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WorksheetsMidterm Phase I Review - MCB 4403
Total questions: 13
Worksheet time: 13mins
You have a mixed bacterial sample containing a species you'd like to isolate eventually. This bacteria grows in low numbers typically due to its slow growth speed, and you are just trying to increase the number of colonies on the plate. What media would be best?
Defined Media
Undefined Media
Selective Media
Enrichment Media
Which of the following features found in mitochondria and chloroplasts provides the strongest evidence for the endosymbiotic theory?
Their ability to perform cellular respiration and photosynthesis
Their reliance on the eukaryotic host for survival
Their double membrane, 70s ribosomes, separate circular genome
Their presence in almost all eukaryotic cells
You using an electron microscope to look at a sample of ancient bacteria to determine if it is Gram-positive or Gram-negative. Looking at the image you see 3 distinct layers between the background and the cytoplasm in the cell. Based on what you know about Gram-positive and Gram-negative cell structure, what is this bacteria?
Gram-positive
Gram-negative
A plasmid is characterized by which of the following properties?
It is typically a linear DNA molecule found in the eukaryotic nucleus
It is extrachromosomal DNA, usually small and circular, replicating independently
It is an RNA molecule responsible for protein synthesis in the cytoplasm
It contains essential genes for bacterial survival and replicates with the main chromosome
In prokaryotic ribosomes, what are the Svedberg units (S) for the small and large subunits, and what are the specific rRNAs contained within the large subunit?
Small: 40S, Large: 60S, containing 18S and 5.8S rRNA
Small: 30S, Large: 50S, containing 16S and 5S rRNA
Small: 30S, Large: 50S, containing 23S and 5S rRNA
Small: 50S, Large: 30S, containing 16S and 23S rRNA
MULTIPLE ANSWERS: In Gram-negative bacteria, the periplasmic space is located ____ and is the site of ____
In the cytoplasm
Between the plasma membrane and outer membrane
DNA replication and protein synthesis
Protection against phagocytosis
metabolic activity and transport proteins
An organism that obtains its energy by oxidizing inorganic compounds and uses CO2 as its carbon source would be classified as a
Photoheterotroph
Chemoorganoheterotroph
Photoautotroph
Chemolithoautotroph
Which of the following is a macronutrient
Manganese
Iron
Phosphorous
Calcium
Which of the following are considered common bacterial shapes
Cocci
Bacilli
Spirilla
Vibrio
Which of the following internal structures are typically found in a prokaryotic cell
Peptidoglycan cell wall
Nucleoid
Mitochondria
Lysosome
Which process utilizes the Krebs cycle to produce ATP?
Aerobic respiration
Fermentation
Anerobic respiration
You have been documenting the growth curve of a yeast you're growing, named W303a. For 24 hours you you take the OD of your sample every 15 minutes and track that in a graph. Around 10 hours in, you see that your OD doesn't change for around 8 test cycles. What stage of the growth curve was your sample in at that point in time?
Exponential (Log) Phase
Stationary phase
Lag phase
Death phase
What type of bacteria would grow in a natural hot spring (85C)
extremophiles
thermophiles
psychrophiles
mesophiles
