Electrophoresis

Mr. Jones was killed in a car accident.  Afterwards, a former girlfriend filed a paternity suit claiming that Mr. Jones was the father of her child, and thus she was entitled to the man’s estate.  Which sample of DNA would have to be from Mr. Jones if he were the child’s father? 

• 2
• 3
• 4
• 5

A boy moves to another country to live with his father.  Years later, the boy decides to move back to live with the woman who raised him.  Immigration officials suspected that the boy was the woman’s nephew, not her son, and therefore not legally entitled to residency.  Based on the DNA fragments, is it possible that she is the boy’s mother?

• Yes
• No

You load some DNA into a gel with the following Restriction Fragment Length Polymorphism lengths—25bp, 13bp, 54bp, and 11bp. Which of these four would travel the greatest distance from the well during electrophoresis?

• 25bp
• 13bp
• 54bp
• 11bp

Put the following in order from 1st step to last step.
A. Stain the gel
B. PCR
C. Load the DNA
D. Analyze
E. Add restriction enzymes
F. Electrophorese

• B, E, C, F, A, D
• D, A, C, B, F, E
• F, C, A, E, B, D
• B, C, E, A, D, F

How do scientists cut DNA into smaller strands?

• restriction enzymes
• scissors
• ligase
• agarose

Why do you use a new pipette tip on the micropipette every time you get a new sample?

• To avoid contaminating the other samples
• To use as many tools as you can.
• The pipette tips disappear after being used.
• None of these reasons

Pattern of DNA fragments obtained by examining a person’s unique sequence of DNA base pairs

• Chromosome
• DNA Probe
• DNA Fingerprint
• Electrophoresis

In this technique, DNA is loaded via a micropipettor, electric current turned on, then travels down the agarose, is stained and x-rayed.

• capillary electrophoresis
• gel electrophoresis
• PCR
• extraction

Why does DNA move through an electrophoresis once the electricity is passed through it?

• it creates a vacuum
• like charges attract
• DNA is positively charged
• DNA is negatively charged

• results of gel electrophoresis
• results of capillary electrophoresis
• PCR results
• all of the above