POLYMERASE CHAIN REACTION

The polymerase chain reaction is:

• a)It is a DNA sequencing technique.

• b)It is a DNA degradation technique.

• c)It is a DNA amplification process.

• d)All of the above.

Primer used for the process of polymerase chain reaction is

• a) Single stranded DNA oligonucleotide

• b) Double stranded DNA oligonucleotide

• c) Single stranded RNA oligonucleotide

• d) Double stranded RNA oligonucleotide

Polymerase used for PCR is extracted from

• a) Escherichia coli

• b) Home sapiens

• c) Thermus aquaticus

• d) Saccharomyces cerevisiae

What is the process of binding of primer to the denatured strand called?

• a)Annealing

• b)Denaturation

• c)Renaturation

• d)None of the above

At what temperature do denaturation of DNA double helix take place?

• a) 80^O C

• b) 72^O C

• c) 94^O C

• d) 54^O C

Who among the following created the PCR method?

• 1. a) Kary Mullis

• 1. b) Altman

• 1. c) Milstein

• 1. d) Kohler

Taq polymerase used in polymerase chain reaction is a

• a) RNA polymerase

• b) RNA ligase

• c) DNA polymerase

• d) DNA ligase