MI 4.1.2c Control of Gene Expression

Inducible (a)   act like genetic on/off switches.

In the absence of an inducer, gene expression of an inducible promoter will be

• on

• off

• halfway on

In the PRESENCE of an inducer, gene expression of an inducible promoter will be

• on

• off

• halfway on

The inducer we will use in our lab to regulate GFP expression is known as

• IPTG

• GFP

• T7

• lac

The T7 expression host is

• a type of bacteria

• a plasmid

• a molecule

• a protein

The expression vector is

• a type of bacteria

• a plasmid

• a molecule

• a protein

This lab uses (a)   bacteria as the expression host. It is genetically engineered to express ​ (b)   under the control of the ​ (c)   promoter.

• E. coli
• T7 RNA polymerase
• lac
• S. pneumoniae
• DNA polymerase
• IPTG
• inducible

Tags

NGSS.HS-LS1-1

Under normal circumstances, T7 E. coli make (a)   molecules that bind to the ​ (b)   and block expression of T7 ​ (c)   . ​ ​

• repressor
• promoter
• polymerase
• transcription factor
• RNA

When present in the cell, IPTG binds to ​ (a)   which can then no longer bind to the ​ (b)   . Thus, ​ (c)   will be able to be expressed.​

• T7 expression host
• inducible promoter
• lac repressor
• lac promoter
• T7 polymerase
• plasmid
• GFP

A (a)   promoter is continuously transcribed and translated regardless of most cellular condition.