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LS.Bio.1.2 - Enzymes

Total questions: 24

Worksheet time: 19mins

Name
Class
Date
1.

What are enzymes?

a)

Proteins that speed up reactions by lowering activation energy

b)

Lipids that store energy for reactions

c)

Sugars that are consumed during reactions

d)

Nucleic acids that provide activation energy

2.

Which statement about how enzymes are used in reactions is correct?

a)

They are not consumed (reusable) and are substrate-specific

b)

They are consumed and work on any molecule

c)

They are reused only once per reaction

d)

They change substrates into enzymes after use

3.

How does temperature typically affect enzyme activity?

a)

High heat can denature enzymes, while low temperature slows reactions

b)

Low temperature denatures enzymes, while high heat speeds reactions without limit

c)

Both high heat and low temperature increase reaction rate indefinitely

d)

Temperature has no effect on enzymes

4.

What happens when an enzyme experiences a pH outside its optimal range?

a)

Its shape changes (denatures) and activity decreases

b)

Its shape remains unchanged and activity increases

c)

It produces more substrates

d)

It becomes permanently more efficient

5.

As substrate concentration increases, what typically happens to the reaction rate?

a)

It increases until reaching a maximum due to saturation

b)

It decreases steadily to zero

c)

It remains constant regardless of concentration

d)

It fluctuates randomly

6.

What best describes enzyme denaturing?

a)

The shape of the active site changes and the enzyme stops working

b)

The enzyme gains additional active sites and works faster

c)

The enzyme dissolves into substrates

d)

The enzyme temporarily pauses and then resumes normal activity

7.

In an investigation of enzyme reaction rate, which factor would be appropriate to vary to test its effect while keeping other conditions controlled?

a)

Temperature

b)

Amount of light

c)

Color of the solution

d)

Container shape

8.
Which factor would have the least effect on the rate at which an enzyme breaks down starch?
a)
 the pH of the solution in which the reaction is occurring
b)
the concentrations of starch and enzyme
c)
the temperature at which the reaction is taking place
d)
 the wavelength of light illuminating the reaction
9.

Which best explains why the rate of a biochemical reaction decreases at high temperatures?

a)

The activation energy increases.

b)

The shape of the enzyme changes.

c)

The enzyme-substrate complex forms.

d)

The bonds between substrates are broken.

10.

A student is investigating how substrate concentration affects the rate of an enzyme-catalyzed reaction.

Which three variables should be kept constant in the setup of the student’s investigation?

a)

temperature of the reaction

b)

amount of enzyme

c)

amount of substrate

d)

amount of product

e)

pH of the reaction

11.

Which color line represent a reaction with an enzyme present?

a)

Blue

b)

Red

c)

Both

d)

Neither

12.
Enzymes act as catalysts in biochemical reactions. Which is the enzyme?
a)
w
b)
x
c)
y
d)
wx
13.

Which of the following would most likely decrease the activity of an enzyme?

a)

Maintaining the pH at the enzyme's optimum

b)

Increasing the temperature beyond the enzyme's optimal range

c)

Adding more substrate to the reaction

d)

Using a buffer to stabilize pH

14.

What is the role of the active site in an enzyme?

a)

It produces new enzymes

b)

It transports molecules across membranes

c)

It stores energy for the cell

d)

It binds to specific substrates and facilitates the reaction

15.

Which statement best describes how enzymes affect activation energy?

a)

Enzymes do not change the activation energy

b)

Enzymes eliminate the need for activation energy

c)

Enzymes lower the activation energy required for a reaction

d)

Enzymes increase the activation energy needed for a reaction

16.

What would most likely happen to an enzyme if it is exposed to extremely acidic conditions?

a)

It would denature and lose its function

b)

It would become more efficient

c)

It would double in size

d)

It would convert into a substrate

17.

Which statement best describes the specificity of enzymes?

a)

Each enzyme acts on a specific substrate due to the shape of its active site

b)

All enzymes perform the same reaction

c)

Enzymes can act on any molecule in the cell

d)

Enzymes are only specific to temperature, not substrates

18.

What is the effect of increasing enzyme concentration when substrate is limited?

a)

The enzyme is consumed in the reaction

b)

The reaction rate remains unchanged

c)

The reaction rate decreases steadily

d)

The reaction rate increases and then levels off

19.

Based on these data, this enzyme functions best at what temperature and pH?

a)

Temperature of 27°C and a pH of 4

b)

Temperature of 37°C and a pH of 6

c)

Temperature of 40°C and a pH of 8

d)

Temperature of 50°C and a pH of 10

20.

Meat tenderizer contains an enzyme that interacts with meat. If meat is coated with the tenderizer and then placed in a refrigerator for a short time, how would the enzyme be affected?

a)

It would get broken down

b)

Its activity would slow down

c)

Its shape would change

d)

It would no longer act as an enzyme

21.
Sucrase has an optimum temperature of 37°C and an optimum pH of 6.2. What would happen if the temperature was changed to 70°C?
a)
Speed up the reaction
b)
No change in reaction
c)
Denature the protein
d)
Slow the reaction down
22.
Based on the graph, what is the optimal temperature for this enzyme? 
a)
15oC
b)
40oC
c)
30oC
d)
35oC
23.
Which of the following conclusions can be drawn from this graph?
a)
The optimum pH of the enzyme is 6.6.
b)
The optimum pH of the enzyme is 5.8
c)
The enzyme’s activity increases as pH increases 5.0 to 9.0
d)
The enzyme’s activity is greater around pH of 8.0 .
24.
Which enzyme is most efficient in a basic solution?
a)
A
b)
B