Protein purification

●Protein purification refers to the:

• Purification of different cellular components

• The process of converting proteins into their primary structure

• The process of separating and isolating a specific protein from a complex mixture

• The process of denaturing proteins.

●Protein separation techniques are often based on the following properties except

• Solubility of protein

• Viscosity of protein

• Charge  of the protein

• Specific binding affinity of the protein

●In which of the following separation method where proteins are separated based on their net charge

• Affinity chromatography

• Ion exchange chromatography

• Dialysis

• Gel filtration chromatography

●In gel filtration chromatography, the separation of proteins is based on their

  

• Size and net charge

• Size and shape

• Size and specific affinity

• Shape and net charge

●The use of insulin hormone to purify its receptor is an example of

• Ion exchange chromatography

• Affinity chromatography

• Gel filtration chromatography

• none of the above

In anion exchange chromatography

• The column contains negatively charged beads where positively charged proteins binds

• The column contains positively charged beads where negatively charged proteins bind

• The column contains both positive and negatively charged beads where proteins bind depending on their net charge

• All of these

The best method to hydrolyzed tryptophan  is

• Acid hydrolysis

• Base hydrolysis

• Salting out

• Enzymatic hydrolysis

●For the study of a protein in detail, an effort is usually made to first,

• Conjugate the protein to a known molecule

• Determination of amino acid composition

• Determination of amino acid sequence

• Purify the protein

What is the primary purpose of using an internal standard in an HPLC method?

• To increase the separation efficiency of the HPLC column

• To enhance the sensitivity of the HPLC detector.

• To monitor and correct for variations in sample preparation, injection volume, and detector response.

• To decrease the retention times of analytes on the HPLC column.

What is the primary benefit of using pre-column derivatization in HPLC for amino acid analysis?

• It reduces the need for sample preparation.

• It improves the separation of amino acids on the HPLC column.

• It allows for direct detection of native amino acids.

• It enhances the sensitivity and selectivity of detection.