
Tissue culture
Presentation
•
Science
•
University
•
Hard
Abhila Sr
FREE Resource
10 Slides • 2 Questions
1
Tissue culture banana
2
•Pest and disease free at planting.
•Tissue culture vigour provides better growth
and better performance in the field.
•Available through out the year.
3
•Progenies are uniform and true-to-type.
•Uniform maturity and harvesting.
•Ratoon crop is a unique feature.
4
Mother plant
Sucker selected
Explant
Shooting
Multiple shooting
Rooting
Hardening
VIRUS
INDEXING
5
Multiple Choice
Recalcitrant seeds are:
Easy to dry and store
Includes seeds from tropical fruits
Also known as orthodox seeds
Germinates while on parent plant
6
Multiple Choice
Seed coat dormancy is:
Detected by embryo excision
Due to dead embryo
Due to presence of ABA
Overcome by cotyledon removal
7
Cultivation of TC banana
•2 ½ - 3 month old TC plants should be used for
planting.
•Prepare pits in advance.
•Apply FYM + Green manure @ 15-20 kg / pit and
mix with top soil.
•Apply 500g lime / pit in acidic soils.
8
9
TC 6 splits
Urea
Rajphos
Muriate of
Potash
1MAP
110
260
115
2MAP
110
115
3MAP
110
275
115
4MAP
110
115
5MAP
110
115
ABE
110
210
Compost 15 kg/Pit ,Lime 500g/pit
16;16;16 /17:17;17/119;19;19 @5g/litre
Shade 10 days
Nendran -Tissue culture
10
Nendran 6
splits
Urea
Mussourieph
os
Muriate of
potash
1MAP
90
350
100
2MAP
65
250
100
3MAP
65
00
100
4MAP
65
00
100
5MAP
65
00
100
ABE
65
Nendran suckers
11
Palayankodan 100:200:400 g
10 kg FYM
Urea
Rajphos
MOP
2MAP
108
277
334
4MAP
109
278
334
12
GrandNain, Red banana,Njalipoovan
180:180:360 NPK g/plant
Urea gram
Rajphos
gram
MOP gram
2MAP
195
500
300
4MAP
195
499
300
Tissue culture banana
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