All of the following are microanatomical fixatives, EXCEPT:

Best fixative for the nervous system:

Fixative of choice for the preservation of fats:

Helly’s fluid is exactly the same as in Zenker’s fixative, but instead of GLACIAL ACETIC ACID, what component is added:

Fixative recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei:

Excellent microanatomic fixative for pituitary gland, bone marrow and blood containing organs such as spleen and liver:

All mercurial fixing solutions lead to the formation in tissues of diffuse BLACK granules and these mercury deposits must be removed BEFORE STAINING. Removal of mercuric chloride deposit is accomplished by:

Considered to be the MOST RAPID fixative/ recommended for fixing chromosomes, lymph glands and urgent biopsies:

Fixatives used mainly for acid mucopolysaccharides:

Most widely used fixative for electron microscopy:

The process of decalcification is best performed:

Most ideal and most reliable method of determining extent of decalcification:

The fastest chemical solution in decalcifying tissues is:

All of the following remove intracellular and extracellular water from the tissue following fixation, EXCEPT:

A TOXIC dehydrating agent, primarily employed for blood and tissue films and for smear preparation:

Function/s of tetrahydrofuran:

Excessive exposure to this clearing agent may be extremely toxic to man and may become carcinogenic or it may damage the bone marrow resulting to APLASTIC ANEMIA:

Process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill all the tissue cavities:

Simplest, most common and best embedding medium for routine tissue processing:

A semi-synthetic wax used for embedding the eyes:

The DRY celloidin embedding method is employed chiefly for the:

All of the following are substitutes for paraffin wax, EXCEPT:

Melting point of ester wax:

The last container through which tissue pass through in an automatic tissue processor contains:

Microtome knife recommended for frozen sections or for cutting extremely hard and tough specimens embedded in paraffin blocks, using a base-sledge type or sliding microtome:

Removal of gross nicks on the knife edge:

Removal of “burr” or irregularities on the knife edge:

Angle formed between the cutting edge of the microtome knife (27o-32o):

Angle formed between the surface of the block and the cutting edge of the knife (0o-15o):

This type of microtome easily cuts large blocks and serial sections can be obtained with ease because larger knives can be used:

The cryostat is an apparatus used in fresh tissue microtomy. It consists of a microtome, kept inside a cold chamber which has been maintained at a temperature of:

A tissue exposed to short burst of CARBON DIOXIDE for a few minutes will:

Fat cells and enzymes are best demonstrated in:

When trimming tissue block, they must be surrounded by at least __ of wax.

Thickness of paraffin sections for routine histologic procedures:

The following methods are done for drying sections on slide, EXCEPT:

Most probable cause when clearing agent turns milky as soon as the tissue is placed in it:

It is added to Mayer’s egg albumin to prevent the growth of molds:

Adhesive added to the water in the floating-out bath – most convenient alternative to direct coating of slides:

To avoid distortion of the image, the refractive index of the mountant should be near as possible to that of the glass which is:

Deparaffinization of tissue sections is accomplished by passing through:

Coverslips from slides may be removed by immersion in:

Process by which sections are stained with simple aqueous or alcoholic solutions of the dye:

A tissue-mordant-dye complex is needed in:

With this staining technique, the tissue is first overstained, and the excess stain is removed or decolorized from unwanted part of the tissue:

The regressive staining method employs this procedure:

Accelerate or hasten the speed of the staining power and selectivity of the dye:

Substances which aid in attaching a stain or dye to the tissue: