Worksheetshtmle-draft4
Total questions: 143
Worksheet time: 1hrs 12mins
Name
Class
Date
1.
Embryos and Pituitary biopsies
a)
Bouin's solution
b)
Brasil's solution
2.
Endometrial currettings
a)
Bouin's solution
b)
Brasil's solution
3.
Good for Glycogen
a)
Bouin's solution
b)
Brasil's solution
4.
Not for kidney structures, lipid and mucus
a)
Bouin's solution
b)
Brasil's solution
5.
RBC lysis and Ferric Iron is removed from iron pigments
a)
Bouin's solution
b)
Brasil's solution
6.
Reduces or abolishes Fuelgen reaction
a)
Bouin's solution
b)
Brasil's solution
7.
Better and less messy
a)
Bouin's solution
b)
Brasil's solution
8.
Excellent Fixative for Glycogen
a)
Bouin's solution
b)
Brasil's solution
9.
100%
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
10.
Excellent for fixing and dry and wet smears, blood smears and bone marrow tissues; Fixes and dehydrates at the same time
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
11.
For fixing touch preparations
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
12.
For Wright – Giemsa staining
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
13.
Lower concentrations may result to RBC lysis
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
14.
WBCs are inadequately preserved
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
15.
Doesn’t fix Glycogen
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
16.
Preserves Nucleic acids and nucleoproteins (Good for Histochemistry and Enzyme studies)
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
17.
A Strong reducing agent (therefore, No addition of Strong oxidizing agents like Chromic Acid, Potassium Dichromate and Osmium Tetroxide)
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
18.
Recommended for fixing chromosomes, lymph glands and urgent biopsies
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
19.
Rapid in action
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
20.
Brain tissues fixative for diagnosis of Rabies
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
21.
Fixes and dehydrates at the same time
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
22.
Preserves Nissl granules and cytoplasmic granules well
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
23.
Recommended for fixing mucopolysaccharides and nuclear proteins
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
24.
Better reaction in Fuelgen stain than in Carnoy
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
25.
Nuclear and Histochemical fixative
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
26.
used in ice cold temperatures from – 5 Celsius to 4 Celsius
a)
Osnic acid
b)
Osmium tetroxide
c)
Trichloroacetic Acid
d)
Acetone
27.
ADVANTAGES Precipitates proteins, can be used as a weak decalcifying agent
Softens dense fibrous tissues Poor penetrating agent, suitable only for small tissues or bones
a)
Osmic acid
b)
Osmium tetroxide
c)
Trichloroacetic Acid
d)
Acetone
28.
DISADVANTAGES Poor penetrating agent, suitable only for small tissues or bones
a)
Osmium tetroxide
b)
Trichloroacetic Acid
c)
Acetone
29.
ADVANTAGES Fixes conjugated fats and lipids permanently (insoluble)
Preserves cytoplasmic structures like Golgi Bodies and Mitochondria
Fixes Myelin and peripheral nerves Brilliant staining with Safranin
a)
Osmium tetroxide
b)
Trichloroacetic Acid
c)
Acetone
30.
DISADVANTAGES
Prolonged exposure to acid vapor irritates the eyes (Conjunctivitis)
Deposition of black osmic oxide in the cornea (Blindness)
Inhibits Hematoxylin and makes counterstaining difficult
a)
Osmium tetroxide
b)
Trichloroacetic Acid
c)
Acetone
31.
PRECAUTIONS
- Black Osmic Oxide crystals are dissolved in chilly water.
- Tissues must be washed for at least 24 hours in running water to prevent precipitation
a)
Osmium tetroxide
b)
Trichloroacetic Acid
c)
Acetone
32.
ADVANTAGES
- Study of water diffusible enzymes (Phosphatases and Lipases)
- Brain tissues fixatives for diagnosis of rabies
- Solvent for certain metallic salts
a)
Osmium tetroxide
b)
Trichloroacetic Acid
c)
Acetone
33.
DISADVANTAGES
- Inevitable shrinkage and distortion
- Dissolves fat
- Poor Glycogen preservation
- Evaporates rapidly
a)
Osmium tetroxide
b)
Trichloroacetic Acid
c)
Acetone
34.
Most common chrome-osmium acetic acid fixative
a)
Flemming's solution
b)
Flemming's solution without the acetic acid
35.
Nuclear preparation – Chromosomes
a)
Flemming's solution
b)
Flemming's solution without the acetic acid
36.
Permanently fixes fat; Poor penetrating agent
a)
Flemming's solution
b)
Flemming's solution without the acetic acid
37.
Has the tendency to form artefacts (Wash in running water for 24 hours before dehydration) Very expensive
a)
Flemming's solution
b)
Flemming's solution without the acetic acid
38.
Made up of chromic and osmic acid.
a)
Flemming's solution
b)
Flemming's solution without the acetic acid
39.
Recommended for Cytoplasmic structures (Mitochondria)
a)
Flemming's solution
b)
Flemming's solution without the acetic acid
40.
70 to 100%
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
41.
95%
a)
Methyl alcohol
b)
Ethyl alcohol
c)
Isopropyl alcohol
d)
Carnoy's fluid
e)
Newcomer's fluid
42.
For thermal coagulation of tissue proteins Bacteriologic smears
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
43.
Physical agent similar to heat
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
44.
Increase movement of molecules and accelerates fixation rate
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
45.
Decalcification, Electron Microscopy and Immunohistochemistry; Optimum temperature of 45-55 Celsius
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
46.
4% Formaldehyde or Formol Saline Overnight
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
47.
Fresh Frozen Cryostat sections – fixed in Acetone or Formaldehyde and washed in distilled water prior to enzyme staining
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
48.
Osmium tetroxide; Glutaraldehyde; Paraformaldehyde
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
49.
Karnovsky’s Paraformaldehyde-Glutaraldehyde
a)
Heat fixation
b)
Microwave technique
c)
Enzyme histochemistry
d)
Electron microscopy
e)
Electron histochemistry or electrocytochemistry
50.
Size and Thickness
a)
Retarded by
b)
Enhanced by
51.
Presence of Mucus – Wash with NSS
a)
Retarded by
b)
Enhanced by
52.
Presence of Fat – cut in thin sections
a)
Retarded by
b)
Enhanced by
53.
Presence of Blood – organs with blood must be flushed with NSS
a)
Retarded by
b)
Enhanced by
54.
Cold Temperature – inactivates the enzymes
a)
Retarded by
b)
Enhanced by
55.
Size and Thinness
a)
Retarded by
b)
Enhanced by
56.
Agitation
a)
Retarded by
b)
Enhanced by
57.
Cold Temperature
a)
Wash with NSS
b)
cut in thin sections
c)
Flushed with NSS
d)
Inactivates enzymes
58.
Presence of Blood
a)
Wash with NSS
b)
cut in thin sections
c)
Flushed with NSS
d)
Inactivates enzymes
59.
Presence of fat
a)
Wash with NSS
b)
cut in thin sections
c)
Flushed with NSS
d)
Inactivates enzymes
60.
Presence of mucus
a)
Wash with NSS
b)
cut in thin sections
c)
Flushed with NSS
d)
Inactivates enzymes
61.
– placing a previously fixed tissue into another fixative
a)
Secondary fixation
b)
Post-chromatization
c)
Washing out
62.
- (Facilitates and improves demonstration of substances, Special staining and ensure further hardening and preservation)
a)
Secondary fixation
b)
Post-chromatization
c)
Washing out
63.
Secondary fixation with 2.5 to 3% Potassium Dichromate for 1 day to act as a mordant
a)
Secondary fixation
b)
Post-chromatization
c)
Washing out
64.
– removal of excess fixative to improve staining and remove artefacts
a)
Secondary fixation
b)
Post-chromatization
c)
Washing out
65.
removes Excess Chromates (from Kelly’s, Zenker’s and Flemming’s solution); Excess formalin and osmic acid
a)
Tap water
b)
50 to 70% alcohol
c)
Alcoholic iodine
66.
– removes excess Picric acid (from Bouin’s Solution)
a)
Tap water
b)
50 to 70% alcohol
c)
Alcoholic iodine
67.
removes excessive mercuric fixatives
a)
Tap water
b)
50 to 70% alcohol
c)
Alcoholic iodine
68.
Which of the following acid decalcifying agents is the most common and fastest agent? This has 5 to 10% concentration.
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
69.
Which of the following acid decalcifying agents imparts yellow color to a tissue because of the nitrous acid?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
70.
Acid can be removed by 70% alcohol
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
71.
Which acid decalcifying agent is basically comprised of concentrated Nitric acid with 100 mL of distilled water?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
72.
Which acid decalcifying agent involves urgent biopsies, needle and small biopsies, heavy mineralized cortical bone specimens?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
73.
Which acid decalcifying agent is more damaging to tissue antigens for immunohistochemical staining, and enzymes may be totally lost?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
74.
Which acid decalcifying agent is rapid acting, and ideal for urgent biopsies as well?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
75.
Which acid decalcifying agent must be inside a fume hood?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
76.
Which acid decalcifying agent involves addition of Urea crystals and 5% Sodium Thiosulfate to remove the yellow color?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
77.
Which acid decalcifying agent is used on routine biopsies?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
78.
Which acid decalcifying agent decalcifies and softens at the same time?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
79.
Which acid decalcifying agent has good nuclear and Cytoplasmic staining?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
80.
Which acid decalcifying agent include avoiding maceration due to the presence of alcohol and chromic acid?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
81.
Which acid decalcifying agent is slow for dense bones?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
82.
Which acid decalcifying agent involve ammonia for precipitation and drops of Glacial Acetic Acid and addition of Saturated Aqueous Ammonium Oxalate remove precipitation? Reappearance of the precipitate in this case indicate incomplete decalcification.
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
83.
Which acid decalcifying agent is the most rapid decalcifying agent so far? This is recommended for urgent works.
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
84.
Which acid decalcifying agent involve neutralizing the yellow color via 5% Sodium Sulfate, washed with running water for at least 24 hours?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
85.
Which acid decalcifying agent remove acid by 3 changes of 70 to 90% Ethanol?
a)
Nitric acid
b)
Aqueous nitric solution 10%
c)
Formol - nitric acid
d)
Perenyi's fluid
e)
Phloroglucin nitric acid
86.
Which acid decalcifying agent is Inferior to Nitric acid?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
87.
Which acid decalcifying agent is a Good nuclear staining if used in 1% Solution with 70% Alcohol?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
88.
Which acid decalcifying agent is good for cytological staining?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
89.
Which acid decalcifying agent has No washing prior to dehydration
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
90.
Which acid decalcifying agent has decalcification not determined by chemical means?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
91.
Which acid decalcifying agent is a moderate reacting decalcifying agent?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
92.
Which acid decalcifying agent has Better nuclear staining with less tissue distortion, safer to handle than nitric and hydrochloric acid?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
93.
Which acid decalcifying agent has Adding Citrate accelerates the decalcification?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
94.
Which acid decalcifying agent involve Fixatives and Decalcifying agents?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
95.
Which acid decalcifying agent is used For small pieces of bone and teeth and/or Recommended for teeth and small pieces of bone
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
96.
Which acid decalcifying agent is used For immunohistochemical staining?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
97.
Which acid decalcifying agent Requires 5% Sodium Sulfate and washing out to remove acid?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
98.
Which acid decalcifying agent is Recommended for autopsy materials, bone marrow, cartilages and tissues for research purposes?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
99.
Which acid decalcifying agent is Formic acid combined with Aqueous sodium citrate 20%?
a)
Hydrochloric acid
b)
Von Ebner's fluid
c)
Formic acid
d)
Formic acid - Sodium Citrate solution
100.
Which acid decalcifying agent permits good nuclear staining?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
101.
Which acid decalcifying agent doesn’t require washing out?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
102.
Which acid decalcifying agent has excess acid removed by several changes of 90% alcohol?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
103.
Which acid decalcifying agent is not for dense tissues?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
104.
Which acid decalcifying agent is use on minute pieces of bone?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
105.
Which acid decalcifying agent inhibits nuclear staining with hematoxylin?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
106.
Which acid decalcifying agent has degree of decalcification undetermined by chemical methods?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
107.
Which acid decalcifying agent involve formation of insoluble pigments when dehydrated with alcohol? As a result, this require thorough washing.
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
108.
Which acid decalcifying agent is highly corrosive to skin and mucus membranes, as well as carcinogenic?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
109.
Which acid decalcifying agent doesn't include drain disposal as a legitimate option?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
110.
Which acid decalcifying agent doesn’t produce cell or tissue distortion?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
111.
Which acid decalcifying agent has excellent nuclear and cytoplasmic staining?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
112.
Which acid decalcifying agent is too slow to use for routine purposes?
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
113.
Citrate buffer solution with pH 4.5
a)
Trichloroacetic acid
b)
Sulfurous acid
c)
Chromic acid or Flemming's solution
d)
Citric acid
114.
Nitric acid imparts what color to a tissue due to the presence of Nitrous acis?
a)
White
b)
Yellow
c)
Green
d)
Red
e)
Blue
115.
– agents that bind with calcium ions and other salts to form weakly dissociated complexes and facilitate the removal of calcium salts
a)
Chelating agents
b)
Ion Exchange Resin
c)
Electrophoresis
d)
Tissue softeners
116.
Ammonium form of polystrene resin removes calcium from formic acid – containing decalcifying solution.
a)
Chelating agents
b)
Ion Exchange Resin
c)
Electrophoresis
d)
Tissue softeners
117.
Not recommended for fluids containing mineral acids such as nitric acid or hydrochloric acid
a)
Chelating agents
b)
Ion Exchange Resin
c)
Electrophoresis
d)
Tissue softeners
118.
Decalcifying agent is added usually 20-30 times the volume of the tissue. Decalcification is determined by physical means or X-Ray method
a)
Chelating agents
b)
Ion Exchange Resin
c)
Electrophoresis
d)
Tissue softeners
119.
Positively charged calcium ions migrate to the negative electrode. Calcium is therefore effectively removed from the decalcifying agent
a)
Chelating agents
b)
Ion Exchange Resin
c)
Electrophoresis
d)
Tissue softeners
120.
For small bone fragments
a)
Chelating agents
b)
Ion Exchange Resin
c)
Electrophoresis
d)
Tissue softeners
121.
What is the duration of EDTA chelation with small specimens?
a)
1 to 2 weeks
b)
1 to 3 weeks
c)
4 to 6 weeks
d)
6 to 8 weeks
122.
What is the duration of EDTA chelation on dense cortical bones?
a)
1 to 2 weeks
b)
1 to 3 weeks
c)
4 to 6 weeks
d)
6 to 8 weeks
123.
Binds with Calcium and Magnesium
a)
Heparin
b)
EDTA
c)
Sodium Citrate
d)
Sodium Fluoride
124.
Faster action at ph 7 to 7.4; Optimal binding at ph 8 and higher, but may damage alkaline-sensitive protein linkages
a)
Heparin
b)
EDTA
c)
Sodium Citrate
d)
Sodium Fluoride
125.
- Touching or bending the tissues with the fingers to determine the consistency of the tissue
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
126.
- Pricking or probing the tissue with a fine needle or probe
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
127.
- Most expensive yet most ideal
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
128.
- Most sensitive and most reliable
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
129.
- Use of FAXITRON and Kodak X-OMAT X-Ray film
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
130.
- Not for Mercuric chloride fixed tissues – Radio opacity interference.
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
131.
- Simple, reliable and convenient method
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
132.
- Calcium Oxalate precipitate, incomplete decalcification
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
133.
- No Precipitation, complete decalcification
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
134.
- Immersing the bone tissue in either saturated lithium carbonate solution or 5-10% Aqueous solution of Sodium Bicarbonate solution
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
135.
- Rinsing in running tap water
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
136.
- Acid decalcified tissues for frozen sections must be thoroughly washed with water or stored in formol saline (15% Sucrose or Phosphate buffered saline with 15-20% Sucrose at 4 Celsius before freezing.
a)
Physical means to measure the extent of decalcification
b)
Radiologic method to measure the extent of decalcification
c)
Calcium oxalate test
d)
Post decalcification
137.
decalcifying and softener
a)
Perenyi's fluid
b)
Molliflex
c)
2% Hydrochloric acid
d)
1% Hydrochloric acid in 70% Alcohol
138.
Portions are left 12-24 hours in the fluid
a)
Perenyi's fluid
b)
Molliflex
c)
2% Hydrochloric acid
d)
1% Hydrochloric acid in 70% Alcohol
139.
Cut surface of the block may be submerged in the fluid for 1-2 hours before sectioning
a)
Perenyi's fluid
b)
Molliflex
c)
2% Hydrochloric acid
d)
1% Hydrochloric acid in 70% Alcohol
140.
tissue may appear swollen or soapy (but it doesn’t interfere with the processing and staining
a)
Perenyi's fluid
b)
Molliflex
c)
2% Hydrochloric acid
d)
1% Hydrochloric acid in 70% Alcohol
141.
Washing out or Immersion in 4% Aqueous Phenol solution for
a)
12 to 24 hours
b)
1 to 2 hours
c)
1 to 3 days
142.
Portions are left in Perenyi's fluid for
a)
12 to 24 hours
b)
1 to 2 hours
c)
1 to 3 days
143.
- Cut surface of the block may be submerged in the fluid for how long before sectioning?
a)
12 to 24 hours
b)
1 to 2 hours
c)
1 to 3 days
100 %
