lab exam

Centrifuge

• Spins at a high speed to separate things by density (denser stuff moves lower in the tube).

• Bind metal ions so they can be removed from the sample.

• Can be programmed to keep samples/reactions at specific temperatures for specific lengths of time.

• Measures and transfers very specific, usually small, volumes of fluid accurately.

Thermocycler

• Spins at a high speed to separate things by density (denser stuff moves lower in the tube).

• Bind metal ions so they can be removed from the sample.

• Can be programmed to keep samples/reactions at specific temperatures for specific lengths of time.

• Measures and transfers very specific, usually small, volumes of fluid accurately.

Micropipette

• Spins at a high speed to separate things by density (denser stuff moves lower in the tube).

• Bind metal ions so they can be removed from the sample.

• Can be programmed to keep samples/reactions at specific temperatures for specific lengths of time.

• Measures and transfers very specific, usually small, volumes of fluid accurately.

Chelex beads

• Spins at a high speed to separate things by density (denser stuff moves lower in the tube).

• Bind metal ions so they can be removed from the sample.

• Can be programmed to keep samples/reactions at specific temperatures for specific lengths of time.

• Measures and transfers very specific, usually small, volumes of fluid accurately.

Why do the cheek cells need to be lysed before setting up PCR?

• Lysing marks the DNA so the enzymes in PCR can find it.

• Lysing destroys everything in the cells so they cannot divide.

• Lysing stains the cells so they can be measured.

• Lysing breaks open the cells so the DNA is accessible.

Extension

• The temperature is changed to the ideal temperature for Taq polymerase to build (synthesize) a new strand based on the template.

• The DNA template is heated so the strands separate.

• The machine that controls the temperature of the reaction and has been programmed to move through the heating and cooling steps.

• The reaction is cooled so the primers can stick to the DNA template.

Denaturation

• The temperature is changed to the ideal temperature for Taq polymerase to build (synthesize) a new strand based on the template.

• The DNA template is heated so the strands separate.

• The machine that controls the temperature of the reaction and has been programmed to move through the heating and cooling steps.

• The reaction is cooled so the primers can stick to the DNA template.

Thermocycler

• The temperature is changed to the ideal temperature for Taq polymerase to build (synthesize) a new strand based on the template.

• The DNA template is heated so the strands separate.

• The machine that controls the temperature of the reaction and has been programmed to move through the heating and cooling steps.

• The reaction is cooled so the primers can stick to the DNA template.

Annealing

• The temperature is changed to the ideal temperature for Taq polymerase to build (synthesize) a new strand based on the template.

• The DNA template is heated so the strands separate.

• The machine that controls the temperature of the reaction and has been programmed to move through the heating and cooling steps.

• The reaction is cooled so the primers can stick to the DNA template.

Which of the following will be components in your PCR mixture?  More than one answer may be correct.

• Master Mix

• Bradford Reagent

• Human DNA

• E. coli DNA