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Microorganisms & Bacterial Basics

Total questions: 150

Worksheet time: 1hrs 15mins

Name
Class
Date
1.

Which term best describes microorganisms that cause disease in animals?

a)

Pathogens cause disease in animal hosts

b)

Viruses act only as harmless commensals

c)

Normal flora are always nonpathogenic

d)

Vectors are bacteria that aid digestion

2.

What does virulence specifically refer to in microbiology?

a)

Shape classification of bacterial cells

b)

Location of bacteria on animal skin

c)

Presence of a eukaryotic true nucleus

d)

Ability of a microorganism to cause disease

3.

Prokaryotes differ from eukaryotes primarily by which feature?

a)

Undergoing only sexual reproduction cycles

b)

Possessing linear chromosomal structures

c)

Having multiple membrane-bound organelles

d)

Lacking a true nucleus with chromosomes

4.

Which pairing of bacterial cell shape and description is correct?

a)

Vibrio are spring-like squiggly spirals

b)

Spirochetes are comma-like small curves

c)

Rods are spheres with circular profiles

d)

Cocci are spheres with round forms

5.

Which statement best distinguishes commensal from pathogenic bacteria on animals?

a)

Commensals live normally without disease

b)

Pathogens always remain harmless flora

c)

Commensals require eukaryotic chromosomes

d)

Pathogens cannot inhabit skin or intestines

6.

A veterinarian isolates curved comma-shaped bacteria from a canine wound. Which classification fits best?

a)

Bacilli forming elongated cylindrical rods

b)

Vibrio with distinct comma morphology

c)

Spirochete showing long spring-like coils

d)

Cocci forming spherical clustered cells

7.

Which example correctly matches bacterial genus with its typical shape group?

a)

Staphylococcus with cocci spherical cells

b)

Escherichia with spiral helical forms

c)

Salmonella with comma-like vibrio curves

d)

Bordetella with large filamentous threads

8.

Which feature best describes the bacterial cell membrane structure?

a)

A chitin sheet without lipids

b)

A cellulose-rich trilaminar barrier

c)

A single layer of peptidoglycan fibers

d)

A phospholipid bilayer with embedded proteins

9.

What primary role does the bacterial cell membrane serve for the cell?

a)

Selective barrier regulating solute movement

b)

Rigid exoskeleton maintaining shape

c)

Energy storage as glycogen granules

d)

Genetic information storage site

10.

Which solutes most readily cross the bacterial membrane by dissolving in the bilayer?

a)

Large polar peptides and polysaccharides

b)

Very small non‑polar fat‑soluble molecules

c)

Hydrophilic amino acids and organic acids

d)

Charged inorganic salts and ions

11.

A drug disrupts the phospholipid bilayer of a bacterium. What is the most likely outcome?

a)

Temporary growth arrest without damage

b)

Cell death due to barrier failure

c)

Transformation into a cell wall deficient form

d)

Increased peptidoglycan synthesis only

12.

Which statement best compares membrane and cell wall functions in bacteria?

a)

Membrane stores DNA, wall synthesizes proteins

b)

Membrane forms peptidoglycan, wall makes lipids

c)

Membrane provides rigidity, wall controls diffusion

d)

Membrane regulates transport, wall gives shape

13.

The cytoplasm of bacteria is best described as:

a)

Inert gel composed mainly of cellulose fibers

b)

Dry protein scaffold lacking dissolved solutes

c)

Dense lipid matrix with embedded carbohydrates

d)

Aqueous solution of salts, sugars, amino acids, vitamins

14.

Which component is most abundant in Gram-positive bacterial cell walls?

a)

Polysaccharides make up the majority

b)

Outer membrane proteins dominate the layers

c)

Lipopolysaccharide forms the main barrier

d)

Peptidoglycan predominates in the structure

15.

What primary structural feature causes Gram-negative bacteria to have reduced crystal violet retention?

a)

Multiple peptidoglycan layers without LPS

b)

Thin peptidoglycan with an outer LPS layer

c)

Thick peptidoglycan with teichoic acids

d)

Absence of peptidoglycan and membranes

16.

During a standard Gram stain, which dye provides the final visible color in Gram-negative cells?

a)

Methylene blue determines the color

b)

Crystal violet remains as the final dye

c)

Carbol fuchsin gives the final tint

d)

Safranin provides the counterstain color

17.

Which statement best differentiates Gram-positive and Gram-negative cell envelopes?

a)

Gram-positive have thick peptidoglycan; Gram-negative have LPS outer layer

b)

Gram-positive possess LPS outer layer; Gram-negative have teichoic acids

c)

Both have identical peptidoglycan thickness but different cytoplasm

d)

Neither contains peptidoglycan; both rely on proteins

18.

A coccus stains purple after Gram staining. Which inference is most justified about its cell wall?

a)

It contains a dominant LPS outer leaflet

b)

It has thick peptidoglycan with teichoic acids

c)

It has equal layers of LPS and protein

d)

It lacks peptidoglycan and outer membrane

19.

Which step explains why safranin does not alter the appearance of Gram-positive cells after crystal violet?

a)

Crystal violet dissolves the safranin dye

b)

Crystal violet overwhelms the safranin color

c)

Safranin binds only to LPS molecules

d)

Safranin is washed out by alcohol only

20.

Which barrier most limits permeability in Gram-negative bacteria?

a)

Cytoplasmic membrane proteins

b)

Thick peptidoglycan meshwork

c)

Teichoic acid cross-bridges

d)

Lipopolysaccharide-rich outer membrane

21.

Which clinical benefit best explains why Gram staining is used early in infection management?

a)

It narrows likely pathogens for targeted therapy

b)

It definitively identifies the exact bacterial species

c)

It measures antibiotic serum levels in patients

d)

It detects viral particles in mixed infections

22.

Which pair correctly matches Gram reaction with typical bacterial examples?

a)

Gram-negative cocci: Clostridium and Neisseria

b)

Gram-positive cocci: Staphylococcus and Streptococcus

c)

Gram-positive rods: Escherichia and Salmonella

d)

Gram-negative rods: Clostridium and Vibrio

23.

Some antibiotics, such as cefazolin, are primarily effective against which group?

a)

Gram-positive bacteria

b)

Gram-negative bacteria

c)

Anaerobic protozoa

d)

Acid-fast bacteria

24.

Which statement best distinguishes endotoxin from exotoxin at the molecular level?

a)

Endotoxin is a secreted enzyme; exotoxin is cell wall lipid

b)

Endotoxin is LPS lipid A; exotoxin is a secreted protein

c)

Endotoxin is DNA fragment; exotoxin is polysaccharide

d)

Endotoxin is peptidoglycan; exotoxin is outer membrane sugar

25.

An animal with fever, malaise, diarrhea, and hemorrhagic shock after gram-negative bacterial lysis is most likely reacting to what component?

a)

Lipopolysaccharide released from outer membrane

b)

Protein exotoxin from intracellular spores

c)

Teichoic acid released from cell wall

d)

Peptidoglycan fragments from gram-positive cocci

26.

Horses exposed to high levels of LPS may develop laminitis. Which reasoning best supports an immediate treatment to limit systemic inflammation?

a)

LPS is neutralized by penicillin binding to transpeptidase

b)

LPS triggers a strong immune response upon bacterial lysis

c)

LPS directly blocks ribosomes to stop host protein synthesis

d)

LPS acts only when bacteria actively secrete it during growth

27.

Which distribution of bacteria commonly contains endotoxin as part of their structure?

a)

Spirochetes such as Leptospira and Treponema

b)

Gram-negative species such as E. coli and Salmonella

c)

Gram-positive species such as Staphylococcus and Bacillus

d)

Acid-fast species such as Mycobacterium and Nocardia

28.

Which role do bacterial ribosomes primarily perform within the cytoplasm?

a)

Synthesize proteins from mRNA templates

b)

Transport nutrients across cell membrane

c)

Duplicate circular chromosomes rapidly

d)

Generate ATP via oxidative phosphorylation

29.

What best describes how prokaryotic flagella enable bacterial motility?

a)

They glide on secreted slime layers only

b)

They whip side to side like cilia beats

c)

They rotate like a propeller to push cells

d)

They extend and retract like actin filaments

30.

Fimbriae in bacteria primarily function to do which of the following?

a)

Adhere to surfaces and host cells

b)

Provide energy through chemiosmosis

c)

Mediate swimming motility in liquids

d)

Store nutrients under starvation

31.

How do pili differ from fimbriae in typical Gram- bacteria that possess them?

a)

Pili are shorter and thinner, more numerous

b)

Pili are only found on Gram+ organisms

c)

Pili are longer and thicker, fewer in number

d)

Pili are identical in size but more flexible

32.

What specific role does a bacterial sex pilus serve during conjugation?

a)

Anchors ribosomes to the inner membrane

b)

Stimulates endospore formation under stress

c)

Transfers genetic material between cells

d)

Imports oxygen for aerobic respiration

33.

Which statement about bacterial endospores is most accurate?

a)

They enable survival through heat, drying, and chemicals

b)

They increase growth rate during nutrient abundance

c)

They serve as reproductive gametes for binary fission

d)

They are fragile structures destroyed by lukewarm water

34.

A bacterium that grows with or without oxygen but prefers using oxygen is classified as which type?

a)

Obligate anaerobe intolerant of oxygen

b)

Facultative aerobe capable of anaerobic growth

c)

Microaerophile requiring minimal oxygen

d)

Obligate aerobe requiring constant oxygen

35.

Which pairing correctly matches a disease with a spore-forming bacterial genus noted for it?

a)

Tetanus — Clostridium species produce spores

b)

Lyme disease — Clostridium species produce spores

c)

Syphilis — Clostridium species produce spores

d)

Tuberculosis — Clostridium species produce spores

36.

Which Gram stain color is characteristic of Gram-positive bacteria?

a)

Colorless after decolorizer

b)

Pink after Gram staining

c)

Green with counterstain

d)

Purple under Gram staining

37.

Compared to Gram-positive bacteria, Gram-negative bacteria have how many plasma membranes?

a)

Three layered membranes

b)

One single membrane

c)

Two concentric membranes

d)

No true membranes

38.

Which feature is present in Gram-negative but absent in Gram-positive bacteria?

a)

Exotoxin dominance

b)

Teichoic acids

c)

Outer LPS membrane

d)

Thick peptidoglycan

39.

Which cell wall description best matches Gram-positive bacteria?

a)

Thick lipid layer, no peptidoglycan

b)

Thin protein layer, no peptidoglycan

c)

Thin peptidoglycan, high lipids

d)

Thick peptidoglycan, low lipids

40.

Binary fission in bacteria results in what immediate outcome for the mother cell?

a)

Two haploid gamete cells

b)

Four diverse daughter cells

c)

One larger mother cell

d)

Two identical daughter cells

41.

If a single E. coli cell starts dividing by binary fission at 8 am and doubles every 20 minutes, approximately how many cells are present by 10 am?

a)

One hundred twenty-eight cells

b)

Thirty-two total cells

c)

Sixty-four total cells

d)

Sixteen total cells

42.

Which statement best compares permeability and penicillin susceptibility between Gram types?

a)

Gram-negative more permeable, more susceptible

b)

Gram-positive more permeable, more susceptible

c)

Both equally permeable, equally susceptible

d)

Gram-positive less permeable, less susceptible

43.

Which structural appendages are shared by both Gram-positive and Gram-negative bacteria, according to the comparison?

a)

Pilus and capsule

b)

Pilus and fimbria

c)

Fimbria and flagella

d)

Capsule and spore

44.

Which best defines a pure bacterial culture in diagnostic microbiology?

a)

Multiple species from a single specimen

b)

Multiple strains of the same species

c)

Single species growing from one isolate

d)

Single species with multiple morphotypes

45.

A fecal sample is plated and shows many colony morphologies. What is the most accurate interpretation?

a)

Sterile sample with no growth issues

b)

Expected mixed culture requiring subculture

c)

Pure culture dominated by pathogens

d)

Contamination that invalidates all results

46.

Why is culture and sensitivity testing performed after isolating a pure culture?

a)

To visualize host epithelial attachment

b)

To confirm sample collection was sterile

c)

To identify which antibiotics kill that isolate

d)

To estimate bacterial load in the specimen

47.

On an agar plate with antibiotic discs, clear zones appear around certain discs. What do these zones indicate?

a)

Regions where pH became alkaline

b)

Areas of nutrient depletion by swarming

c)

Evaporation patterns of the solvent

d)

Susceptibility of the isolate to that drug

48.

A clinician treats a typical canine urinary tract infection using a common antibiotic before lab results return. This practice is best described as:

a)

Broad decontamination of the microbiome

b)

Empirical therapy based on likely agents

c)

Definitive therapy after sensitivity

d)

Prophylaxis to prevent colonization

49.

Which initial step enables bacteria to establish infection in animal tissues?

a)

Immediate toxin release into the blood

b)

Integration of plasmids into host DNA

c)

Rapid spore formation within leukocytes

d)

Colonization by attaching to epithelial cells

50.

Which term best describes bacteria present in the bloodstream that can deposit into tissues and cause infection?

a)

Localized endotoxin shock

b)

Commensal surface colonization

c)

Bacteremia with tissue deposition

d)

Latent intracellular phase

51.

Phagocytized bacteria within neutrophils can still cause disease. What key event releases them to other body sites?

a)

Activation of complement

b)

Completion of opsonization

c)

Neutrophil death and lysis

d)

Closure of epithelial gaps

52.

Antibiotics selectively target bacteria because they focus on structures unique to prokaryotes. Which target provides the clearest selectivity with minimal effect on animal cells?

a)

Peptidoglycan cell wall cross-links

b)

Host DNA polymerase enzymes

c)

General plasma membrane lipids

d)

Mitochondrial-like ribosomes

53.

Penicillins and cephalosporins primarily disrupt which bacterial feature to damage cells while sparing animal cells?

a)

Peptidoglycan cross-linking bonds

b)

Phospholipid bilayer thickness

c)

Outer capsule polysaccharides

d)

Cytoplasmic DNA supercoils

54.

Why are antibiotics that act broadly on plasma membranes often more toxic to animals?

a)

They exclusively block eukaryotic ribosomes

b)

They bypass hepatic metabolism entirely

c)

They always form reactive endotoxins

d)

Plasma membranes are not unique to bacteria

55.

Which pairing correctly matches antibiotic class with primary action on protein synthesis?

a)

Penicillins and cephalosporins—stop tRNA charging

b)

Rifampin and sulfonamides—block 50S binding

c)

Amphotericin B—targets 30S initiation complex

d)

Chloramphenicol and tetracycline—ribosomal inhibition

56.

A dog with severe bacterial infection also has renal compromise. To minimize host toxicity while maintaining efficacy, which antibiotic mechanism is most appropriate?

a)

Inhibit host-like ribosomes broadly

b)

Block RNA transcription in all cells

c)

Target peptidoglycan cross-linking selectively

d)

Increase membrane permeability nonspecifically

57.

Which statement best distinguishes bacteriostatic from bactericidal drugs?

a)

Bacteriostatic disrupt DNA; bactericidal block ribosomes

b)

Bacteriostatic kill spores; bactericidal inhibit spores

c)

Bacteriostatic need dormant cells; bactericidal need growth

d)

Bacteriostatic arrest growth; bactericidal kill bacteria

58.

Bacteriostatic agents require which bacterial state to be effective?

a)

Actively growing cells

b)

Dormant latent cells

c)

Sporulated resting cells

d)

Biofilm-embedded cells

59.

Arrested bacteria by bacteriostatic drugs are primarily cleared by which mechanism?

a)

Host immune system

b)

Bacterial quorum sensing

c)

Drug autolysis action

d)

Environmental oxidation

60.

Which Gram-negative rod is most associated with abscesses and otitis externa?

a)

Klebsiella pneumoniae

b)

Escherichia coli

c)

Proteus mirabilis

d)

Pseudomonas aeruginosa

61.

Borrelia burgdorferi is best described as which shape and Gram category?

a)

Rod, Gram-positive

b)

Spiral, Gram-negative

c)

Vibrio, Gram-negative

d)

Coccus, Gram-positive

62.

Which organism is a Gram-negative vibrio linked to abortion in cattle and sheep?

a)

Brucella canis

b)

Leptospira interrogans

c)

Bordetella bronchiseptica

d)

Campylobacter fetus

63.

Which Gram-negative coccobacillus commonly causes kennel cough?

a)

Salmonella typhimurium

b)

Pasteurella multocida

c)

Neisseria gonorrhea

d)

Bordetella bronchiseptica

64.

Which pairing of bacterium and disease is correct for a Gram-negative rod?

a)

Escherichia coli — anthrax

b)

Proteus mirabilis — tetanus

c)

Salmonella sp. — diarrhea, mastitis

d)

Klebsiella sp. — botulism

65.

Leptospira interrogans serovar canicola is categorized as which shape and Gram status?

a)

Spiral, Gram-negative

b)

Filament, Gram-positive

c)

Rod, Gram-positive

d)

Cocci, Gram-positive

66.

Which Gram-negative coccus is associated with gonorrhea?

a)

Streptococcus equi

b)

Staphylococcus aureus

c)

Neisseria gonorrhea

d)

Nocardia asteroides

67.

Which Gram-positive coccus is linked with mastitis and MRSA?

a)

Streptococcus equi

b)

Staphylococcus intermedius

c)

Staphylococcus aureus

d)

Streptococcus agalactiae

68.

Staphylococcus pseudintermedius (S. intermedius group) is most associated with which condition?

a)

Kennel cough in dogs

b)

Listeriosis in poultry

c)

Tetanus in horses

d)

Pyoderma and MRSA/MRSI

69.

Which Gram-positive coccus is the usual cause of gingivitis in dogs?

a)

Streptococcus canis

b)

Streptococcus agalactiae

c)

Streptococcus equi

d)

Staphylococcus aureus

70.

Which bacterium is a Gram-positive coccus causing equine strangles?

a)

Staphylococcus aureus

b)

Streptococcus agalactiae

c)

Neisseria gonorrhea

d)

Streptococcus equi

71.

Bacillus anthracis is best identified as which shape and disease association?

a)

Coccus, mastitis disease

b)

Rod, anthrax disease

c)

Filament, nocardiosis disease

d)

Rod, botulism disease

72.

Which Gram-positive rod produces a neurotoxin causing botulism?

a)

Clostridium tetani

b)

Clostridium botulinum

c)

Mycobacterium tuberculosis

d)

Listeria monocytogenes

73.

Clostridium perfringens is most associated with which clinical syndrome?

a)

Gingivitis in canines

b)

Equine strangles

c)

Pulmonary tuberculosis

d)

Clostridial diarrhea

74.

Which pairing correctly matches organism, morphology, and disease?

a)

Nocardia asteroides — rod — botulism

b)

Mycobacterium tuberculosis — filament — mastitis

c)

Clostridium tetani — rod — tetanus

d)

Listeria monocytogenes — coccus — listeriosis

75.

Nocardia asteroides is best described by which morphology and disease pattern?

a)

Coccus, strangles and gingivitis

b)

Spiral, leptospirosis and diarrhea

c)

Rod, anthrax and MRSA

d)

Filament, nocardiosis and mastitis

76.

Which Gram-positive rod is primarily associated with listeriosis?

a)

Listeria monocytogenes

b)

Bacillus anthracis

c)

Mycobacterium tuberculosis

d)

Clostridium perfringens

77.

Which statement best explains why viruses are often considered nonliving?

a)

They synthesize ATP independently of cells

b)

They metabolize lipids using viral enzymes

c)

They cannot reproduce without a host cell

d)

They respire without oxygen in all hosts

78.

What is the correct relationship among capsid and capsomeres?

a)

Capsomeres are nucleic acids within capsids

b)

Capsid is many capsomeres forming the protein coat

c)

Capsomeres are lipid units forming viral envelopes

d)

Capsid is an enzyme coating the viral genome

79.

Which feature distinguishes enveloped viruses from naked viruses?

a)

Presence of double-stranded nucleic acid only

b)

Presence of host-independent replication enzymes

c)

Presence of an outer membrane around the capsid

d)

Presence of capsomeres arranged as helical coils

80.

A virus sample shows a many-sided protein shell surrounding the genome. Which morphology is most consistent with this description?

a)

Amorphous capsid lacking defined geometry

b)

Complex capsid with tail fibers only

c)

Polyhedral capsid with icosahedral symmetry

d)

Helical capsid with loosely wound coil

81.

You examine electron micrographs showing rod-like particles with a tightly wound coil of nucleic acid. Which virus architecture does this indicate?

a)

Helical capsid enclosing a coiled genome

b)

Polyhedral capsid forming many-sided shells

c)

Naked capsid lacking protein subunits

d)

Enveloped capsid with matrix proteins

82.

A researcher proposes that viral enzymes always enable viruses to replicate without entering host cells. Which evaluation is most accurate?

a)

Inaccurate because naked viruses contain no nucleic acid

b)

Accurate because viral enzymes replace host organelles

c)

Accurate because envelopes provide energy for synthesis

d)

Inaccurate because replication still requires host machinery

83.

Which statement best describes complex viral morphology typically seen in bacteriophages?

a)

Amorphous shape lacking defined structural parts

b)

Only helical symmetry with no tail fibers

c)

A head and tail combining helical and polyhedral

d)

Only polyhedral symmetry with no sheath

84.

Enveloped viruses are characterized by which feature regardless of internal symmetry?

a)

An RNA core lacking any capsomeres

b)

A rigid protein sheath around the tail

c)

A double capsid without surface proteins

d)

An outer lipid envelope surrounding the capsid

85.

Which virus from the list is an example of a helical enveloped virus?

a)

Rabies virus with a lipid envelope

b)

Herpesvirus with an icosahedral envelope

c)

Coronavirus with a polyhedral envelope

d)

Equine encephalitis virus nonenveloped

86.

Which size range correctly matches a very small animal virus mentioned in the material?

a)

Bacteriophages consistently five nanometers

b)

Parvoviruses around one hundred nanometers

c)

Porcine circovirus around seventeen nanometers

d)

Influenza viruses always under ten nanometers

87.

A newly imaged virus shows an icosahedral capsid enclosed by a membrane studded with spikes. Which classification choice is most justified for this morphology?

a)

Complex bacteriophage morphology

b)

Enveloped polyhedral virus morphology

c)

Naked helical virus morphology

d)

Amorphous nonviral particle morphology

88.

Which statement best distinguishes DNA and RNA viral genomes relevant to replication enzymes?

a)

RNA viruses never encode their own polymerases

b)

DNA viruses often use DNA-dependent polymerases

c)

DNA viruses universally carry reverse transcriptase

d)

RNA viruses replicate using host RNA-dependent DNA polymerase

89.

A virus with a double-stranded RNA genome must perform which immediate step after entry?

a)

Convert RNA to DNA using reverse transcriptase

b)

Ligate plus and minus strands to circular DNA

c)

Directly translate genomic RNA with host ribosomes

d)

Transcribe mRNA using RNA-dependent RNA polymerase

90.

For a positive-sense single-stranded RNA virus, what is the first productive event in the cytoplasm?

a)

Synthesis of complementary DNA intermediate

b)

Packaging of RNA into preformed capsids

c)

Translation of genomic RNA as mRNA

d)

Nuclear import and genome circularization

91.

Negative-sense single-stranded RNA viruses require what to be packaged within the virion?

a)

Reverse transcriptase for cDNA synthesis

b)

DNA-dependent RNA polymerase II

c)

Host ribosomes for immediate translation

d)

RNA-dependent RNA polymerase enzyme

92.

Which pathway correctly matches genome type to mRNA relationship?

a)

ssDNA genomes are directly translated as mRNA

b)

dsRNA genomes are spliced by host RNAP II

c)

−ssRNA genomes serve directly as cellular mRNA

d)

dsDNA produces mRNA via host or viral RNAP

93.

Single-stranded DNA viruses typically must first generate which intermediate?

a)

Double-stranded DNA form for transcription

b)

Double-stranded RNA replicative form

c)

Circular minus-sense RNA template

d)

Positive-sense RNA ready for translation

94.

Which scenario best explains why many RNA viruses have higher mutation rates than DNA viruses?

a)

RNA-dependent polymerases lack robust proofreading

b)

RNA genomes are shielded by more stable capsids

c)

DNA-dependent polymerases always replicate in cytoplasm

d)

RNA viruses use host DNA repair to fix mismatches

95.

In the diagrammed pathways, which genome type most directly serves as mRNA without prior transcription?

a)

Negative-sense single-stranded RNA

b)

Double-stranded RNA with capped ends

c)

Double-stranded DNA with inverted repeats

d)

Positive-sense single-stranded RNA

96.

Which step immediately follows viral attachment in the typical animal virus replication cycle?

a)

Penetration into the host cell

b)

Assembly of progeny virions

c)

Release through budding or lysis

d)

Synthesis of host enzymes only

97.

During uncoating, what key event occurs inside the infected cell?

a)

Genome integrates into bacterial plasmids

b)

Capsid disassembles releasing nucleic acid

c)

Ribosomes package capsid proteins

d)

Envelope fuses forming exit pores

98.

Which description best matches the biosynthesis phase of viral replication?

a)

Capsid and genome are put together

b)

Viral genome directs synthesis of proteins

c)

Virions bind to specific cell receptors

d)

Mature particles exit the host cell

99.

A drug blocks endocytosis of enveloped viruses. Which replication stage is most directly inhibited?

a)

Assembly of structural proteins

b)

Penetration into the host cytoplasm

c)

Uncoating of the viral genome

d)

Release of assembled virions

100.

Which sequence correctly orders the major stages of a typical animal viral life cycle?

a)

Attachment, penetration, uncoating, biosynthesis, assembly, release

b)

Attachment, assembly, uncoating, biosynthesis, penetration, release

c)

Penetration, attachment, biosynthesis, release, uncoating, assembly

d)

Uncoating, attachment, penetration, release, assembly, biosynthesis

101.

A mutation prevents capsid proteins from interacting with the viral genome. What immediate outcome is most likely?

a)

Failure of assembly into new virions

b)

Failure of attachment to host cell

c)

Failure of genome replication enzymes

d)

Failure of release through cell lysis

102.

In many animal viruses, what is a direct cellular consequence of the release stage?

a)

Host cell damage or death occurs

b)

Host DNA replication accelerates

c)

Host ribosomes become enveloped

d)

Host membrane receptors multiply

103.

Which feature most commonly describes the visible appearance of molds on spoiled food?

a)

Fuzzy and filamentous surface growth

b)

Smooth and glossy surface layer

c)

Wet and slimy uniform coating

d)

Dry and powdery crystal patches

104.

In asexual fission of a fungal cell, what immediate outcome occurs?

a)

A sporangium releases many spores

b)

Two hyphae form a zygospore

c)

A single cell divides into two daughters

d)

A bud detaches from the parent

105.

Which process best matches the description: a daughter cell develops from a small outgrowth of the parent cell?

a)

Spore germination event

b)

Fission producing two cells

c)

Fusion forming gametangia

d)

Budding in yeast reproduction

106.

During sexual fusion in molds, what structure forms at the meeting point of compatible hyphae before zygospore development?

a)

Conidiophore apex vesicle

b)

Ascus with ascospores

c)

Basidium with sterigmata

d)

Gametangia at contact site

107.

Which statement best explains why many fungi are hard to culture in the laboratory?

a)

They tolerate extremes yet adapt slowly in vitro

b)

They require neutral pH exclusively

c)

They only grow in high-salt media

d)

They have diverse nutrient strategies in nature

108.

A veterinarian suspects ringworm in a dog. Which genera are most likely involved?

a)

Rhizopus or Mucor species

b)

Microsporum or Trichophyton species

c)

Candida or Aspergillus species

d)

Cryptococcus or Histoplasma species

109.

Given fungi survive better than many microbes in acidic environments and use varied nutrients, which management choice reduces household mold risk most effectively?

a)

Raise pH of all surfaces routinely

b)

Reduce moisture and improve airflow

c)

Increase humidity and lower ventilation

d)

Add sugar-rich foods to open air

110.

Which systemic mycosis is caused by Blastomyces dermatitidis in animals?

a)

Blastomycosis in multiple organ systems

b)

Histoplasmosis affecting spleen and liver

c)

Dermatophytosis limited to the skin

d)

Cryptococcosis involving nasal passages

111.

Histoplasma capsulatum most commonly produces disease involving which combination of body systems in animals?

a)

Cardiac, vascular, hematologic, lymphatic, endocrine

b)

Renal, endocrine, musculoskeletal, reproductive, integumentary

c)

Dermal, subcutaneous, nail, hair, hoof tissues

d)

Gastrointestinal, liver, spleen, ocular, respiratory, neurologic

112.

Cryptococcosis in animals is best characterized by primary involvement of which site with potential spread to others?

a)

Lymph nodes only without pulmonary involvement

b)

Intestinal mucosa with exclusive splenic colonization

c)

Cutaneous lesions with rapid hepatic dissemination

d)

Respiratory nasal cavity with CNS and ocular spread

113.

A dog presents with respiratory distress, lymphadenopathy, and ocular lesions. Which fungal disease most aligns with this pattern?

a)

Blastomycosis affecting respiratory, lymphatic, ocular

b)

Histoplasmosis restricted to gastrointestinal tract

c)

Dermatophytosis causing hair shaft invasion

d)

Cryptococcosis limited to the skin only

114.

Which statement best differentiates systemic mycoses from superficial fungal infections in animals?

a)

Systemic mycoses always resolve without antifungal therapy

b)

Systemic mycoses are never zoonotic to human handlers

c)

Systemic mycoses remain confined to keratinized tissues

d)

Systemic mycoses disseminate beyond skin to internal organs

115.

A cat with chronic nasal discharge and neurologic signs is suspected of a systemic fungal infection. Which organism is the most likely cause?

a)

Cryptococcus neoformans or Cryptococcus gattii

b)

Microsporum canis causing ringworm

c)

Blastomyces dermatitidis only in the lungs

d)

Histoplasma capsulatum limited to spleen

116.

Which statement best defines a parasitic relationship in animals?

a)

The host benefits while the parasite is harmed

b)

Neither organism is affected by the interaction

c)

The parasite benefits while the host suffers harm

d)

Both organisms benefit equally from interaction

117.

Which group lists common veterinary parasites encountered in practice?

a)

Mites, molds, algae, protozoa

b)

Tapeworm eggs, pollen, dander, dust

c)

Bacteria, viruses, prions, fungi

d)

Intestinal worms, fleas, ticks, heartworms

118.

What is the correct definition of toxicology in veterinary medicine?

a)

The study of immune responses to vaccines

b)

The study of parasite lifecycles and transmission

c)

The study of toxins and treatment of intoxications

d)

The study of surgical removal of toxins

119.

When is inducing emesis generally contraindicated during toxin exposures?

a)

After ingestion of non-toxic table scraps

b)

After ingestion of caustic or jagged substances

c)

After ingestion of small, round, inert beads

d)

After ingestion of smooth plastic toys

120.

Which statement reflects the principle “dilution is the solution to pollution” in poisoned animals?

a)

Provide oxygen therapy to reduce absorption

b)

Use intravenous fluids to promote diuresis

c)

Administer corticosteroids to reduce swelling

d)

Give antibiotics to prevent aspiration

121.

What is the primary purpose of activated charcoal in toxin management?

a)

Neutralize gastric acid quickly and completely

b)

Stimulate diuresis to enhance renal excretion

c)

Bind toxins in the gut to limit absorption

d)

Induce vomiting to remove stomach contents

122.

A dog may have ingested a toxin but exposure is uncertain. Which action aligns with recommended principles?

a)

Treat as exposed to minimize potential harm

b)

Avoid all treatment to prevent side effects

c)

Wait for clear signs before any intervention

d)

Give hydrogen peroxide to induce vomiting

123.

Which toxin is primarily responsible for clinical signs after azalea or rhododendron ingestion in animals?

a)

Lycorine, a potent alkaloid

b)

Andromedotoxin, a plant glycoside

c)

Aconitine, a neurotoxic alkaloid

d)

Taxine, a cardiotoxic resin

124.

A 40 kg goat eats enough azalea leaves to reach 0.3% of its body weight. Which outcome is most likely within six hours?

a)

Hypersalivation with vomiting and depression

b)

Ataxia with seizures and blindness

c)

Petechiae with hematuria and anemia

d)

Dyspnea with cyanosis and collapse

125.

Cyclamen poisoning in small animals is most strongly associated with which plant part and consequence?

a)

Flower nibbling with photosensitivity

b)

Leaf chewing with mild lacrimation

c)

Stem intake with renal tubular necrosis

d)

Root ingestion with severe vomiting

126.

Which clinical profile best matches lycorine exposure from daffodils?

a)

Hematuria, polydipsia, azotemia, weight loss

b)

Ataxia, hyperesthesia, muscle tremors, mydriasis

c)

Bradycardia, miosis, bronchospasm, hypotension

d)

Severe vomiting, diarrhea, abdominal pain, drooling

127.

Across azalea, cyclamen, and daffodil toxicoses, which initial management approach is most consistently indicated?

a)

Supportive care tailored to clinical signs

b)

Immediate chelation with specific antidotes

c)

Alkalinization of urine to hasten excretion

d)

High-dose corticosteroids for antiemesis

128.

Which statement best distinguishes azalea toxicosis from daffodil toxicosis in animals?

a)

Azaleas rarely induce vomiting; daffodils rarely cause diarrhea

b)

Azaleas cause photosensitivity; daffodils primarily cause hepatotoxicity

c)

Azaleas need >0.2% body weight ingestion; daffodils cause emesis via lycorine

d)

Azaleas act within days; daffodils usually present after weeks

129.

Which clinical sign best characterizes the Dallis grass staggers form in ruminants?

a)

Severe diarrhea with bloody stools

b)

Inability to stand with muscle tremors

c)

Photosensitivity with skin crusting

d)

Profuse salivation with oral ulcers

130.

Dieffenbachia (Dumb Cane) causes oral irritation primarily due to which component?

a)

Cardiac glycosides

b)

Alkaloid nicotinic analogs

c)

Cyanogenic glycosides

d)

Calcium oxalate crystals

131.

A herd shows lameness, cold limbs, and tail tip sloughing after grazing a pasture. Which management step is most appropriate first?

a)

Begin high‑energy grain supplementation immediately

b)

Apply topical corticosteroids to affected limbs

c)

Remove animals from the source pasture promptly

d)

Administer broad‑spectrum antibiotics herd‑wide

132.

Puppies ingest English ivy berries. Which combination of signs is most expected?

a)

Ataxia, head pressing, blindness

b)

Bradycardia, hyperthermia, hematuria

c)

GI signs, labored breathing, convulsions

d)

Photosensitization, weight loss, anemia

133.

Which treatment pairing is most accurate for the listed plant toxicity?

a)

Dumb Cane—milk and antihistamines; Dallis grass—sedation

b)

Dallis grass—symptomatic only; English ivy—calcium therapy

c)

Dallis grass—broad antibiotics; English ivy—activated charcoal mandatory

d)

English ivy—milk and antihistamines; Dumb Cane—saline cathartics

134.

You are asked to reduce future Dallis grass toxicity on a farm. Which pasture action directly targets the toxic risk?

a)

Overseed with legumes each spring

b)

Bush‑hog the pasture to remove seedheads

c)

Apply nitrogen fertilizer heavily in summer

d)

Irrigate frequently to dilute plant toxins

135.

Which statement best describes why Johnson grass becomes dangerous after wilting?

a)

It accumulates oxalates in cool weather

b)

It concentrates nitrate during flowering periods

c)

It produces hepatotoxic pyrrolizidine alkaloids

d)

It harbors neurotoxic endophyte fungi

e)

It becomes cyanogenic and releases cyanide

136.

A herd of cattle develops dyspnea, bloat, pain, convulsions, and several deaths after grazing wilted Johnson grass. Which immediate therapy is most appropriate?

a)

Activated charcoal boluses for 3 days

b)

Magnesium sulfate drench once daily

c)

Sodium thiosulfate injections promptly

d)

Vitamin K1 injections for 5 days

e)

Atropine and pralidoxime infusion

137.

In small animals, ingestion of Kalanchoe most likely leads to which primary effect?

a)

Severe hepatic necrosis with icterus

b)

Cardiac arrhythmias with GI signs

c)

Renal tubular acidosis and PU/PD

d)

Acetylcholinesterase inhibition only

e)

Hemoglobinuria with methemoglobinemia

138.

All parts of Lantana, including berries, are toxic. What clinical picture is most characteristic in affected species such as sheep, cattle, horses, and even children?

a)

Profuse salivation with miosis

b)

Bloody watery diarrhea with paralysis

c)

Acute hemolysis with red urine

d)

Bradycardia with muscle tremors

e)

Progressive photosensitization only

139.

Ruminants are most susceptible to Johnson grass poisoning compared with other animals. Which reasoning best explains this higher risk?

a)

Ruminants lack hepatic cytochrome P450 systems

b)

Rumen motility prevents emesis after exposure

c)

Ruminants selectively ingest seed heads

d)

Rumen pH neutralizes detoxification enzymes

e)

Rumen microbes can rapidly liberate cyanide

140.

A dog chews Kalanchoe leaves and vomits repeatedly. Heart rhythm is irregular but blood pressure is stable. What is the most appropriate management plan?

a)

Immediate sodium thiosulfate injection

b)

Supportive care with rhythm monitoring

c)

High-dose corticosteroids for 7 days

d)

Alkalinize urine with sodium bicarbonate

e)

Chelation therapy with EDTA infusion

141.

Which component in false lilies like Peace and Calla primarily causes oral irritation in animals?

a)

Tropane alkaloids inducing anticholinergic effects

b)

Cardiac glycosides triggering bradyarrhythmias

c)

Alkaloids producing hepatic necrosis in dogs

d)

Calcium oxalate crystals causing mucosal irritation

142.

A cat ingests a small amount of pollen from an Easter lily. What is the most likely life‑threatening outcome without rapid intervention?

a)

Neurotoxicity with paralysis and coma

b)

Severe hemolysis with methemoglobinemia

c)

Fulminant hepatic failure with icterus

d)

Acute renal failure progressing to uremia

143.

Which immediate management plan best improves survival after true lily ingestion in cats?

a)

Induce emesis, give activated charcoal, start fluid diuresis

b)

Administer atropine, initiate lipid emulsion, restrict fluids

c)

Provide vitamin K, whole blood transfusion, oxygen therapy

d)

Begin broad‑spectrum antibiotics, give potassium supplementation

144.

Lily of the Valley poisoning is most directly associated with which pathophysiologic effect?

a)

Neurotoxic terpenes causing tremors and mydriasis

b)

Nephrotoxic oxalates causing crystalluria and colic

c)

Hepatotoxic saponins causing icterus and coagulopathy

d)

Cardiac glycosides causing arrhythmias and seizures

145.

During winter holidays, a dog eats mistletoe berries and develops vomiting and diarrhea. What is the most appropriate initial treatment priority?

a)

Provide symptomatic care with fluids and monitoring

b)

Administer nephroprotective dopamine infusion immediately

c)

Start digoxin‑specific Fab fragments empirically

d)

Initiate chelation therapy with EDTA promptly

146.

Which scenario best differentiates false lilies from true lilies in expected clinical signs?

a)

True lilies primarily induce hemolytic anemia in dogs

b)

False lilies cause fatal hepatic necrosis in cats

c)

True lilies cause only mild gastrointestinal upset

d)

False lilies cause local oral irritation with drooling

147.

Which plant exposure is most associated with acute renal failure in grazing animals?

a)

Red Maple wilted leaf exposure

b)

Prunus wilted leaf consumption

c)

Pigweed ingestion on poor pasture

d)

Oak acorn ingestion in autumn

148.

A ruminant presents with dyspnea, bloat, pain, convulsions, and rapid death after eating wilted leaves from a cherry tree. What is the primary toxin?

a)

Hydrogen cyanide produced in leaves

b)

Tremetol concentrated in seeds

c)

Oxalates released from stems

d)

Tannins causing gastrointestinal damage

149.

Which clinical picture best matches Red Maple toxicosis in horses?

a)

Hemolysis with icterus and hemoglobinuria

b)

Rear limb paralysis with dilute urine

c)

Primary bloat without blood changes

d)

Neurologic tremors with seizures only

150.

Symptoms from pigweed ingestion typically appear after which timeframe?

a)

One to three days post ingestion

b)

Two to four weeks after exposure

c)

Five to ten days post ingestion

d)

Within thirty minutes of exposure