Gel Electrophoresis & DNA Fingerprinting

Front

What is gel electrophoresis?

Back

A laboratory technique used to separate DNA fragments based on their size and charge.

Front

What is the charge of DNA?

Back

DNA is negatively charged due to its phosphate backbone.

Front

What role do restriction enzymes play in DNA analysis?

Back

They cut DNA into specific segments for analysis.

Front

Why do DNA fragments move in gel electrophoresis?

Back

They move towards the positive electrode because they are negatively charged.

Front

What is DNA fingerprinting?

Back

A technique used to identify individuals based on unique patterns in their DNA.

Front

What is the significance of DNA fingerprinting in forensics?

Back

It helps to identify suspects or victims by comparing DNA samples.

Front

What is agarose gel?

Back

A substance used to create a gel matrix for separating DNA fragments during electrophoresis.

Front

How does the size of DNA fragments affect their movement in gel electrophoresis?

Back

Smaller fragments move faster and further through the gel than larger fragments.

Front

What is the purpose of loading dye in gel electrophoresis?

Back

It helps to visualize the DNA samples and monitor their progress during electrophoresis.

Front

What is the difference between DNA and RNA?

Back

DNA contains deoxyribose sugar and is double-stranded, while RNA contains ribose sugar and is usually single-stranded.