
Protein purification
Authored by Roshali senarathne
Biology
University
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15 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
●Protein purification refers to the:
Purification of different cellular components
The process of converting proteins into their primary structure
The process of separating and isolating a specific protein from a complex mixture
The process of denaturing proteins.
2.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
●Protein separation techniques are often based on the following properties except
Solubility of protein
Viscosity of protein
Charge of the protein
Specific binding affinity of the protein
3.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
●In which of the following separation method where proteins are separated based on their net charge
Affinity chromatography
Ion exchange chromatography
Dialysis
Gel filtration chromatography
4.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
●In gel filtration chromatography, the separation of proteins is based on their
Size and net charge
Size and shape
Size and specific affinity
Shape and net charge
5.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
●The use of insulin hormone to purify its receptor is an example of
Ion exchange chromatography
Affinity chromatography
Gel filtration chromatography
none of the above
6.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
In anion exchange chromatography
The column contains negatively charged beads where positively charged proteins binds
The column contains positively charged beads where negatively charged proteins bind
The column contains both positive and negatively charged beads where proteins bind depending on their net charge
All of these
7.
MULTIPLE CHOICE QUESTION
30 sec • 2 pts
The best method to hydrolyzed tryptophan is
Acid hydrolysis
Base hydrolysis
Salting out
Enzymatic hydrolysis
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