WorksheetsBiochemistry
Total questions: 25
Worksheet time: 13mins
What is the primary aim of the GOD/POD method described?
To estimate the amount of protein in a serum sample.
To estimate the amount of glucose present in a serum/plasma sample.
To measure the concentration of cholesterol in plasma.
Which enzyme oxidizes -D-glucose to gluconic acid and liberates hydrogen peroxide?
Peroxidase (POD)
Lipoprotein lipase
Glucose oxidase (GOD)
In the GOD/POD principle, the intensity of the final color is directly proportional to the concentration of which substance?
H2O2
Quinoneimine dye
Glucose in plasma
The specificity of the GOD/POD method for -D-glucose means cross-reaction with which substances does not pose an issue?
Other sugars
Lipids
Proteins
What is the concentration of the Glucose Standard used in the procedure?
100 mg/dL
200 mg/dL
0.7mmol/dL
The absorbance of the standard and test samples in the GOD/POD method is measured against which component?
Standard (S)
Test (T)
Blank (B)
At what wavelength (or filter color) is the absorbance of the GOD/POD reaction typically read?
505 nm
600 nm
400 nm
What is the normal range for Fasting Blood Glucose?
110-140 mg/dL
70-110 mg/dL
Less than 70 mg/dL
What is the aim of the triglyceride experiment?
To estimate the amount of glucose in serum.
To estimate the amount of triglycerides present in the given serum sample.
To estimate the total cholesterol.
Which enzyme hydrolyzes triglycerides to release glycerol in the first step of the triglyceride estimation principle?
Glycerol kinase
Glycerol phosphate oxidase
Lipoprotein lipase
The final colored compound in the triglyceride estimation is a red compound. What is required, along with hydrogen peroxide, to produce this colored compound?
Glucose oxidase
Peroxidase and phenolic chromogen
Glycerol kinase
What is the concentration of the Triglycerides standard mentioned in the reagents?
100 mg/dL
200 mg/dL
150 mg/dL
Which type of plasma/serum is mentioned as a suitable sample for triglyceride estimation?
Unhaemolysed serum
Haemolysed plasma
Plasma
What is considered a normal level of Ketone bodies in the blood?
Less than 125 mg/24 hr
Less than 3 mg/dL
Less than 3 mg/dL
What is the normal range for Hemoglobin levels in Women (NHP India)?
13.8-17.2 mg/dL
12-15 mg/dL
Less than 12 mg/dL
What is Ketosis?
An elevation of glucose levels in the body.
An elevation of ketone levels in the body.
A low concentration of triglycerides in the blood.
Ketone production is increased when a person follows which type of diet?
High-protein diet
Very low carb diet
High-fat, high-sugar diet
When the body does not have enough carbohydrates for energy, it burns fat and which other substance for fuel?
Glucose
Ketones
H2O2
Which of the following is listed as a symptom of ketosis?
Weight gain
Fruity odour in breath
Decreased ketones in the blood
After mixing the reagents for triglyceride estimation, for how long are the tubes incubated at 37 C?
30 minutes
10 minutes
2 hours
What is the normal level for Total cholesterol according to the document?
Less than 100mg/dL
Less than 200 mg/dL
40 mg/dL
What is the recommended normal level for LDL cholesterol?
Less than 100 mg/dL
Less than 200 mg/dL
40 mg/dL
What is the recommended normal level for HDL cholesterol?
Less than 100 mg/dL
Less than 40 mg/dL
40mg/dl or higher
What is the incubation temperature for the test tubes (Blank, Standard, Test) in the GOD/POD glucose estimation procedure?
25 °C (Room temperature)
37°C
50°C
The final colored compound formed in the GOD/POD method is a red quinoneimine dye. Which two components couple with nascent oxygen to form this dye?
Phenol and Gluconic acid
4-Aminoantipyrine and Phenol
Glucose and Water
