Cell Culture and Polio Vaccines

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History of cell culturing in the development of polio vaccines

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- 1930: Used live animals to grow poliovirus - 1970: Development of mammalian cell culture techniques - Modern: Widely used in laboratory settings

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Definition of cell culture

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Removal of cells and growth in a favorable artificial environment. Cells may be removed directly from tissue or derived from an established cell line.

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Primary culture characteristics

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- Obtained directly from donor tissues - Only able to divide a finite number of times due to telomere loss - Refers to the stage after cells are isolated from tissue and proliferated under certain conditions

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Establishing cell lines

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- Able to grow indefinitely - Useful for long-term research - Obtained from clinical tumors or transformation of cells

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Passaging or sub cultivation of a primary culture

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Begins the process of proliferating a culture indefinitely with the right treatment, conditions, and space (Immortalisation).

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Cell growth environment: Monolayers

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Adherent culture

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Cell growth environment: Free-floating

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Suspension cultures

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Culture ware examples

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Flasks, Plates, Roller bottles

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Commercial media types

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DMEM, RMPI, F12

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Cell culture laboratory requirements

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Appropriate culture media, Correct temperature