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Lesson 6 Part 3 - Central Dogma

Total questions: 40

Worksheet time: 40mins

Name
Class
Date
1.

How many codons equal one amino acid?

a)

1

b)

3

c)

6

d)

9

2.
Nitrogenous bases are joined by which type of bond?
a)
polar bonds
b)
ionic bonds
c)
covalent bonds
d)
hydrogen bonds
3.
Which sequence of DNA bases would pair with this partial strand
ATG TGA CAG
a)
ATG TGA CAG
b)
TAC ACT GTC
c)
GTA AGT GAC
d)
CAT TCA CTG
4.
What is the complimentary mRNA sequence to the DNA sequence A-T-T-G-C-A.
a)
T-A-A-C-G-T
b)
U-A-A-C-G-T
c)
U-A-A-C-G-U
d)
T-A-A-G-C-U
5.
What determines the code, or information, of a DNA molecule?
a)
the shape (structure) of the nitrogen bases
b)
the order (sequence) of the nitrogen bases
c)
the color of the nitrogen bases
d)
the frequency (number) of nitrogen bases
6.
If Chargaff found that a sample of DNA has 20% ADENINE, how much GUANINE would he have determined to be there?
a)
30%
b)
20%
c)
80%
d)
60%
7.
Which enzyme is responsible for making RNA?
a)
DNA polymerase
b)
RNA enzymase
c)
RNA polymerase
d)
RNA proliferase
8.

What is a promoter?

a)

Sequence of DNA at the beginning of the gene that tells RNA polymerase where to start transcription.

b)

Sequence of RNA that tells the ribosome where to start translation.

c)

Sequence of DNA at the beginning of the gene that tells the ribosome where to start transcription

d)

Sequence of RNA that tells RNA polymerase where to start translation.

9.
What amino acid sequence will be produced based on the mRNA codon sequence
5'-AUG-UCU-UCG-UUA-UCC-UUG-3'
a)
met-ser-leu-ser-leu-ser
b)
met-ser-ser-leu-ser-leu
c)
met-leu-phe-arg-glu-glu
d)
met-glu-arg-arg-glu-leu
10.
What is the function of the poly-A tail in mRNA?
a)
to add modified guanin to the 3' end of the mRNA
b)
to indicate the site of translational termination
c)
to code for the binding of RNA polymerase to the DNA
d)
to help protect the mRNA from degradation by hydrolytic enzymes 
11.
How can a person's muscle cells have the same exact DNA sequences as their nerve cells even though the look and perform completely different?
a)
The two different cells become mutated
b)
The proteins expressed in each cell are different
c)
They actually have different DNA in the two types of cells.
d)
The genome of the different cells changes
12.
The anticodon of a particular tRNA molecule is 
a)
complementary to the corresponding mRNA codon.
b)
complementary to the corresponding triplet in rRNA.
c)
the part of tRNA that bonds with a specific amino acid.
d)
catalytic, making the tRNA a ribozyme.
13.
The parts of a gene that do NOT code for amino acids in a protein are called
a)
introns
b)
exons
c)
codons
d)
anticodons
14.

What is the stage of gradual extension of the growing chain of pre-RNA to the final size?

a)

initiation

b)

elongation

c)

termination

d)

processing

15.

Large RNA-protein complex that conducts RNA splicing by removing of intron and joins together the two exons are

a)

RNA polymerase

b)

spliceosome

c)

Primer

d)

Helicase

16.
What would happen if we did not have start codons?
a)
Polypeptide chains would continue to grow
b)
Amino acids would bind to tRNA
c)
Protein synthesis wouldn't occur
d)
mRNA would form a double strand
17.
Which of the following occurs first in translation?
a)
The small ribosomal subunit binds to mRNA
b)
The large ribosomal subunit binds to mRNA
c)
tRNA binds to the mRNA
d)
A peptide bond forms between amino acids
18.

This enzyme is used to remove short RNA bases

a)

Topoisomerase

b)

DNA Helicase

c)

Exonuclease

d)

Telomerase

19.

If the mRNA codon is AUG, what would the matching tRNA anticodon be?

a)

UAC

b)

AUG

c)

TAC

d)

GUA

20.

The stop codons are: (Choose all that apply)

a)

UAA

b)

UAG

c)

GUC

d)

UGA

21.
The concept that DNA is made of two strands running in opposite directions is called:
a)
Antiparallel
b)
Isomerism
c)
Enantiomerism
d)
S/R Chirality
22.
A particular triple of bases in the coding sequence of DNA is AAA. The anticodon on the tRNA that binds the mRNA codon is
a)
UUU
b)
TTT
c)
AAA
d)
UUA
23.
The first tRNA molecule moves through the __________ sites in the ribosome
a)
A-P-E
b)
P-E
c)
E-P-A
d)
A-P
24.

In eukaryotes, transcription occurs in the nucleus. Where does it occur in prokaryotes?

a)
Endoplasmic reticulum
b)

Nucleoid

c)
Mitochondria
d)
Nucleus
25.

Which DNA strand has the same base sequence as the transcribed RNA (except with T replacing U)?

a)

The template strand

b)

The coding strand

c)

The antisense strand

d)

The non-informational strand

26.

In prokaryotes, which subunit of RNA polymerase is responsible for promoter recognition?

a)
Sigma (σ) factor
b)
Gamma (γ) protein
c)
Beta (β) subunit
d)
Alpha (α) factor
27.

What is the consensus sequence for the prokaryotic promoter element located at the -10 region (the Pribnow box)?

a)

GGGCG

b)

TGTTGACA

c)
TATGAT
d)
TATAAT
28.

In eukaryotes, what is the name of the main protein complex that scans the promoter and recruits RNA polymerase II?

a)

TFIID

b)
Enhancer complex
c)

The spliceosome

d)
Transcription factor
29.

What is the 'transcription bubble'?

a)

the site of DNA replication initiation.

b)

the area where proteins are synthesized.

c)

a structure that stabilizes DNA strands.

d)

the region of unwound DNA where RNA synthesis occurs.

30.

Prokaryotic transcription can terminate in two ways. The 'Rho-independent' mechanism relies on what structure?

a)
A double-stranded DNA structure.
b)
A single-stranded RNA structure.
c)
A protein complex binding site.
d)
A hairpin loop structure in the RNA transcript.
31.

What is '5' capping' in eukaryotic post-transcriptional modification?

a)

The binding of a σ-factor to the 5' end.

b)

The addition of a 7-methyl guanosine to the 5' nucleotide.

c)

The removal of introns from the transcript.

d)

The addition of ~200 Adenine residues to the 3' end.

32.

What is an 'Open Reading Frame' (ORF)?

a)

A sequence of DNA or RNA that can be translated into a protein, starting with a start codon and ending with a stop codon.

b)

A type of RNA that cannot be translated into proteins.

c)

A segment of DNA that only contains non-coding regions.

d)

A sequence of amino acids that does not include start or stop codons.

33.

What is the function of the enzyme 'aminoacyl-tRNA synthetase'?

a)
To synthesize proteins in the ribosome.
b)
To degrade unneeded tRNA molecules.
c)
To transport amino acids across the cell membrane.
d)
To attach the correct amino acid to its corresponding tRNA.
34.

The genetic code is 'degenerate'. What does this mean?

a)
The genetic code is 'degenerate' because it has no specific function.
b)
The genetic code is 'degenerate' as it only uses one codon per amino acid.
c)
The genetic code is 'degenerate' since it is identical across all organisms.
d)
The genetic code is 'degenerate' because multiple codons can specify the same amino acid.
35.

The genetic code is 'unambiguous'. What does this mean?

a)
The genetic code is 'unambiguous' because each codon specifies only one amino acid.
b)
The genetic code is 'unambiguous' since it is the same across all organisms.
c)
The genetic code is 'unambiguous' as it allows for variations in amino acid sequences.
d)
The genetic code is 'unambiguous' because it can code for multiple amino acids.
36.

The catalytic activity of the ribosome (forming the peptide bond) is carried out by...

a)

A protein enzyme in the large subunit.

b)

The rRNA, which acts as a 'ribozyme'.

c)

The mRNA itself.

d)

The EF-Tu elongation factor.

37.

How do prokaryotic ribosomes find the correct place to start translation on an mRNA?

a)

They bind to the 3' polyA tail and move backwards.

b)

They bind to the Shine-Dalgarno sequence, which pairs with the 16S rRNA.

c)

They bind to the Kozak sequence.

d)

They bind to the 5' methylguanosine cap and scan for the first AUG.

38.

During the elongation phase of translation, which factor is responsible for binding the aminoacyl-tRNA to the A site?

a)
Release factor RF-1
b)
Elongation factor EF-Tu (or eEF1A in eukaryotes)
c)
Aminoacyl-tRNA synthetase
d)
Peptidyl transferase enzyme
39.

The step in elongation where the ribosome moves one codon down the mRNA is called...

a)
termination
b)
elongation
c)
translocation
d)
initiation
40.

How is translation terminated?

a)

The ribosome reaches the polyA tail.

b)

A special 'stop' tRNA binds to the stop codon.

c)

A Release Factor (RF) protein binds to the stop codon in the A site.

d)

The Shine-Dalgarno sequence is reached.