PCR and Gel Electrophoresis

Front

What is the purpose of the DNA extraction solution used before PCR?

Back

To break open the cell and nuclear membranes.

Front

In gel electrophoresis, where will the largest DNA fragment appear?

Back

Closest to the starting wells.

Front

What is the primary purpose of PCR?

Back

To produce billions of copies of a specific gene.

Front

What does gel electrophoresis do?

Back

It separates DNA based on size.

Front

If a restriction enzyme makes one cut in the gene, how many bands will appear on the gel?

Back

2.

Front

What is PCR an abbreviation for?

Back

Polymerase Chain Reaction.

Front

What are the main components of a PCR reaction?

Back

DNA template, primers, DNA polymerase, and nucleotides.

Front

What role do primers play in PCR?

Back

Primers are short sequences of nucleotides that initiate DNA synthesis.

Front

What is the significance of gel electrophoresis in DNA analysis?

Back

It allows for the visualization and separation of DNA fragments.

Front

How does the size of DNA fragments affect their movement in gel electrophoresis?

Back

Smaller fragments move faster and farther than larger fragments.