WorksheetsMICRO-12
Total questions: 75
Worksheet time: 38mins
Name
Class
Date
1.
Bacitracin is susceptible if
a)
>10 mm ZOI
b)
>16 mm ZOI
c)
> 14 mm ZOI
d)
> 21 mm ZOI
2.
Optochin is susceptible if
a)
>10 mm ZOI
b)
>16 mm ZOI
c)
> 14 mm ZOI
d)
> 21 mm ZOI
3.
What is the positive result in PYR test?
a)
Deep purple
b)
Red
c)
Yellow
d)
Growth + yellow color
e)
Bright Red
4.
What is the positive result in Hippurate hydrolysis test?
a)
Deep purple
b)
Red
c)
Yellow
d)
Growth + yellow color
e)
Bright Red
5.
What is the positive result in LAP test?
a)
Deep purple
b)
Red
c)
Yellow
d)
Growth + yellow color
e)
Bright Red
6.
What is the positive result in Pyruvate test?
a)
Deep purple
b)
Red
c)
Yellow
d)
Growth + yellow color
e)
Bright Red
7.
What is the positive result in Salt tolerance test?
a)
Deep purple
b)
Red
c)
Yellow
d)
Growth + yellow color
e)
Bright Red
8.
Which test shows disintegration of colonies or clearing of broth culture when positive?
a)
CAMP test
b)
Bile solubility test
c)
Bile esculin hydrolysis
d)
Optochin test
9.
Which test shows arrowhead hemolysis when positive?
a)
CAMP test
b)
Bile solubility test
c)
Bile esculin hydrolysis
d)
Optochin test
10.
PYR test detects which enzyme?
a)
Hippuricase
b)
L-pyrrolidonyl arylamidase
c)
Leucine aminopeptidase
d)
Pyronidazole aminopeptidase
11.
A bright red PYR result can be observed after how many minutes?
a)
1 minute
b)
2 minutes
c)
3 minutes
d)
4 minutes
e)
5 minutes
12.
In the bile esculin test, blackening of the medium indicates hydrolysis of esculin in the presence of:
a)
40% bile
b)
40% esculin
c)
40% H2O2
d)
40% NaCl
13.
In the bile solubility test, S. pneumoniae is positive because it is capable of producing what enzyme through bile salt? This enzyme can rapidly auto-lyse its own colonies. :
a)
Catalase enzyme
b)
Amidase enzyme
c)
Beta-lactamase enzyme
d)
Bile enzyme
14.
Aclear broth after adding 2% sodium deoxycholate indicates:
a)
Bile Esculin hydrolysis positive
b)
Bile solubility positive
c)
PYR negative
d)
Optochin resistant
15.
Bile solubility test using colonies on BAP uses which reagent?
a)
2% Sodium deoxycholate
b)
10% Sodium deoxycholate
c)
Cinnamaldehyde
d)
0.2 mL ninhydrin reagent
16.
Bile solubility test using colonies on broth uses which reagent?
a)
2% Sodium deoxycholate
b)
10% Sodium deoxycholate
c)
Cinnamaldehyde
d)
0.2 mL ninhydrin reagent
17.
LAP test uses which reagent?
a)
2% Sodium deoxycholate
b)
10% Sodium deoxycholate
c)
Cinnamaldehyde
d)
0.2 mL ninhydrin reagent
18.
Hippurate hydrolysis test uses which reagent?
a)
0.2% Sodium deoxycholate
b)
0.2 g ninhydrin reagent
c)
0.2 uL ninhydrin reagent
d)
0.2 mL ninhydrin reagent
19.
How long would you wait upon applying Cinnamaldehyde reagent for LAP test?
a)
1 minute
b)
2 minutes
c)
3 minutes
d)
4 minutes
20.
How long would you incubate when performing Hippurate hydrolysis?
a)
10 to 30 minutes
b)
15 to 30 minutes
c)
25 to 30 minutes
d)
35 to 40 minutes
21.
What is the reagent or indicator in salt tolerance test?
a)
N,N-methylamino Cinnamaldehyde
b)
Ferric ammonium citrate
c)
Bromocresol purple
d)
Bromothymol blue
22.
What is the reagent or indicator in Pyruvate test?
a)
N,N-methylamino Cinnamaldehyde
b)
Ferric ammonium citrate
c)
Bromocresol purple
d)
Bromothymol blue
23.
What is the reagent or indicator in Bile Esculin hydrolysis test?
a)
N,N-methylamino Cinnamaldehyde
b)
Ferric ammonium citrate
c)
Bromocresol purple
d)
Bromothymol blue
24.
What is the reagent or indicator in PYR test?
a)
N,N-methylamino Cinnamaldehyde
b)
Ferric ammonium citrate
c)
Bromocresol purple
d)
Bromothymol blue
25.
Which tests shows growth and black precipitate when positive?
a)
Bacitracin susceptibility
b)
PYR test
c)
Bile esculin Hydrolysis
d)
Optochin (P disk) susceptibility
26.
What is the negative result in Pyruvate test?
a)
No color change
b)
No ZOI
c)
Colorless / yellow pink
d)
No color change / Yellow
e)
No color change or Yellow-green/Weak
27.
What is the negative result in LAP test?
a)
No color change
b)
No ZOI
c)
Colorless / yellow pink
d)
No color change / Yellow
e)
No color change or Yellow-green/Weak
28.
What is the negative result in Hippurate hydrolysis test?
a)
No color change
b)
No ZOI
c)
Colorless / yellow pink
d)
No color change / Yellow
e)
No color change or Yellow-green/Weak
29.
What is the negative result in Bacitracin susceptibility test?
a)
No color change
b)
No ZOI
c)
Colorless / yellow pink
d)
No color change / Yellow
e)
No color change or Yellow-green/Weak
30.
What is the negative result in PYR test?
a)
No color change
b)
No ZOI
c)
Colorless / yellow pink
d)
No color change / Yellow
e)
No color change or Yellow-green/Weak
31.
What is the negative result in salt tolerance test?
a)
Intact or insoluble colony
b)
No enhance or arrowhead hemolysis
c)
Little or no growth
32.
What is the negative result in CAMP test?
a)
Intact or insoluble colony
b)
No enhance or arrowhead hemolysis
c)
Little or no growth
33.
What is the negative result in bile solubility test?
a)
Intact or insoluble colony
b)
No enhance or arrowhead hemolysis
c)
Little or no growth
34.
What is the positive (QC) organism in bacitracin susceptibility?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
35.
What is the positive (QC) organism in PYR test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
36.
What is the positive (QC) organism in bile esculin hydrolysis?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
37.
What is the positive (QC) organism in optochin (P disk) susceptibility test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
38.
What is the positive (QC) organism in bile solubility test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
39.
What is the positive (QC) organism in CAMP test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
40.
What is the positive (QC) organism in LAP test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
41.
What is the positive (QC) organism in pyruvate test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
42.
What is the positive (QC) organism in salt tolerance test?
a)
S. pyogenes, M. luteus
b)
S. pyogenes, E. faecalis
c)
E. faecalis only
d)
S. pneumoniae
e)
S. agalactiae
43.
What is the negative (QC) organism in Bacitracin susceptibility?
a)
S. agalactiae, S. aureus
b)
S. pyogenes
c)
E. faecalis, viridans
d)
S. gallolyticus
44.
What is the negative (QC) organism in Optochin (P) disk susceptibility test?
a)
S. agalactiae, S. aureus
b)
S. pyogenes
c)
E. faecalis, viridans
d)
S. gallolyticus
45.
What is the negative (QC) organism in Bile solubility test?
a)
S. agalactiae, S. aureus
b)
S. pyogenes
c)
E. faecalis, viridans
d)
S. gallolyticus
46.
What is the negative (QC) organism in CAMP test?
a)
S. agalactiae, S. aureus
b)
S. pyogenes
c)
E. faecalis, viridans
d)
S. gallolyticus
47.
What is the negative (QC) organism in Hippurate hydrolysis test?
a)
S. agalactiae, S. aureus
b)
S. pyogenes
c)
E. faecalis, viridans
d)
S. gallolyticus
48.
What is the negative (QC) organism in PYR test?
a)
S. gallolyticus
b)
S. agalactiae only
c)
E. coli
d)
Aerococcus, viridans
e)
S. pyogenes
49.
What is the negative (QC) organism in LAP test?
a)
S. gallolyticus
b)
S. agalactiae only
c)
E. coli
d)
Aerococcus, viridans
e)
S. pyogenes
50.
What is the negative (QC) organism in pyruvate test?
a)
S. gallolyticus
b)
S. agalactiae only
c)
E. coli
d)
Aerococcus, viridans
e)
S. pyogenes
51.
What is the negative (QC) organism in Salt tolerance test?
a)
S. gallolyticus
b)
S. agalactiae only
c)
E. coli
d)
Aerococcus, viridans
e)
S. pyogenes
52.
What is the negative (QC) organism in bile esculin hydrolysis that shows growth but NO blackening?
a)
S. gallolyticus
b)
S. agalactiae only
c)
E. coli
d)
Aerococcus, viridans
e)
S. pyogenes
53.
What is the negative (QC) organism in bile esculin hydrolysis that doesn't exhibit any growth and blackening?
a)
S. gallolyticus
b)
S. agalactiae only
c)
E. coli
d)
Aerococcus, viridans
e)
S. pyogenes
54.
S. pyogenes
a)
Susceptible in Bacitracin
b)
Resistant in Bacitracin
55.
Micrococcus luteus
a)
Susceptible in Bacitracin
b)
Resistant in Bacitracin
56.
S. agalactiae
a)
Susceptible in Bacitracin
b)
Resistant in Bacitracin
57.
S. aureus
a)
Susceptible in Bacitracin
b)
Resistant in Bacitracin
58.
E. faecalis
a)
Positive PYR
b)
Negative PYR
59.
S. pyogenes
a)
Positive PYR
b)
Negative PYR
60.
S. agalactiae
a)
Positive PYR
b)
Negative PYR
61.
E. faecalis
a)
Positive Bile Esculin Hydrolysis
b)
Negative Bile Esculin Hydrolysis
62.
E. coli, S. pyogenes
a)
Positive Bile Esculin Hydrolysis
b)
Negative Bile Esculin Hydrolysis
63.
S. pneumoniae
a)
Susceptible in Optochin
b)
Resistant in Optochin
64.
S. pyogenes
a)
Susceptible in Optochin
b)
Resistant in Optochin
65.
S. pneumoniae
a)
Positive in Bile Solubility test
b)
Negative in Bile Solubility test
66.
E. faecalis
a)
Positive in Bile Solubility test
b)
Negative in Bile Solubility test
67.
Viridans
a)
Positive in Bile Solubility test
b)
Negative in Bile Solubility test
68.
S. agalactiae
a)
CAMP test Positive
b)
CAMP test Negative
69.
S. pyogenes
a)
CAMP test Positive
b)
CAMP test Negative
70.
E. faecalis
a)
LAP test Positive
b)
LAP test Negative
71.
Aerococcus viridans
a)
LAP test Positive
b)
LAP test Negative
72.
E. faecalis
a)
Pyruvate test Positive
b)
Pyruvate test negative
73.
S. gallolyticus
a)
Pyruvate test Positive
b)
Pyruvate test negative
74.
E. faecalis
a)
Salt tolerance test positive
b)
Salt tolerance test negative
75.
S. gallolyticus
a)
Salt tolerance test positive
b)
Salt tolerance test negative
100 %
