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Lecture 3

Total questions: 41

Worksheet time: 21mins

Name
Class
Date
1.

ATP stores energy in:

a)

Glycosidic bonds

b)

High-energy phosphate bonds

c)

Hydrogen bonds

d)

Peptide bonds

2.

Hydrolysis of ATP releases energy because:

a)

It increases entropy and releases Pi

b)

It consumes oxygen directly

c)

It oxidizes glucose

d)

It reduces NAD⁺

3.

NADH stores energy in the form of:

a)

High-energy phosphate bonds

b)

High-energy electrons

c)

Oxygen molecules

d)

CO₂

4.

Stepwise oxidation of glucose is advantageous compared to direct burning because:

a)

More CO₂ is released

b)

It captures energy efficiently in small steps

c)

It requires no enzymes

d)

It wastes less heat

5.

In cells, most ATP is produced by:

a)

Glycolysis only

b)

Substrate-level phosphorylation

c)

Oxidative phosphorylation (ETC + ATP synthase)

d)

Fermentation

6.

Glycolysis occurs in the:

a)

Cytosol

b)

Mitochondrial matrix

c)

Inner mitochondrial membrane

d)

Nucleus

7.

End products of glycolysis are:

a)

Acetyl-CoA + CO₂

b)

Pyruvate + ATP + NADH

c)

Lactate + CO₂

d)

GTP + citrate

8.

Substrate-level phosphorylation occurs in:

a)

Glycolysis and the citric acid cycle

b)

Electron transport chain

c)

ATP synthase

d)

Photosystem II

9.

Under anaerobic conditions, cells regenerate NAD⁺ by:

a)

Electron transport chain

b)

Fermentation

c)

ATP synthase reversal

d)

Fatty acid oxidation

10.

In human muscle, anaerobic glycolysis produces:

a)

Ethanol

b)

Lactate

c)

Acetyl-CoA

d)

Glycerol

11.

Acetyl-CoA enters the TCA cycle by combining with:

a)

Citrate

b)

Oxaloacetate

c)

Malate

d)

Succinate

12.

Each turn of the TCA cycle produces:

a)

1 ATP, 3 NADH, 1 FADH₂, 2 CO₂

b)

2 ATP, 2 NADH, 1 CO₂

c)

3 ATP, 2 FADH₂, 1 CO₂

d)

Only NADH

13.

The TCA cycle occurs in the:

a)

Cytosol

b)

Mitochondrial matrix

c)

Intermembrane space

d)

Inner membrane

14.

Most of the energy from glucose after TCA is stored in:

a)

ATP

b)

NADH and FADH₂

c)

CO₂

d)

Acetyl-CoA

15.

The oxygen consumed in cellular respiration is used to:

a)

Oxidize glucose directly

b)

Accept electrons at the end of the ETC

c)

Generate CO₂

d)

Hydrolyze ATP

16.

The ETC is located in the:

a)

Outer mitochondrial membrane

b)

Inner mitochondrial membrane

c)

Cytosol

d)

Matrix

17.

The ETC pumps protons into the:

a)

Mitochondrial matrix

b)

Intermembrane space

c)

Cytosol

d)

Nucleus

18.

The driving force for ATP synthesis is the:

a)

Na⁺ gradient

b)

Proton electrochemical gradient

c)

K⁺ gradient

d)

Membrane potential only

19.

ATP synthase produces ATP by:

a)

Substrate-level phosphorylation

b)

Using proton flow to drive conformational changes

c)

Direct electron transfer

d)

Hydrolyzing glucose

20.

Each NADH donates electrons sufficient to produce about:

a)

1 ATP

b)

2 ATP

c)

2.5–3 ATP

d)

5 ATP

21.

Mitochondria are dynamic and can:

a)

Change shape and number

b)

Divide and fuse

c)

Relocate in cells

d)

All of the above

22.

The mitochondrial outer membrane contains:

a)

Porins (non-selective channels)

b)

ATP synthase

c)

Citric acid cycle enzymes

d)

Cytochrome oxidase

23.

Which membrane is highly selective and hosts the ETC?

a)

Outer membrane

b)

Inner membrane

c)

Both equally

d)

None

24.

Proton pumping occurs at:

a)

Complex I, III, IV

b)

Complex II only

c)

ATP synthase

d)

Porins

25.

Final electron acceptor in mitochondrial ETC is:

a)

NAD⁺

b)

O₂

c)

H₂O

d)

Cytochrome c

26.

Chloroplasts generate ATP by:

a)

Substrate-level phosphorylation

b)

Chemiosmosis using light-driven ETC

c)

NADH oxidation

d)

Glycolysis

27.

Oxygen in photosynthesis is generated by:

a)

Water-splitting complex at Photosystem II

b)

Calvin cycle

c)

NADPH oxidation

d)

ATP synthase

28.

Sugars produced in chloroplasts can be:

a)

Stored as starch

b)

Used for ATP production

c)

Converted to other biomolecules

d)

All of the above

29.

Chemiosmotic coupling is considered:

a)

Unique to animals

b)

Ancient and conserved

c)

Exclusive to mitochondria

d)

A new evolutionary trait

30.

Both mitochondria and chloroplasts evolved from:

a)

Archaea

b)

Endosymbiotic bacteria

c)

Viruses

d)

Cyanobacteriophages

31.

Which carrier transfers electrons within the lipid bilayer?

a)

NADH

b)

Quinones

c)

Cytochrome c

d)

ATP

32.

The iron in heme serves as:

a)

Proton carrier

b)

Electron acceptor/donor

c)

ATP synthase rotor

d)

Structural protein

33.

Cytochrome c oxidase reduces O₂ to:

a)

O₂⁻ radical

b)

H₂O

c)

CO₂

d)

NADH

34.

Why is proton (H⁺) chosen for chemiosmosis instead of Na⁺/K⁺?

a)

Small size and abundance in water

b)

Higher redox potential

c)

Easier to pump

d)

All of the above

35.

Approximate ATP yield per glucose in eukaryotes is:

a)

10

b)

30–32

c)

50

d)

100

36.

If NADH transferred electrons directly to O₂:

a)

Energy would be lost as heat

b)

Proton pumping efficiency would increase

c)

More ATP would be made

d)

Nothing would change

37.

Which storage form provides long-term energy reserve IN ANIMAL ?

a)

Glycogen

b)

Fat

c)

Starch

d)

ATP

38.

Which storage form provides long-term energy reserve IN PLANT?

a)

Glycogen

b)

Fat

c)

Starch

d)

ATP

39.

Which storage form provides rapid mobilization during fasting?

a)

Fat

b)

Glycogen

c)

Protein

d)

Nucleic acids

40.

Why can animals convert sugar to fat but not fat to sugar?

a)

Acetyl-CoA cannot be converted back to pyruvate

b)

No gluconeogenesis from fatty acids

c)

Lack of necessary enzymes

d)

All of the above

41.

Which disease type is most associated with mitochondrial dysfunction?

a)

Cancer

b)

Neurodegenerative and metabolic diseases

c)

Infectious diseases

d)

Allergies