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WorksheetsVenipuncture and Tube Collection
Total questions: 20
Worksheet time: 10mins
What is the maximum depth for a heel stick on a newborn?
1.0mm
1.5mm
2.0mm
2.5mm
What is the proper technique for tube inversions?
Gently invert the tube completely (180 degrees), then return to upright position
Shake the tube vigorously for 10 seconds
Tilt the tube at a 45-degree angle and hold for 5 seconds
Invert the tube halfway and then shake it lightly
How long should a tourniquet remain on the patient's arm?
No more than 1 minute
No more than 5 minutes
No more than 10 minutes
No more than 30 minutes
What is the correct order to draw a therapeutic drug level (THA level), lipid panel, and CBC?
Red or gold (THA), lavender (CBC), red or gold (lipid panel)
Red or gold (lipid panel), red or gold (THA), lavender (CBC)
Lavender (CBC), red or gold (THA), red or gold (lipid panel)
Lavender (CBC), red or gold (lipid panel), red or gold (THA)
How many times can a specimen be centrifuged?
Only once
Twice
Three times
As many times as needed
What is the correct order of draw for capillary (dermal) puncture?
Serum tubes first, then EDTA tubes (lavender), then other additive tubes
EDTA tubes (lavender) first, then other additive tubes, then serum tubes last
Other additive tubes first, then serum tubes, then EDTA tubes (lavender)
Serum tubes first, then other additive tubes, then EDTA tubes (lavender)
What are the steps for drawing blood cultures?
Clean site with chlorhexidine or iodine in concentric circles from inside out, allow to dry, draw aerobic bottle first (or per facility protocol)
Draw blood without cleaning the site to save time
Use only alcohol wipes to clean the site before drawing blood
Draw anaerobic bottle first before the aerobic bottle
What is the difference between aerobic and anaerobic blood culture bottles?
Aerobic bottles contain oxygen for bacteria that require oxygen; anaerobic bottles do not contain oxygen for bacteria that grow without oxygen
Aerobic bottles are used for viruses; anaerobic bottles are used for bacteria
Aerobic bottles are for blood samples; anaerobic bottles are for urine samples
Aerobic bottles are used in high-altitude conditions; anaerobic bottles are used in low-altitude conditions
What should be done before placing specimens in a centrifuge?
Ensure specimens are balanced in the centrifuge, caps are secure, and inspect for cracks or defects
Place specimens directly into the centrifuge without any checks
Only check the temperature of the specimens
Ensure the centrifuge is turned on before placing specimens
What is the correct technique for collecting capillary blood into microtainers?
Apply gentle pressure to encourage blood flow, allowing drops to form naturally; touch collection device to the drop without scraping the skin
Use a vacuum tube to draw blood directly from the capillary
Scrape the skin to collect blood more efficiently
Apply excessive pressure to force blood out quickly
When should specimens be labeled?
At the patient's bedside immediately after collection
Before the specimen is collected
After the specimen is processed in the lab
At the time of patient discharge
When collecting a specimen for drug testing, how soon should the temperature be checked?
Within 2 minutes
Within 4 minutes
Within 6 minutes
Within 10 minutes
What is a chain of custody and which tests require it?
A tracking document ensuring specimen integrity for legal purposes; required for drug testing, alcohol levels, and forensic specimens
A method for storing specimens without any legal implications
A process for analyzing specimens without documentation
A guideline for conducting tests without tracking the samples
What tests need to be kept cold or on ice during transport?
Complete blood count (CBC)
Arterial blood gases (ABGs), ammonia, lactic acid, pyruvate
Lipid panel
Thyroid function tests
Which tube top color contains no additive and is used for serum chemistry tests?
Red
Green
Blue
Yellow
What is the primary difference between serum and plasma?
Plasma contains anticoagulant and clotting factors; serum does not contain clotting factors
Serum contains more proteins than plasma
Plasma is derived from red blood cells; serum is not
Serum contains anticoagulant and clotting factors; plasma does not contain clotting factors
What should a phlebotomist do if a tube breaks during centrifugation?
Immediately remove the tube and discard it without waiting
Wait for the centrifuge to stop completely, allow aerosols to settle (10-15 minutes), wear PPE, and clean with appropriate disinfectant
Continue centrifugation until all tubes are finished
Notify the supervisor and leave the area without cleaning
Which tube top color is used for a Complete Blood Count (CBC)?
Red top
Green top
Lavender/purple top
Blue top
What is the maximum number of venipuncture attempts a phlebotomist should make on a single patient?
One attempt, then seek assistance from another phlebotomist
Two attempts, then seek assistance from another phlebotomist
Three attempts, then seek assistance from another phlebotomist
Four attempts, then seek assistance from another phlebotomist
What tests are included in newborn metabolic screening besides PKU?
Hypothyroidism, galactosemia, and sickle cell disease
Diabetes, cystic fibrosis, and hemophilia
Asthma, allergies, and tuberculosis
HIV, hepatitis, and syphilis
