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WorksheetsQuiz1_IBD
Total questions: 40
Worksheet time: 37mins
The building blocks, or subunits of proteins are called
polymers.
amino acids.
monomers.
peptide bonds
Which portion of an amino acid provides its unique characteristics to the amino acid?
alpha carbon
R group
carboxyl group
amino group
Identify the type of bond that links amino acids in a protein.
hydrogen
peptide
glycosidic
ester
Choose the diagram that represents a quaternary protein structure?
Haemoglobin is a quartenary protein because;
It has more than one peptide bonds
Its shape is globular
It consists of more than one polypeptide chains
It has iron in each heme group
Which series is arranged in order from smallest to largest?
Nucleus, chromosome, cell, nucleotide, DNA
nucleotide, DNA, chromosome, nucleus, cell
Chromosomes, nucleus, cell, DNA, nucleotide
Cell, nucleus, chromosome, DNA, nucleotide
Two people each have a gene that codes for a form of hemoglobin. Person #1 has a gene that encodes for normal hemoglobin, whereas Person #2 has a gene that encodes a mutated form of hemoglobin Sickle Cell Anemia. The difference between these two genes is due to
the change of sugar in the molecule
the addition of a nitrogen base
the sequence of nitrogen bases
the broken covalent bonds between the phosphate and sugar
Which of the following statements are true about DNA structure?
The DNA backbone ("sides of the ladder") is made up of sugars and phosphates.
The sugars and phosphates make up the unique code of each gene.
The DNA backbone is the same for every person.
Bases and sugars pair together to form the "rungs" of the DNA ladder.
The sequence of bases is unique for each person.
Which of the following base pairs is matched correctly?
Guanine and Thymine
Cytosine and Adenine
Adenine and Thymine
Thymine and Cytosine
Which of the following statements are true about DNA structure?
The DNA backbone ("sides of the ladder") is made up of sugars and phosphates.
The sugars and phosphates make up the unique code of each gene.
The DNA backbone is the same for every person.
Bases and sugars pair together to form the "rungs" of the DNA ladder.
The sequence of bases is unique for each person.
What is the purpose of Polymerase Chain Reaction (PCR) in the process of sequencing DNA?
To separate DNA fragments by size
To synthesize copies of template DNA
To change the structure of template DNA
To terminate the elongation of a DNA strand
During PCR, what happens during the Denaturation step?
DNA strands are separated according to size using gel electrophoresis.
Primers form base pairs with the single-stranded template DNA.
DNA polymerase adds new nucleotides to the primer to elongate the new strand.
Hydrogen bonds between bases are broken using high heat to separate DNA strands.
During which PCR step do primers form complementary base pairs with single-stranded template DNA?
Denaturation
Annealing
Extension
Synthesis
Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?
DNA polymerase
Primers
Deoxyribonucleotides (dNTPs)
Dideoxyribonucleotides (ddNTPs)
DNA template
How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.
Deoxyribonucleotides are used in both PCR and Cycle Sequencing.
Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.
You cannot add new nucleotides to a deoxyribonucleotide.
Deoxyribonucleotides are NOT found in natural living cells.
Dideoxyribonucleotides are labeled with fluorescent tags.
How is DNA synthesis in PCR and Cycle Sequencing the SAME?
Both require DNA polymerase.
Both require primers.
Both require deoxynucleotides (dNTPs).
Both require dideoxynucleotides (dNTPs).
Both generate new DNA products of equal length.
How is DNA synthesis in PCR and Cycle Sequencing DIFFERENT?
Only PCR uses deoxynucleotides.
Cycle Sequencing generates DNA products of different lengths.
Only PCR analyzes DNA products using gel electrophoresis.
Only Cycle Sequencing uses dideoxynucleotides.
DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.
positive, negative
positive, positive
negative, positive
negative, negative
Larger DNA fragments move through a gel _____________ smaller DNA fragments.
faster than
at the same rate as
slower than
What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?
They stop the elongation of that strand.
That double the length of that strand.
They shorten the length of the strand.
They denature the strand.
Place the steps of DNA Sequencing below into their correct order.
A. Detection of fluorescence w/ lasers & sensors
B. PCR w/ fluorescent dideoxynucleotides
C. Separate DNA by size w/ gel electrophoresis
D. Match DNA sequence using BLAST database
A, B, C, D
B, C, A, D
B, A, C, D
D, B, C, A
FASTA Format is a
Sequence format
Structure format
Number format
Text-based format
First fast sequence searching algorithm for .......... a query sequence against a database.
Analysing
Identifying
Detecting
Comparing
GenBank consist of
Structure
Literature data
DNA and derived protein sequences
Raw data
What is the significance of the accession number in GenBank?
It is a unique identifier for each sequence entry.
It indicates the funding source for the research.
It represents the author of the sequence.
It shows the publication date of the sequence.
Which file format is commonly used for storing protein structures in Cn3D?
If a DNA sequence read ATTGCC what would the complimentary DNA strand read?
GAATGG
UAACGG
TAACGG
Which of the following are the primary nucleotide databases?
GENBANK
EMBL
DDBJ
All of the above
EMBL stands for
European Methods in Biological Laboratory
European Molecular Biology Laboraotory
European MicroBiology Laboratory
European data Mining Based Laboratory
Which blood component is responsible for fighting infections?
Red blood cells
White blood cells
Platelets
Plasma
___________ is a protein molecule made in special cells and released into the blood of the circulatory system, which carries the insulin to every cell in the body.
Iron
Niacin
Guanin
Insulin
