Transcription and RNA Synthesis Quiz

Synthesis of mRNA on DNA template is

• unidirectional

• bidirectional

• bidirectional with the help of primer

• unidirectional with the help of primer

Which of the following enzymes does NOT require a primer

• RNA dependent DNA polymerase

• DNA dependent DNA Polymerase

• DNA dependent RNA polymerase

• Taq DNA polymerase

Which one of the following statements about E. coli RNA polymerase is FALSE

• The holoenzyme includes the sigma factor

• The core enzyme includes the sigma factor

• It requires Mg2+ for its activity

• it requires Zn2+ for its activity

The DNA-binding protein that initiates the transcription of bacterial genes is called a/an

• operator

• promoter

• repressor

• sigma factor

In eukaryotes the ribosomal RNA genes are transcribed by

• reverse transcriptase

• RNA dependent RNA polymerase

• RNA polymerase I

• RNA polymerase III

A promoter of a typical eukaryotic gene is composed of

• a binding site for the sigma factor

• a binding site for TATA binding protein

• a binding site for transcription factor IID

• a binding site for transcription factor IIB

About 25-35 bp upstream of the transcriptional start site in most plant genes, is a short sequence, which serves as the site of assembly of the transcriptional initiation complex called

• CAAT box

• GC box

• TATA box

• repressor

Which one of the following transcription factors bind to TATA box?

• TFIID

• TFIIB

• TFIIA

• TFIIE

Which one of the following regions in the mature mRNA is not found in the gene for the mRNA?

• start codon

• leader sequence

• poly A tail

• exon

Mature eukaryotic mRNAs have a 5' cap that is a residue of

• 2-methylguanosine

• 3-methylguanosine

• 5-methylguanosine

• 7-methylguanosine