

Elongation strand

c)

Template Strand

d)

Termination Strand

11.

tRNA molecules each carry a specific __________ and _________.

a)

Codon, amino acid

b)

Anticodon, amino acid

c)

mRNA, amino acid

d)

mRNA, anticodon

12.

What does it mean for the genetic code to be “redundant”?

a)

More than one anticodon codes for the same amino acid.

b)

More than one codon can code for the same protein.

c)

More than one codon can code for the same amino acid.

d)

More than one amino acid can be coded for by the same codon.

13.

____ mutations have no effect on amino acid produced by a codon.

a)

Nonsense

b)

Missense

c)

Silent

d)

Frameshift

14.

Which of the following produces a frameshift mutation?

a)

An insertion or deletion of 1 nucleotide

b)

Changing an amino acid codon into a stop codon

c)

Replacing one nucleotide with another (eg C -> G)

d)

The full moon

15.

The Lysogenic cycle is when _____ is incorporated into the host cell's chromosomes.

a)

Bacteriophage

b)

Prophage

c)

mRNA

d)

Adenoviruses

16.

The Lysogenic cycle results in cell death, while the Lytic cycle does not

a)

True

b)

False

17.

The lagging strand is synthesized by DNA polymerase 3:

a)

Continuously, 5' - 3'

b)

Discontinuously, 5' - 3'

c)

Continuously, 3' - 5'

d)

Only once ligase has stitched up the leading strand

18.

During post-transcriptional modification, _______ are removed while _____ stay in the mRNA strand.

a)

Polymerases; helicases

b)

Exons; introns

c)

Introns; exons

d)

Cytosines; adenines

19.

The ____________ is added to the 3' end of mRNA to preserve gene content once the strand leaves the nucleus.

a)

3' cap

b)

5' cap

c)

Poly-A tail

d)

EVIL SHADOW SKULL

20.

To make a bunch of copies of a single gene, scientists use:

a)

PCR (polymerase chain reaction)

b)

Plasmids

c)

Gel electrophoresis

d)

DNA sequencing

21.

Any gene from any organism can be transferred into another organism using biotechnology.

a)

True

b)

False

22.

Viruses are specific to particular ____ and _____ (but they can mutate and evolve!)

a)

Species, receptors

b)

Environments, phenotypes

c)

Times, places

d)

Organisms, cells

23.

Retroviruses 'hijack' cell machinery, allowing their genetic information to flow from

a)

DNA -> RNA

b)

RNA -> DNA

c)

protein -> RNA

d)

protein -> DNA

24.

A prion is:

a)

A misfolded protein that gets stuck in the brain

b)

A retrovirus

c)

A misfolded protein capable of causing other proteins to misfold that same way

d)

A virus

25.

Viruses have all of the following EXCEPT:

a)

DNA or RNA

b)

A protein coat or a nuclear envelope

c)

Ribosomes

26.

AAGCTTACG --> ATAGCTTACG

This demonstrates which type of mutation?

a)

Frameshift

b)

deletion

c)

point

d)

missense