What is Polymerase Chain Reaction (PCR)? | miniPCR bio™

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What does PCR stand for?

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Polymerase Chain Reaction

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Which base does Adenine (A) pair with in DNA?

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Thymine (T)

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Why is Taq Polymerase used in PCR?

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Because it remains stable at high temperatures.

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What is the role of Taq Polymerase in PCR?

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It synthesizes new DNA strands during the extension phase.

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What is denaturation in the context of PCR?

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Denaturation involves heating the DNA to 90-98°C to separate the strands.

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What temperature is typically used for annealing in PCR?

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Typically between 50-65°C.

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What is the purpose of the annealing step in PCR?

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To allow primers to bind to the template DNA.

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What are primers in PCR?

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Short sequences of nucleotides that initiate DNA synthesis.

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What is the extension phase in PCR?

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The phase where Taq Polymerase adds nucleotides to the growing DNA strand.

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Where was Taq Polymerase originally found?

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In thermophilic bacteria in hot springs.