11th - pGLO Lab

Bacteria taking up new DNA that they were not originally "born" with is called

• Horizontal Gene Transfer

• Vertical Gene Transfer

• Forced Gene Transfer

• Gene Therapy

In this lab, we created conditions to force the bacteria to uptake surrounding DNA. This is called bacterial______________

• Conjugation

• Transformation

• Transduction

• Translocation

Before bacteria can uptake DNA, you have to

• Mentally prepare the E. Coli

• Play loud music to psych them up

• Make the cells competent

• Incubate them for a long period of time

What is a plasmid?

• A circular piece of DNA

• A long strand of DNA, similar to a chromosome

• A strain of bacteria

• A very hot liquid

What was the role that calcium chloride played in the transformation?

• It helped the DNA enter the bacteria

• It helped the DNA bind/get close to the membranes of the bacteria

• It opened up the plasmid to allow us to insert genes

• It helped strengthen the bones of the E. Coli

What was the purpose of the "heat shock" treatment?

• It killed the E. Coli

• It made the E. Coli resistant to ampicillin

• It woke up the E. Coli from their hibernation

• It broke down the membranes of the E. Coli

The B-Lactamase gene provides bacteria with resistance to

(a)  

What is the abbreviation for the gene that allows the bacteria to glow?

• IPTG

• PGLO

• GFP

• GOP

What is the purpose of IPTG?

• It allows the GFP gene to be expressed

• It is an antibiotic that kills bacteria

• It is a plasmid that we are inserting into bacteria

• It is the yellow stuff that bacteria eat

What plates (should have) demonstrated bacterial growth?

• -DNA

• -DNA/amp

• +DNA/amp

• +DNA/amp/IPTG