PLTW Medical Interventions Unit 4

Front

What does SDS-PAGE do?

Back

Removes the charge of proteins.

Front

What is required for GFP to glow when inserted into a plasmid with ampicillin resistance and a T7 promoter?

Back

IPTG is required for GFP to glow.

Front

Where are hydrophobic amino acids typically located in a protein structure?

Back

In the middle of the protein structure.

Front

Which component of recombinant DNA acts as the electricity?

Back

IGTP.

Front

How many stages of end-stage renal disease are there?

Back

5.

Front

What is the function of a T7 promoter in recombinant DNA?

Back

It initiates transcription of the gene of interest.

Front

What role does ampicillin play in plasmid selection?

Back

It provides antibiotic resistance, allowing only transformed cells to grow.

Front

Define recombinant DNA.

Back

DNA that has been artificially made by combining DNA from different organisms.

Front

What is the significance of sticky ends in DNA cloning?

Back

They allow for the ligation of DNA fragments by providing complementary overhangs.

Front

What is the role of GFP in molecular biology?

Back

GFP is used as a marker to visualize gene expression.