HUMAN INSULIN PRODUCTION

How many polypeptide chains are present in an insulin precursor molecule?

• one

• two

• three

• four

Name the technique used by Eli Lilly company for the production of Human insulin.

• Recombinant DNA technology

• Polymerase Chain reaction

• Electrophoresis

• DNA Fingerprinting

Choose the correct sequence for the production of Human insulin:

a. Preparation of two DNA sequences corresponding to A

and B, chains of human insulin

b. Chains A and B were produced separately in E.coli,

extracted from E.coli.

c. Introduction of DNA strands in plasmids of E. coli to

produce insulin chains.

d. Joining of A and B polypeptide by disulfide bonds to

form human insulin.

• a, b, c, d

• a, c, b, d

• a, b, d, c

• a, d, b, c