CRISPR

What does CRISPR stand for?

• Clustered Regularly Interspaced Short Palindromic Repeats

• Customary Repeating Interactive Sequenced Popular Robots

• Creative Reading In Science Public Resources

• Clustered Regularly Indifferent Science Palindromic Repeats

What does CRISPR allow scientists to do?

• Delete Section of DNA Only

• Add New Sections of DNA Only

• Deleting, Adding, or Changing Sections of DNA

• Creating Life From Nothing

What is the role of Cas9?

• Glue sections of DNA together.

• Target mutated genes.

• Change the sequence or order of DNA bases.

• Cut DNA at specific locations.

Why is CRISPR the preferred gene editing tool?

• CRISPR is faster, cheaper and more accurate than the other forms of gene editing.

• CRISPR is faster but more expensive than other forms of gene editing.

• CRISPR is cheaper but not as accurate as other forms of gene editing.

• CRISPR is more accurate but slower than other forms of gene editing.

What is the difference between Somatic Modifications and Germline Modifications?

• Somatic deals with genes in non-reproductive cells and Germline deals with reproductive cells.

• Germline deals with genes in non-reproductive cells and Somatic deals with reproductive cells.

• Both deal with only reproductive cells.

• Both deal with only somatic cells which are not reproductive cells.

The use of CRISPR technology to boost our capabilities beyond the normal range of function is known as

• Enhancements

• Germline Transfer

• Somatic Cell Interference

• DNA Enzyme Manipulation

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria

Why do bacteria have CRISPR? Check all that apply.

• To be able to remember which viruses they have been infected with

• To be able to edit virus DNA

• To cut apart virus DNA thereby stopping the virus infection

• To be able to edit their own DNA

What is the role of the guide RNA?

• It helps the cell repair the DNA

• ensures that the Cas9 enzyme cuts at the right point in the genome

• Recognizes PAM sequences and opens up the DNA

• makes a cut across both strands of the DNA

How does the guide RNA help the cell know where to cut the DNA?

• Guide RNA helps to find the PAM

• Guide RNA is complementary to the DNA sequence

• Guide RNA always binds to the same sequence of DNA

• Like restriction sites, they are always palindromes