WorksheetsSolution Preparation Calculations Worksheet
Total questions: 18
Worksheet time: 28mins
How much Tween 20 is needed to prepare 250 mL of 10% Tween 20 (v/v)?
Answer to one decimal place.
20.0 mL
25.0 mL
50.0 mL
75.0 mL
How much Tween 20 is needed to prepare 250 mL of 25% Tween 20 (v/v)?
Answer to one decimal place.
50.0 mL
62.5 mL
75.0 mL
100.0 mL
How much NaCl is needed to prepare 400 mL of 5% NaCl (w/v)?
Answer to one decimal place.
10.0 g
15.0 g
20.0g
25.0g
How much SDS is needed to prepare 350 mL of 10% SDS (w/v)?Answer to one decimal place.
30.0 g
35.0 g
40.0 g
45.0 g
How much BSA is needed to prepare 50 mL of 5 mg/mL of BSA?
Answer to zero decimal places.
125 mg
200 mg
250 mg
500 mg
How much tetracycline is needed to prepare 100 mL of 100 mg/mL tetracycline?
Answer to one decimal place.
1.0 g
5.0 g
10.0 g
12.0g
How much Ca(NO3)2 is needed to prepare 500 mL of 0.5 M Ca(NO3)2? MW = 164.09 g/mol
Answer to two decimal places
20.51 g
41.02 g
82.05 g
102.57 g
How much NaCl is needed to prepare 250 mL of 0.5 M NaCl? MW of NaCl = 58.44 g/mol
Answer to three decimal places
5.844 g
7.305 g
8.766 g
11.688 g
How much CaCl2 is needed to prepare 100 mL of 50 mM CaCl2? MW of CaCl2 = 110.98 g/mol
Answer to 3 decimal places
0.111 g
0.278 g
0.555 g
1.110 g
Using the dilution formula C1V1=C2V2 , determine the volumes of stock solution and water needed to prepare 100 mL of 2.5 X NaOH solution from 50 X NaOH solution. Choose the correct pair: Stock volume; Water volume.
Stock: 5 mL; Water: 95 mL
Stock: 2.5 mL; Water: 97.5 mL
Stock: 10 mL; Water: 90 mL
Stock: 20 mL; Water: 80 mL
If I have 340 mL of a 0.5 M NaBr solution, what will the concentration be if I add 560 mL more water to it?
.188 M
3.78 M
.389 M
1.76 M
In the equation, A = ebc, what quantity is represented by "e" ?
Molar Absorbtivity
Path Length
Concentration
Absorbance
Which of the following best describes Lambert-Beer’s Law in spectrophotometry?
The absorbance of a solution is directly proportional to the concentration of the absorbing species and the path length of the sample.
The transmittance of a solution increases as the concentration of the absorbing species increases.
The intensity of transmitted light is independent of the sample concentration and path length.
The absorbance of a solution remains constant regardless of changes in concentration or path length.
What is the technique used to separate molecules on the basis of size or density by high speed spinning?
Homogenization
Centrifugation
Filtration
Chromatography
What is the main purpose of filtration in protein purification?
To remove cellular debris
To concentrate the cells
To separate the protein from other molecules
To break the cells open
What is the main purpose of size exclusion chromatography?
To separate molecules based on size/shape
To separate molecules based on charge
To separate molecules based on hydrophobicity
To separate molecules based on affinity
What is the purpose of the wash buffer (WB) applied to the column after the supernatant?
The wash buffer is used to remove the green fluorescent protein from the column so that it can be collected.
The wash buffer is used to remove hydrophilic cellular proteins that are not bound to the beads.
The wash buffer is used to remove the hydrophobic cellular proteins that are bound to the beads.
