Protein Electrophoresis

What is the purpose of gel electrophoresis in protein analysis?

• To measure enzyme activity

• To amplify DNA sequences

• To determine cell viability

• To separate proteins based on size and charge for visualization and analysis.

Explain the procedure of gel electrophoresis in separating proteins.

• Proteins are separated based on shape and weight by applying an electric current to a gel matrix.

• Proteins are separated based on size and charge by applying an electric current to a gel matrix.

• Proteins are separated based on taste and smell by applying an electric current to a gel matrix.

• Proteins are separated based on color and texture by applying an electric current to a gel matrix.

What are the different protein separation techniques used in electrophoresis?

• Isoelectric focusing

• Gel filtration

• SDS-PAGE, Native PAGE, 2D-PAGE, Western blotting

• Reverse transcription

How do proteins get separated based on their size in gel electrophoresis?

• Based on the color of the proteins

• Based on the differential migration rates of proteins through the gel matrix under an electric field.

• Based on the alphabetical order of the proteins

• Based on the temperature of the gel

Describe the process of protein band analysis after electrophoresis.

• Protein band analysis includes measuring the pH of the gel

• Protein band analysis after electrophoresis involves staining the gel, capturing an image, and using software for quantification and comparison.

• Protein band analysis requires heating the gel before analysis

• Protein band analysis involves counting the number of bands observed

What is the role of electrophoresis equipment in protein separation?

• To separate proteins based on size and charge by applying an electric field.

• To analyze the genetic sequence of proteins

• To measure the concentration of proteins

• To mix proteins with other molecules

Why is it important to stain the protein bands after electrophoresis?

• To prevent contamination of the gel

• To visualize and analyze the separated proteins.

• To increase the speed of electrophoresis

• To add flavor to the proteins

What is the significance of the molecular weight marker in protein electrophoresis?

• Molecular weight markers are used to determine the pH of the gel

• Molecular weight markers serve as reference points for estimating the size of unknown proteins.

• Molecular weight markers help in separating proteins based on their charge

• Molecular weight markers assist in identifying the protein concentration

How does the pH of the gel affect protein separation in electrophoresis?

• The pH of the gel affects protein separation by altering the charge of the proteins, allowing them to migrate based on size and charge differences.

• Proteins separate in electrophoresis based on their taste, not pH

• The pH of the gel affects protein separation by changing the color of the proteins

• The pH of the gel has no impact on protein separation in electrophoresis

Explain the concept of isoelectric focusing in protein electrophoresis.

• Isoelectric focusing separates proteins based on their hydrophobicity by using a reverse-phase chromatography.

• Isoelectric focusing separates proteins based on their charge by using a size exclusion column.

• Isoelectric focusing separates proteins based on their molecular weight by using a pH gradient.

• Isoelectric focusing separates proteins based on their isoelectric point by using a pH gradient to immobilize proteins at their pI.