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Which of the following would be PAS negative?

• basement membranes

• neutral polysaccharides

• acid mucins

• glycolipids

True of False? Alcian Blue pH1.0 demonstrates carboxylated mucosaccharides only.

• true

• false

Here a kidney that had PAS staining done on it, what could be possible reasons for this result?

What's a substitute stain to Alcian Blue?

• colloidal iron

• PAS

• PASD

• mucicarmine

While testing the Schiff's reagent, after adding a few drops of formalin, the solution turns magenta. This would indicate the reagent is ...​ (a)  

• good for use
• too old
• incorrect, the colour change should be blue

You perform the PAS stain correctly and all your reagents are good but the positive reaction isn't as strong as you want it to be. What can you do to intensify the reaction for next time?

Match the following results

• glycogen present

• PAS-, PASD +

• glycogen not present

• PAS +, PASD +

• invalid

• PAS -, PASD -

• troubleshoot

• PAS+, PASD -

After you performed the colloidal iron stain, you notice that you see blue specs all throughout the slide. What happened and what's a potential solution?

You perform a PAS and PASD stain on a bronchus, what is the expected result?

• PAS +, PASD -

• no positive reaction

• PAS -, PASD +

• PAS +, PASD +

Reorder the following chemicals for the PASD stain.

• Gill's Hx

• diastase digestion

• schiff's reagent

• periodic acid