CRISPR-Cas9 Gene Editing Quiz

What does CRISPR stand for?

• Clustered Regularly Interspaced Short Palindromic Repeats

• Clustered Randomly Interspaced Short Palindromic Repeats

• Clustered Regularly Interspaced Short Protein Repeats

• Clustered Regularly Interspaced Short Palindrome Repeats

Tags

NGSS.HS-LS3-2

What is the primary function of the Cas9 enzyme in bacteria?

• To transcribe bacterial RNA

• To destroy invading viral DNA

• To replicate bacterial DNA

• To synthesize proteins

What is a PAM sequence?

• A sequence of nucleotides that codes for proteins

• A sequence that terminates RNA transcription

• A sequence of two-six nucleotides next to the DNA targeted by Cas9

• A sequence that initiates DNA replication

In CRISPR-Cas9 gene editing, what role does the Cas9 protein play?

• It unwinds the DNA helix

• It repairs the DNA after cutting

• It synthesizes sgRNA

• It binds to the sgRNA and cuts the target DNA

What is one major feature of CRISPR-Cas9 in gene editing?

• It only works in bacterial cells

• It requires a viral infection to function

• It randomly cuts DNA sequences

• It uses a synthetic gRNA to target specific DNA sequences

What is the role of Cas9 in genome editing?

• To act as molecular scissors cutting DNA at specific sites

• To replicate the genome

• To translate proteins from RNA

• To transcribe RNA from DNA

What happens to a gene that has been cut by a genome editing system?

• It is repaired by the cell's DNA repair machinery

• It is permanently deleted

• It is transcribed into RNA

• It is translated into a protein

What capability does CRISPR-Cas9 provide that was not possible in earlier gene therapy techniques?

• Random insertion of genes

• Transcription of RNA

• Precise editing of specific DNA sequences

• Replication of viral DNA

How could CRISPR-Cas9 be used to correct a mutation in the HBB gene causing sickle cell anemia?

• By transcribing the mutated gene into RNA

• By inserting a viral gene

• By deleting the entire HBB gene

• By cutting the mutated gene and allowing repair with a correct sequence

Tags

NGSS.HS-LS3-2

In CRISPR-Cas9 technology, the PAM sequence is:

• The site where Cas9 binds to

• 3 nucleotides in length

• All the above

• Identifies where Cas9 needs to unzip the double stranded DNA