Column Chromatography 1

It is a technique or method used to separate mixtures of dissolved chemicals/substances.

• chromatography

• solution

• chromatogram

• evaporation

In size exclusion chromatography, the higher the degree of cross-linking of the gel polymer, ………..

• the lower the mechanical strength and the larger the molecular sieving range.

• the higher the mechanical strength and the larger the molecular sieving range.

• the lower the mechanical strength and the smaller the molecular sieving range.

• the higher the mechanical strength and the smaller the molecular sieving range.

In gel permeation chromatography, a type of size exclusion C, which of the following is correct?

• Compressible gels are commonly used.

• Spherical bead shape is preferred.

• Mechanical strength increases with large particle size.

• Gels with large bead size are preferred.

Q. Chromatography is generally used for the separation of mixtures based on the differences in their

• shape

• size

• structure

• all of the above

Ion-exchange chromatography is used for the separation of

• polar molecules

• nonpolar molecules

• none of the above

• all of the above

Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?

• ion exchange chromatography

• affinity chromatography

• adsorption chromatography

• igel permeation chromatography

In gel filtration (size exclusions) chromatography, separation of proteins are based on their

• size and net charge

• size and shape

• size and specific affinity

• shape and net charge

Which polymer is often used for matrix materials in Affinity chromatography

• Agarose

• cellulose

• dextrose

• all of the above

In anion exchange chromatography,

• the column contains positively charged beads where negatively charged proteins bind

• the column contains negatively charged beads where positively charged proteins bind

• the column contains negatively & positively charged beads where proteins bind depending on their net charge

• all of the above