NC EOC Review: DNA Fingerprinting & Gel Electrophoresis

Front

What is the role of restriction enzymes?

Back

To cut the DNA into fragments.

Front

What do restriction enzymes act like?

Back

Biochemical scissors that cut DNA.

Front

What are the pieces of DNA seen in a DNA fingerprint called?

Back

Fragments.

Front

How does gel electrophoresis separate DNA fragments?

Back

By size/weight.

Front

What is DNA fingerprinting used for?

Back

To identify individuals based on their unique DNA patterns.

Front

What is the purpose of gel in gel electrophoresis?

Back

To provide a medium for DNA fragments to move through.

Front

What charge do DNA fragments carry?

Back

Negative charge due to the phosphate backbone.

Front

What is the significance of the size of DNA fragments in gel electrophoresis?

Back

Smaller fragments move faster and farther than larger ones.

Front

What is the first step in DNA fingerprinting?

Back

Extraction of DNA from cells.

Front

What is the process of amplifying DNA called?

Back

Polymerase Chain Reaction (PCR).