L-Asparaginase activity assay

At what temperature should ERM buffer be stored for long-term preservation?

• Room temperature

• 4° Celsius

• -20° Celsius

• -80° Celsius

At what temperature the ERM buffer works best at?

• 45° C

• 37° C

• 25° C

• 4° C

What is the absorbance wavelength required?

• 240nm

• 320nm

• 340nm

• 420nm

What are the components required for each run? [Multiple answers]

• Samples

• Calibrators

• Spike controls

• Positive controls

What are the calibrator concentrations used to create the standard curve?

• 500U/L, 450U/L, 400U/L, 350U/L, 300U/L, 250U/L

• 500U/L, 400U/L, 300U/L, 200U/L, 100U/L, 0U/L

• 500U/L, 250U/L, 175U/L, 87.5U/L, 43.75U/L, 0U/L

• 500U/L, 300U/L, 125U/L, 60U/L, 25U/L, 0U/L

What are the recommended starting sample dilutions to be run?

• 1x, 2x, 3x

• 1x, 3x, 5x

• 2x, 4x, 6x

• 3x, 5x, 8x

How much samples to be added into each well?

• 5ul

• 10ul

• 50ul

• 100ul

How much volume of ERM to be added per reaction well?

• 100 microlitre

• 190 microlitre

• 240 microlitre

• 290 microlitre