Laboratory Safety and Bacteriological Techniques

Laboratory Safety and Bacteriological Techniques

by Sarah Johnson

• ​Aseptic Techniques are used to protect people work with potentially harmful organisms.

• ​​Procedures used to keep biological materials contained.

• ​Protects all the environment from exposure.

• ​Literally means "without infection...or without microorganisms

Multiple Choice

Procedures used to keep biological materials contained.

• Sterilizing

• Aseptic Technique

• Biological Cleaning

• Disinfecting

Multiple Choice

What techniques are used to protect the people who work with potentially harmful organisms?

• Aesptic

• Biological

• Chemical

• Organic

Multiple Choice

What should a person do if unsure of the dangers of a chemical material?

• Call a friend

• Refer to the MSDS

• Never use the chemical

​Importance of Aseptic Technique

• ​Workers are protected by protective equipment (e.g., lab coat, safety gloves, and safety glasses, safety goggles)

• ​Clean lab work area with:

• ​Disinfectant (harsh chemical that kills or prevents growth of microorganisms).

• ​Aerosols (clouds of liquid particles in the air that can escape when opened.)

• ​Sterilized (made free of microorganisms before use or disposal.)

• ​Laminar airflow hood (moves air through a filter capable of removing biological entities.)

Fill in the Blank

Name three types of Protective Equipment used in a biotechnology laboratory (hint: 'safety first').

Multiple Choice

A harsh chemical that kills or prevents the growth of microorganisms.

• Laminar Airflow Hood

• Sterile Cleaner

• Disinfectant

• Aerosols

Multiple Choice

Clouds of liquid particles in the air that can easily escape when you open containers.

• Aerosols

• Disinfectant

• Bt Spray

• Biological Entities

Multiple Choice

A device to move air through a filter capable of removing biological entities.

• Sterilization Chamber

• Airflow Hood

• The Hood

• Laminar Airflow Hood

​Handling Wastes

• ​Chemical materials are a regular use in biotech lab.

• ​All materials may be hazardous

• ​Best source of information for hazardous materials is an....

• ​MSDS (material safety data sheet) - lists any hazard of a given material.

​Handling Wastes

• ​Safe biological waste can be put into a regular garbage can for local collection.

• ​Preferable disposable method is to compost the material.

• ​That waste contains microbes that need to be destroyed before disposal.

• ​Large & Bulky = spray with disinfectant.

​Handling Wastes

• ​Personal Protective Equipment (PPE) equipment worn to protect the human body from injury.

• ​It is REQUIRED in lab; the following must be available.

• Goggles

• Safety Glasses

• Face Shield

• ​Earplugs

• ​Respirator

• ​Particle Mask

• ​Earplugs

​Handling Waste

• ​Chemical cartridge and air-purifying respirators are more expensive devices.

• ​They provide better protection from:

• fumes

• ​vapors

• ​other gases

Multiple Choice

Eye related PPE with lenses and rims that prevent solid objects, liquids, and other materials from getting into the eye

• Safety Goggles

• Face Shield

• Safety Glasses

• Face Guard

Multiple Choice

Type of mask that protects the user's entire face.

• Particle Mask

• Respirator

• Face Mask

• Face Shield

Multiple Choice

Equipment worn or used to protect the human body from injuiry.

• Safety Dress

• PPT

• Personal Protective Equipment

• Lab Attire

Multiple Choice

A device designed to protect the wearer from inhaling harmful dust, liquid droplets, fumes, vapors, and other materials in the air.

• Respirator

• Chemical Cartridge

• Disposable Mask

• Particle Mask

Multiple Choice

An inexpensive, single use, disposable mask that filters particulate matter; as the contaminated air passes through the filter material.

• Air-Purifying

• Respirator

• Disposable Mask

• Particle Mask

Multiple Choice

Sound absorptive devices that prevent the passage of sound waves into the ear.

• Earplugs

• Earmuffs

• Headphones

• Airpods

Multiple Choice

How must large, bulky biological material be treated before disposal?

• Rinsed with water

• Autoclave sterilization

• Disinfectant Spray

• Put into a container

Multiple Choice

To be effective, a particle mask should be clean and fit properly over your.....

• nose and mouth

• your entire face

• nose

• mouth

Multiple Choice

Specially designed spectacles that prevent flying objects from getting into the eyes

• Goggles

• Safety Glasses

• Face Shield

• Particle Mask

​Safe Lab Practices

1. ​Safety glasses are particularly important in situations where high temperatures and/or pressures above atmospheric or hazardous substances are being used.

2. ​Mix cultures or reagents in such a way as to avoid splashing.

3. ​NEVER pipette broth cultures by mouth.

​Safe Lab Practices

1. ​Cover spilled cultures with paper towels.

2. ​Saturate area with disinfectant solution.

3. ​Allow 15 minutes of reaction time.

4. ​Sterilize contaminated clothing, equipment, and cultures by autoclave at NO LESS than 121*C for 20 minutes.

Multiple Choice

What device sterilizes microbes before disposing of them?

• Autoclave

• Incenerator

• Centrifuge

• Garbage Disposal

Multiple Choice

How long must glassware be heated at 325* degrees F to be considered sterile?

• 20 minutes

• 30 minutes

• 1 hour

• 2 hours

​Safe Lab Practices

• ​Report every incident immediately to the supervisor in charge.

• ​The supervisor should then file a report describing the circumstances of the incident.

​Liquid Waste Disposal

• ​Materials poured into the drain run through a pipe.

• ​Waste water then goes to a treatment plant.

• ​Chemicals are added to remove some of its contents.

• ​Solid matter (sludge) is separated out.

• ​Drinking water could contain anything hazardous that passed into our water source.

Multiple Choice

What material is separated in a waste water treatment plant?

• organic matter

• biological microbes

• Sludge

• biochemicals

Multiple Choice

What gas is used to sterilize disposable materials?

• Helium

• Nitrogen

• Carbon Dioxide

• Ethylene Oxide

​Summary

​All waste from a biotechnology laboratory, whether solid or liquid, should be considered dangerous.

​

​It is essential to determine whether it is hazardous. If it is, the proper means must be used to make it nonhazardous. By way of.....

​

​- Professional hazardous materials handlers

Multiple Choice

A chance an accident will occur

• Carelessness

• Risk

• Accident

• Hazard

Multiple Choice

An event that occurs unintentionally

• Risk

• Carelessness

• Accident

• Hazard

Multiple Choice

A source of danger or risk

• Carelessness

• Risk

• Accident

• Hazard

Multiple Choice

A form sent with all purchased chemical material, listing any dangers of the material.

• Material Sheet

• Material Safety Data Sheet (MSDS)

• MSSD

• Data Sheet

Multiple Choice

Materials that can disintegrate the flesh

• Chemical Materials

• Biological Materials

• Corrosives

• Sulfuric Acid

Multiple Choice

A pressure vessel that heats steam above 212* (100*C) by increased pressure; sterilized by ethylene oxide gas.

• Laminar Airflow Hood

• Water Bath

• Autoclave

• HEPA

Multiple Choice

Filters the air before it reaches the work useful in keeping the cultures pure.

• Thermocycler

• Laminar Airflow Hood

• Autoclave

• HEPA

Multiple Choice

All materials must be sterilized/autoclaved before media disposal then washed.

• Yes

• No

• Maybe

• Sometimes

Multiple Choice

Materials that could burn or explode very easily. Must be isolated from Ethyl alcohol and flammable materials.

• Flammables

• Oxidizers

• Irritants

• Carcinogens

Multiple Choice

Materials suspected of causing or proven to cause cancer.

• Cultures

• Flammables

• Carcinogens

• Irritants

Multiple Choice

Materials that can cause a reaction from the skin, eyes and nasal membranes.

• Oxidizers

• Irritants

• Carcinogens

• Flammables

Multiple Choice

Filter that will remove particles larger than 0.3 $$\mu m$$  to prevent any potentially harmful exposure to the work environment.

• Microcentrifuge

• Autoclave

• High Energy Particle Air (HEPA)

• Laminar Airflow Hood

Multiple Choice

Who should be the first person identified when an incident in laboratory occurs?

• a physician

• the supervisor

• a coworker

• a friend