PCR and Gel Electrophoresis

What was the purpose of the DNA extraction solution (tube #1) we used before we did PCR?

• Break open the cell and nuclear membranes

• Put DNA back into cells so we can study it

• Increase the Amount of DNA

• Decrease the Amount of DNA

What is the purpose of PCR?

• To produce billions of copies of a specific GENE

• To cut DNA

• To remove DNA from the nucleus

What is the function of a primer in PCR?

• To identify the particular gene of DNA to be copied by PCR.

• To copy DNA.

• To remove DNA from the nucleus

How many copies of DNA are made after 1 cycle of PCR? (we did 30 cycles during the last block period).

• 1

• 2

• 1 million

• 4

In preparation for an electrophoresis procedure, restriction enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

What is the force that separates the DNA fragments by size during gel electrophroesis?

• Magnetic force

• Gravitational force

• Electrical force

• Jiu Jitsu

Tags

NGSS.HS-PS2-4

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

If a restriction enzyme makes one cut in the gene, how many bands will appear on the gel?

• 0

• 1

• 2