WorksheetsMICR W1-W2
Total questions: 89
Worksheet time: 54mins
select the single-cell organisms
bacteria
fungi
protozoa
virus
prions
select ACELLULAR organisms
bacteria
fungi
protozoa
virus
prions
NON PATHOGEN causes NO INFECTION
they can live with you and can't cause disease
True
False
#1 Acute Lower Respiratory Infections cause _ _ million deaths per year
(a)
#2 HIV-AIDS cause _ million deaths per year
(a)
#3 Diarrheal Disease cause _ _ million deaths per year
(a)
#4 Tuberculosis cause _ _ million deaths per year
(a)
#5 Malaria cause _ _ _ million deaths per year
(a)
good things that microbes do:
Vaccines
Insulin
Antibiotics
Gut microbes
Antigen Presentation
Gut Microbiome functions:
improves mental performance & health
defend against infections
promote host immune system
maintains homeostasis
aid in body's recovery during inflammation
for cell membrane structure
for energy storage
for protection cushion
Lipids
Proteins
Carbohydrates
for work or action
structure
cell recognition
energy
Lipids
Proteins
Carbohydrates
for energy
structure
cell recognition
Lipids
Proteins
Carbohydrates
cytoplasm & ribosomes throughout cell
SIMILARITIES OF PROKARYOTES AND EUKARYOTES
DIFFERENCES OF PROKAYOTES AND EUKARYOTES
DNA is genetic material
SIMILARITIES OF PROKARYOTES AND EUKARYOTES
DIFFERENCES OF PROKAYOTES AND EUKARYOTES
Nucleus presence
SIMILARITIES OF PROKARYOTES AND EUKARYOTES
DIFFERENCES OF PROKAYOTES AND EUKARYOTES
cell wall type
SIMILARITIES OF PROKARYOTES AND EUKARYOTES
DIFFERENCES OF PROKAYOTES AND EUKARYOTES
organelles
SIMILARITIES OF PROKARYOTES AND EUKARYOTES
DIFFERENCES OF PROKAYOTES AND EUKARYOTES
bacterium size
1 to 5 micrometer
1 to 5 nanometer
50 to 250 nanometer
50 to 250 micrometer
virus size
1 to 5 micrometer
1 to 5 nanometer
50 to 250 nanometer
50 to 250 micrometer
what is FLUORENCE Microscopy for?
bacteria
virus
protozoa
fungi
rod-shaped
Bacillus / Bacilli
Coccus / Cocci
Vibrio
Spirillum / Spirilla
Spirochete
spherical or round
Bacillus / Bacilli
Coccus / Cocci
Vibrio
Spirillum / Spirilla
Spirochete
curved - rod
Bacillus / Bacilli
Coccus / Cocci
Vibrio
Spirillum / Spirilla
Spirochete
rigid wave
Bacillus / Bacilli
Coccus / Cocci
Vibrio
Spirillum / Spirilla
Spirochete
flexible wave
Bacillus / Bacilli
Coccus / Cocci
Vibrio
Spirillum / Spirilla
Spirochete
pairs
diplo-
strepto-
straphylo-
chain
diplo-
strepto-
straphylo-
grape-like
diplo-
strepto-
straphylo-
purpose of straining technique
to visualize bacteria
to identify bacteria
to differentiate bacteria
to filter bacteria
to tag bacteria colony
stains all bacteria the same color
typically blue
water washes stains
simple stain
gram stain
acid-fast stain
differentiates type of cell wall
simple stain
gram stain
acid-fast stain
targets the identification of mycobacteria
simple stain
gram stain
acid-fast stain
crystal violet
stains all bacteria
no OUTER MEMBRANE
thick cell wall
gram +ve
gram -ve
acid-fact stain
orangey/red
HAS OUTER MEMBRANE (releases toxin)
susceptible to tetracycline
gram +ve
gram -ve
acid-fact stain
red stain is used first
acid/alcohol is used to wash first satin
blue stain is used for all contrast
gram +ve
gram -ve
acid-fact stain
sticky layer of polysaccharide and small proteins
(a)
roles of glycocalyx
protection
adhesion
water balance
penetration
activation
which type of bacteria you can find glycocalyx?
rods
spheres
spirals
waves
Example of Glycocalyx case
Haemophilus Influenzae
Corynebacterium diphtheriae
Neisseria gonorrhoeae
thick
tightly bound
Capsule Gylococalyx
Slime Layer Glycocalyx
thin and flowing
common to opportunistic bacteria
Capsule Gylococalyx
Slime Layer Glycocalyx
colonies of glycocalyx-covered bacteria
makes it difficult to kill
(a)
complex semi-rigid structure
responsible for cell shape
protects against osmotic pressure changes
bacterial cell wall
gram +ve
gram -ve
contains teichoic acid
bacterial cell wall
gram +ve
gram -ve
part of the cell wall in Gram-positive bacteria
target for drugs
made of phosphate and alcohol
high negative charge
(a)
LPS
found in gram -ve
endotoxin which can be dangerous
antigenic
(a)
small circular pieces of BACTERIAL DNA
not essential for life
"emergency gene material"
(a)
Plasmids do not carry antibiotic resistance
True
False
Bacterial (a) are dormant or resistant stage of certain bacterial cells. NOT a form reproduction. The formed when moisture or nutrients supplies are LOW.
Spores are incapable of resistance and living for long periods of time.
True
False
diseases cause by spore formers:
anthrax
tetanus
gas gangrene
c.difficile
botulism
long, thread-like appendages
allow bacteria to move (motility)
Flagella
Pili
Filament
Chemotaxis
found in each flagellum
made of rigid protein subunits
Flagella
Pili
Filament
Chemotaxis
Flagellin
rotates at about 600 rpm (10 rotations per second)
bacteria can move 50 micrometer per second
Flagella
Pili
Filament
Chemotaxis
Flagellin
move bacteria
attracted to favourable conditions
replled by unfavourable
Flagella
Pili
Filament
Chemotaxis
Flagellin
sticky
many, short, hair-like appendages
Flagella
Pili
Filament
Chemotaxis
Pilin
help bacteria
to attach to other bacteria & surface
to transfer genetic material to another bacterial cell
Flagella
Pili
Filament
Chemotaxis
Pilin
protein found in each pilus
Flagella
Pili
Filament
Chemotaxis
Pilin
bacterial movement during FAVOURABLE condition
long tumbles
short runs
short tumbles
long runs
bacterial movement during UNFAVOURABLE condition
long tumbles
short runs
short tumbles
long runs
general term for sticky proteins
(a)
enable bacteria to attach to surfaces
fimbrae
sex pili
horizontal gene transfer
type of bacteria that can transfer DNA from one bacterium to another
fimbrae
sex pili
horizontal gene transfer
process of DNA transfer between bacterium
fimbrae
sex pili
horizontal gene transfer
bacterial growth time
20 mins to 30 hours
30 mins to 20 hours
2 mins to 20 hours
20 mins to 3 hours
temperature requirement for bacteria to grow
35 - 42 celcius
37 - 42 celcius
37 - 40 celcius
35 - 40 celcius
pH requirement for bacteria to grow
7.0 to 7.5
6.0 to 7.5
7.0 to 7.3
6.5 to 7.5
mesophile bacteria grow at a temp of
0-20 celsius
20 - 40 celsius
40 - 90 celsius
psychrophile bacteria grow at a temp of
0-20 celsius
20 - 40 celsius
40 - 90 celsius
thermophile bacteria grow at a temp of
0-20 celsius
20 - 40 celsius
40 - 90 celsius
flagella's size
10-20 µm
0.5-20 µm
0.5-2 µm
10-50 µm
pili's size
10-20 µm
0.5-20 µm
0.5-2 µm
10-50 µm
requires oxygen for LIFE
e.g. TB
obligate aerobes
obligate anaerobes
facultative anaerobes/ aerobes
microaerophiles
can NOT grow in oxygen = toxic for them
obligate aerobes
obligate anaerobes
facultative anaerobes/ aerobes
microaerophiles
can take or leave oxygen
obligate aerobes
obligate anaerobes
facultative anaerobes/ aerobes
microaerophiles
require small amount of oxygen
too much is toxic
obligate aerobes
obligate anaerobes
facultative anaerobes/ aerobes
microaerophiles
bacteria make their own food
Autotrophes
Heterotrophs
Saprobes
Parasites
bacteria that need to eat food
Autotrophes
Heterotrophs
Saprobes
Parasites
eat dead organisms
Autotrophes
Heterotrophs
Saprobes
Parasites
eat LIVE organisms
Autotrophes
Heterotrophs
Saprobes
Parasites
AGAR is ____
SATA
polysaccharide from marine red algae
provides semi-solid surface for bacterial growth
needs added nutrients
digested during usage
includes beef extract, peptone, and sodium chloride
most bacteria grow well
Selective Media
Complex Media
Differential Media
Combined Selective & Differential
high salt agar
7.5 % instead of 0.5%
staphylococci grow well
Selective Media
Complex Media
Differential Media
Combined Selective & Differential
blood agar
most things will grow but differently
e.g. some in clusters vs. chains
Selective Media
Complex Media
Differential Media
Combined Selective & Differential
has mannitol & salt agar
Selective Media
Complex Media
Differential Media
Combined Selective & Differential
cell count based on light scatter
con: can't tell if dead or alive
(a)
used to separate many bacteria
Direct Microscope Count
Standard Plate Method
Streak Plate Method
Pour Plate method
used to count # of variable CFUs (Colony Forming Units)
Direct Microscope Count
Standard Plate Method
Streak Plate Method
Pour Plate method
