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Isolation of DNA and RNA, Blotting Techniques

Total questions: 40

Worksheet time: 30mins

Name
Class
Date
1.

The first step in DNA isolation is

a)

Purification

b)

Cell membrane rupture

c)

DNA precipitation

d)

Quantification

2.

EDTA is used in DNA isolation mainly to

a)

Precipitate DNA

b)

Degrade RNA

c)

Chelate metal ions and inhibit DNases

d)

Lyse nucleus

3.

Lysozyme helps in DNA isolation by

a)

Breaking hydrogen bonds

b)

Degrading RNA

c)

Breaking peptidoglycan layer

d)

Denaturing proteins

4.

SDS is used in DNA isolation to

a)

Digest DNA

b)

Remove lipids and denature proteins

c)

Stabilize DNA

d)

Precipitate salts

5.

Which enzyme removes RNA during DNA isolation?

a)

DNase

b)

Protease

c)

RNase

d)

Lysozyme

6.

Phenol-chloroform extraction is used to remove

a)

DNA

b)

RNA

c)

Proteins

d)

Salts

7.

DNA carries which type of charge?

a)

Positive

b)

Neutral

c)

Negative

d)

Variable

8.

Ion-exchange chromatography separates DNA based on

a)

Size

b)

Shape

c)

Charge

9.

The property of DNA that is exploited during agarose gel electrophoresis is

a)

Size

b)

Shape

c)

Charge

d)

Solubility

10.

Ethanol precipitation of DNA requires presence of

a)

Proteins

b)

RNase

c)

Salts like sodium acetate

d)

Detergents

11.

Plant DNA isolation is difficult due to presence of

a)

Nucleus

b)

Cell wall

c)

Ribosomes

d)

Vacuole

12.

mRNA isolation is mainly required for

a)

Genomic library construction

b)

Protein purification

c)

cDNA synthesis

d)

DNA replication

13.

Eukaryotic mRNA contains a special structure called

a)

Cap only

b)

Poly-A tail only

c)

Cap and poly-A tail

d)

Intron

14.

Poly-A tail is present at which end of mRNA?

a)

5′ end

b)

3′ end

c)

Middle

d)

Both ends

15.

Oligo-dT column is used to isolate

a)

rRNA

b)

tRNA

c)

mRNA

d)

DNA

16.

Oligo-dT binds to mRNA due to

a)

Hydrogen bonding

b)

Complementary base pairing

c)

Ionic interaction

d)

Covalent bonding

17.

rRNA and tRNA during oligo-dT chromatography

(a)  

18.

In column chromatography, molecules with high affinity for the stationary phase:

a)

Are degraded

b)

Flow through the column

c)

Precipitate

d)

Flow through the column

19.

Low-salt buffer is used to

a)

Bind mRNA

b)

Degrade RNA

c)

Elute mRNA

d)

Stabilize DNA

20.

Guanidinium thiocyanate is used to

a)

Stabilize proteins

b)

Denature RNases

c)

Precipitate DNA

d)

Bind RNA to column

21.

In organic extraction, RNA is found in

a)

Organic phase

b)

Interphase

c)

Upper aqueous phase

d)

Pellet

22.

Chloroform helps in mRNA isolation by

a)

Precipitating RNA

b)

Phase separation

c)

Degrading proteins

d)

Binding mRNA

23.

Blotting technique is used to detect:

a)

Lipids

b)

Carbohydrates

c)

DNA, RNA and proteins

d)

Vitamins

24.

Southern blotting is mainly used to detect:

a)

Proteins

b)

mRNA

c)

DNA fragments

d)

Lipids

25.

Northern blotting is used for the separation of:

a)

DNA

b)

mRNA

26.

Western blotting detects:

a)

DNA

b)

RNA

c)

Protein

d)

Carbohydrates

27.

Southern blotting technique was devised by:

a)

Watson

b)

Crick

c)

Southern

d)

Towbin

28.

The year in which Southern blotting was introduced is:

a)

1969

b)

1975

c)

1979

d)

1985

29.

Western blotting was devised by Towbin in:

a)

1975

b)

1977

c)

1979

d)

1981

30.

Western blotting is based on the principle of:

a)

DNA–DNA hybridization

b)

RNA–DNA hybridization

c)

Antigen–antibody reaction

d)

Enzyme–substrate reaction

31.

The membrane commonly used in blotting techniques is:

a)

Nylon membrane

b)

Agarose gel

c)

Nitrocellulose membrane

d)

Polyacrylamide gel

32.

In Southern blotting, DNA fragments are separated based on:

a)

Shape

b)

Charge

c)

Size

d)

Base composition

33.

Hybridized regions in Southern blotting are detected using:

a)

UV spectrophotometer

b)

X-ray film

c)

Microscope

d)

ELISA reader

34.

Dark bands on X-ray film indicate:

a)

Non-recombinants

b)

Contamination

c)

Recombinants

d)

Degraded DNA

35.

Southern blotting is useful in diagnosing:

a)

Viral infections

b)

Genetic defects

c)

Bacterial infections

d)

All of the above

36.

RFLP mapping is associated with:

a)

Northern blotting

b)

Western blotting

c)

Southern blotting

d)

ELISA

37.

SDS used in Western blotting stands for:

a)

Sodium dodecyl sulphate

b)

Sodium diethyl sulphate

c)

Sulphur dodecyl salt

d)

Sodium dichloride sulphate

38.

Western blotting uses which gel for electrophoresis?

a)

Agarose gel

b)

Polyacrylamide gel

39.

Blocking in Western blotting prevents:

a)

Protein degradation

b)

Gel melting

c)

Non-specific antibody binding

d)

Protein denaturation

40.

Common blocking agents used in Western blotting are:

a)

Glucose and sucrose

b)

BSA or milk powder

c)

Agarose and gelatin

d)

DNA and RNA

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